6tno - Revision history

OCA at 13:05, 24 January 2024

OCA — Wed, 24 Jan 2024 13:05:38 GMT

←Older revision		Revision as of 13:05, 24 January 2024
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-	====	+	==Crystal structure of the human Arc N-lobe bound to stargazin==
-	<StructureSection load='6tno' size='340' side='right'caption='[[6tno]]' scene=''>	+	<StructureSection load='6tno' size='340' side='right'caption='[[6tno]], [[Resolution\|resolution]] 1.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6tno]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Felis_catus Felis catus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TNO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TNO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tno FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tno OCA], [https://pdbe.org/6tno PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tno RCSB], [https://www.ebi.ac.uk/pdbsum/6tno PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tno ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.9Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tno FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tno OCA], [https://pdbe.org/6tno PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tno RCSB], [https://www.ebi.ac.uk/pdbsum/6tno PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tno ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/CCG2_HUMAN CCG2_HUMAN] Autosomal dominant non-syndromic intellectual disability. The disease is caused by variants affecting the gene represented in this entry.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/CCG2_HUMAN CCG2_HUMAN] Regulates the trafficking and gating properties of AMPA-selective glutamate receptors (AMPARs). Promotes their targeting to the cell membrane and synapses and modulates their gating properties by slowing their rates of activation, deactivation and desensitization. Does not show subunit-specific AMPA receptor regulation and regulates all AMPAR subunits. Thought to stabilize the calcium channel in an inactivated (closed) state.<ref>PMID:20805473</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The activity-regulated cytoskeleton-associated protein (Arc) is important for synaptic plasticity and the normal function of the brain. Arc interacts with neuronal postsynaptic proteins, but the mechanistic details of its function have not been fully established. The C-terminal domain of Arc consists of tandem domains, termed the N- and C-lobe. The N-lobe harbours a peptide binding site, able to bind multiple targets. By measuring the affinity of human Arc towards various peptides from stargazin and guanylate kinase-associated protein (GKAP), we have refined its specificity determinants. We found two sites in the GKAP repeat region that bind to Arc and confirmed these interactions by X-ray crystallography. Phosphorylation of the stargazin peptide did not affect binding affinity but caused changes in thermodynamic parameters. Comparison of the crystal structures of three high-resolution human Arc-peptide complexes identifies three conserved C-H...pi interactions at the binding cavity, explaining the sequence specificity of short linear motif binding by Arc. We further characterise central residues of the Arc lobe fold, show the effects of peptide binding on protein dynamics, and identify acyl carrier proteins as structures similar to the Arc lobes. We hypothesise that Arc may affect protein-protein interactions and phase separation at the postsynaptic density, affecting protein turnover and re-modelling of the synapse. The present data on Arc structure and ligand binding will help in further deciphering these processes.
		+
		+	Structural properties and peptide ligand binding of the capsid homology domains of human Arc.,Hallin EI, Bramham CR, Kursula P Biochem Biophys Rep. 2021 Mar 5;26:100975. doi: 10.1016/j.bbrep.2021.100975., eCollection 2021 Jul. PMID:33732907<ref>PMID:33732907</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6tno" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Felis catus]]
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Bramham CR]]
		+	[[Category: Hallin EI]]
		+	[[Category: Kursula P]]

OCA at 07:03, 7 April 2021

OCA — Wed, 07 Apr 2021 07:03:29 GMT

←Older revision		Revision as of 07:03, 7 April 2021
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	<StructureSection load='6tno' size='340' side='right'caption='[[6tno]]' scene=''>		<StructureSection load='6tno' size='340' side='right'caption='[[6tno]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6tno FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tno OCA], [~~http~~://pdbe.org/6tno PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6tno RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6tno PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6tno ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tno FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tno OCA], [https://pdbe.org/6tno PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tno RCSB], [https://www.ebi.ac.uk/pdbsum/6tno PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tno ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 12:31, 16 December 2020

OCA — Wed, 16 Dec 2020 12:31:07 GMT

←Older revision		Revision as of 12:31, 16 December 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6tno ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6tno' size='340' side='right'caption='[[6tno]]' scene=''>
-	~~Authors~~: ~~Hallin, E~~.I.~~, Bramham, C~~.R.~~, Kursula, P~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of the human Arc C-lobe~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tno FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tno OCA], [http://pdbe.org/6tno PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tno RCSB], [http://www.ebi.ac.uk/pdbsum/6tno PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tno ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Bramham~~, C.R]]	+	__TOC__
-	[[Category: ~~Hallin, E.I~~]]	+	</StructureSection>
-	[[Category: ~~Kursula, P~~]]	+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 06:19, 22 July 2020

OCA — Wed, 22 Jul 2020 06:19:17 GMT

←Older revision		Revision as of 06:19, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6tno is ON HOLD	+	The entry 6tno is ON HOLD until Paper Publication

	Authors: Hallin, E.I., Bramham, C.R., Kursula, P.		Authors: Hallin, E.I., Bramham, C.R., Kursula, P.

OCA: Protected "6tno" [edit=sysop:move=sysop]

OCA — Wed, 18 Dec 2019 05:52:32 GMT

Protected "6tno" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:52, 18 December 2019

OCA: New page: '''Unreleased structure''' The entry 6tno is ON HOLD Authors: Hallin, E.I., Bramham, C.R., Kursula, P. Description: Crystal structure of the human Arc C-lobe [[Category: Unreleased Str...

OCA — Wed, 18 Dec 2019 05:52:31 GMT

New page: '''Unreleased structure''' The entry 6tno is ON HOLD Authors: Hallin, E.I., Bramham, C.R., Kursula, P. Description: Crystal structure of the human Arc C-lobe [[Category: Unreleased Str...

New page

'''Unreleased structure'''

The entry 6tno is ON HOLD

Authors: Hallin, E.I., Bramham, C.R., Kursula, P.

Description: Crystal structure of the human Arc C-lobe
[[Category: Unreleased Structures]]
[[Category: Bramham, C.R]]
[[Category: Hallin, E.I]]
[[Category: Kursula, P]]