OCA at 17:48, 10 March 2021

OCA — Wed, 10 Mar 2021 17:48:58 GMT

←Older revision		Revision as of 17:48, 10 March 2021
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	==Structure of HdrA-like subunit from Hyphomicrobium denitrificans==		==Structure of HdrA-like subunit from Hyphomicrobium denitrificans==
-	<StructureSection load='6tjr' size='340' side='right'caption='[[6tjr]]' scene=''>	+	<StructureSection load='6tjr' size='340' side='right'caption='[[6tjr]], [[Resolution\|resolution]] 1.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TJR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6TJR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6tjr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Atcc_51888 Atcc 51888]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TJR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TJR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6tjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tjr OCA], [~~http~~://pdbe.org/6tjr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6tjr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6tjr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6tjr ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FAD:FLAVIN-ADENINE+DINUCLEOTIDE'>FAD</scene>, <scene name='pdbligand=MES:2-(N-MORPHOLINO)-ETHANESULFONIC+ACID'>MES</scene>, <scene name='pdbligand=SF4:IRON/SULFUR+CLUSTER'>SF4</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">Hden_0691 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=53399 ATCC 51888])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tjr OCA], [https://pdbe.org/6tjr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tjr RCSB], [https://www.ebi.ac.uk/pdbsum/6tjr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tjr ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Many Bacteria and Archaea employ a novel pathway of sulfur oxidation involving an enzyme complex that is related to the heterodisulfide reductase (HdrABC) of methanogens. As a first step in the biochemical characterization of Hdr-like proteins from sulfur oxidizers (sHdr), we structurally analyzed the recombinant sHdrA protein from the Alphaproteobacterium Hyphomicrobium denitrificans at 1.4 A resolution. The sHdrA core structure is similar to that of methanogenic HdrA (mHdrA) which binds the electron-bifurcating flavin adenine dinucleotide (FAD), the heart of the HdrABC-[NiFe]-hydrogenase catalyzed reaction. Each sHdrA homodimer carries two FADs and two [4Fe-4S] clusters being linked by electron conductivity. Redox titrations monitored by electron paramagnetic resonance and visible spectroscopy revealed a redox potential between -203 and -188 mV for the [4Fe-4S] center. The potentials for the FADH/FADH(-) and FAD/FADH pairs reside between -174 and -156 mV and between -81 and -19 mV, respectively. The resulting stable semiquinone FADH* species, already detectable in the visible and EPR spectra of the as-isolated state of sHdrA is incompatible with basic principles of flavin-based electron bifurcation such that the sHdr complex does not apply this new mode of energy coupling. The inverted one-electron FAD redox potentials of sHdr and mHdr are clearly reflected in the different FAD-polypeptide interactions. According to this finding and the assumption that the sHdr complex forms an asymmetric HdrAA'B1C1B2C2 hexamer we tentatively propose a mechanism that links protein-bound sulfane oxidation to sulfite on HdrB1 with NAD(+) reduction via lipoamide disulfide reduction on HdrB2. The FAD of HdrA thereby serves as an electron storage unit.
		+
		+	Structural and spectroscopic characterization of a HdrA-like subunit from Hyphomicrobium denitrificans.,Ernst C, Kayastha K, Koch T, Venceslau SS, Pereira IAC, Demmer U, Ermler U, Dahl C FEBS J. 2020 Aug 4. doi: 10.1111/febs.15505. PMID:32750208<ref>PMID:32750208</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6tjr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 51888]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Dahl C]]	+	[[Category: Dahl, C]]
-	[[Category: Ermler U]]	+	[[Category: Ermler, U]]
-	[[Category: Kayastha K]]	+	[[Category: Kayastha, K]]
		+	[[Category: Dissimilatory sulfur oxidation]]
		+	[[Category: Electron bifurcation]]
		+	[[Category: Fad]]
		+	[[Category: Flavoprotein]]
		+	[[Category: Heterodisulfide reductase]]

OCA at 06:19, 19 August 2020

OCA — Wed, 19 Aug 2020 06:19:26 GMT

←Older revision		Revision as of 06:19, 19 August 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6tjr is ~~ON HOLD until Paper Publication~~	+	==Structure of HdrA-like subunit from Hyphomicrobium denitrificans==
-		+	<StructureSection load='6tjr' size='340' side='right'caption='[[6tjr]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TJR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TJR FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tjr OCA], [http://pdbe.org/6tjr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tjr RCSB], [http://www.ebi.ac.uk/pdbsum/6tjr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tjr ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Dahl C]]
		+	[[Category: Ermler U]]
		+	[[Category: Kayastha K]]

OCA at 06:16, 22 July 2020

OCA — Wed, 22 Jul 2020 06:16:40 GMT

←Older revision		Revision as of 06:16, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6tjr is ON HOLD	+	The entry 6tjr is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6tjr" [edit=sysop:move=sysop]

OCA — Wed, 04 Dec 2019 06:27:55 GMT

Protected "6tjr" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:27, 4 December 2019

OCA: New page: '''Unreleased structure''' The entry 6tjr is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 04 Dec 2019 06:27:53 GMT

New page: '''Unreleased structure''' The entry 6tjr is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6tjr is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6tjr - Revision history

OCA at 17:48, 10 March 2021

OCA at 06:19, 19 August 2020

OCA at 06:16, 22 July 2020

OCA: Protected "6tjr" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6tjr is ON HOLD Authors: Description: Category: Unreleased Structures