6tj0 - Revision history

OCA at 11:31, 26 August 2020

OCA — Wed, 26 Aug 2020 11:31:46 GMT

←Older revision		Revision as of 11:31, 26 August 2020
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	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/BCSE_ECOLI BCSE_ECOLI]] Binds bis-(3'-5') cyclic diguanylic acid (c-di-GMP), the ability to bind c-di-GMP is important for its function (PubMed:24942809).<ref>PMID:24942809</ref>		[[http://www.uniprot.org/uniprot/BCSE_ECOLI BCSE_ECOLI]] Binds bis-(3'-5') cyclic diguanylic acid (c-di-GMP), the ability to bind c-di-GMP is important for its function (PubMed:24942809).<ref>PMID:24942809</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Most bacteria respond to surfaces by biogenesis of intracellular c-di-GMP, which inhibits motility and induces secretion of biofilm-promoting adherence factors. Bacterial cellulose is a widespread biofilm component whose secretion in Gram-negative species requires an inner membrane, c-di-GMP-dependent synthase tandem (BcsAB), an outer membrane porin (BcsC), and various accessory subunits that regulate synthase assembly and function as well as the exopolysaccharide's chemical composition and mechanical properties. We recently showed that in Escherichia coli, most Bcs proteins form a megadalton-sized secretory nanomachine, but the role and structure of individual regulatory components remained enigmatic. Here, we demonstrate that essential-for-secretion BcsR and BcsQ regulate each other's folding and stability and are recruited to the inner membrane via c-di-GMP-sensing BcsE and its intraoperon partner BcsF. Crystallographic and solution-based data show that BcsE's predicted GIL domain is a degenerate receiver-GGDEF domain tandem (BcsE(REC)(-GGDEF)), where the divergent diguanylate cyclase module binds both dimeric c-di-GMP and BcsQ through mutually independent interfaces. In addition, we reveal that a third N-terminal domain (BcsE(NTD)) determines the protein's homooligomerization and targeting of BcsERQ to the membrane as well as previously unreported interactions with transcription antitermination complex components. Together, the data suggest that BcsE acts on multiple levels to fine-tune bacterial cellulose secretion, from the early stages of secretion system assembly to the maintenance of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.IMPORTANCE Bacterial cellulose is a widespread biofilm component that can modulate microbial fitness and virulence both in the environment and infected hosts. Whereas its secretion generally involves an inner membrane c-di-GMP-dependent synthase tandem (BcsAB) across the bacterial domain of life, enterobacteria feature sophisticated Escherichia coli-like Bcs secretion systems, where multiple additional subunits are either required for secretion or contribute to the maximal production of the polysaccharide in vivo. Here, we demonstrate that essential-for-secretion BcsR and BcsQ regulate each other's folding and stability and are recruited to the inner membrane via c-di-GMP-sensing BcsE and its intraoperon partner, BcsF. Crystallographic and functional data reveal that BcsE features unexpected domain architecture and likely acts on multiple levels to fine-tune bacterial cellulose production, from the early stages of secretion system assembly to the maintenence of a membrane-proximal pool of dimeric c-di-GMP for processive synthase activation.
		+
		+	Structure and Multitasking of the c-di-GMP-Sensing Cellulose Secretion Regulator BcsE.,Zouhir S, Abidi W, Caleechurn M, Krasteva PV mBio. 2020 Aug 11;11(4). pii: mBio.01303-20. doi: 10.1128/mBio.01303-20. PMID:32788377<ref>PMID:32788377</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6tj0" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>

OCA at 05:53, 5 August 2020

OCA — Wed, 05 Aug 2020 05:53:27 GMT

←Older revision		Revision as of 05:53, 5 August 2020
Line 1:		Line 1:

-	==Crystal structure of the bacterial cellulose secretion regulator BcsE, residues 217-523, with bound c-di-GMP==	+	==Crystal structure of the bacterial cellulose secretion regulator BcsE, residues 217-523, with bound c-di-GMP.==
-	<StructureSection load='6tj0' size='340' side='right'caption='[[6tj0]]' scene=''>	+	<StructureSection load='6tj0' size='340' side='right'caption='[[6tj0]], [[Resolution\|resolution]] 2.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TJ0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TJ0 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6tj0]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Ecoli Ecoli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TJ0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TJ0 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tj0 OCA], [http://pdbe.org/6tj0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tj0 RCSB], [http://www.ebi.ac.uk/pdbsum/6tj0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tj0 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=C2E:9,9-[(2R,3R,3aS,5S,7aR,9R,10R,10aS,12S,14aR)-3,5,10,12-tetrahydroxy-5,12-dioxidooctahydro-2H,7H-difuro[3,2-d 3,2-j][1,3,7,9,2,8]tetraoxadiphosphacyclododecine-2,9-diyl]bis(2-amino-1,9-dihydro-6H-purin-6-one)'>C2E</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">bcsE, yhjS, b3536, JW3504 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=83333 ECOLI])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tj0 OCA], [http://pdbe.org/6tj0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tj0 RCSB], [http://www.ebi.ac.uk/pdbsum/6tj0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tj0 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/BCSE_ECOLI BCSE_ECOLI]] Binds bis-(3'-5') cyclic diguanylic acid (c-di-GMP), the ability to bind c-di-GMP is important for its function (PubMed:24942809).<ref>PMID:24942809</ref>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Ecoli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Abidi W]]	+	[[Category: Abidi, W]]
-	[[Category: Krasteva P]]	+	[[Category: Krasteva, P V]]
-	[[Category: Zouhir S]]	+	[[Category: Zouhir, S]]
		+	[[Category: Bacterial biofilm]]
		+	[[Category: Bacterial cellulose]]
		+	[[Category: Bacterial secretion system]]
		+	[[Category: C-di-gmp binding protein]]
		+	[[Category: Gil domain]]
		+	[[Category: Signaling protein]]

OCA at 11:08, 29 July 2020

OCA — Wed, 29 Jul 2020 11:08:43 GMT

←Older revision		Revision as of 11:08, 29 July 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6tj0 is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of the bacterial cellulose secretion regulator BcsE, residues 217-523, with bound c-di-GMP==
-		+	<StructureSection load='6tj0' size='340' side='right'caption='[[6tj0]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TJ0 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TJ0 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tj0 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tj0 OCA], [http://pdbe.org/6tj0 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tj0 RCSB], [http://www.ebi.ac.uk/pdbsum/6tj0 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tj0 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Abidi W]]
		+	[[Category: Krasteva P]]
		+	[[Category: Zouhir S]]

OCA at 13:33, 11 December 2019

OCA — Wed, 11 Dec 2019 13:33:11 GMT

←Older revision		Revision as of 13:33, 11 December 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6tj0 is ON HOLD	+	The entry 6tj0 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6tj0" [edit=sysop:move=sysop]

OCA — Wed, 04 Dec 2019 06:25:52 GMT

Protected "6tj0" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:25, 4 December 2019

OCA: New page: '''Unreleased structure''' The entry 6tj0 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 04 Dec 2019 06:25:50 GMT

New page: '''Unreleased structure''' The entry 6tj0 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6tj0 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]