6tp8 - Revision history

OCA at 13:06, 24 January 2024

OCA — Wed, 24 Jan 2024 13:06:36 GMT

←Older revision		Revision as of 13:06, 24 January 2024
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	==Substrate protein interactions in the limbus region of the catalytic site of Candida antarctica Lipase B==		==Substrate protein interactions in the limbus region of the catalytic site of Candida antarctica Lipase B==
-	<StructureSection load='6tp8' size='340' side='right'caption='[[6tp8]]' scene=''>	+	<StructureSection load='6tp8' size='340' side='right'caption='[[6tp8]], [[Resolution\|resolution]] 1.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TP8 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6TP8 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6tp8]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Moesziomyces_antarcticus Moesziomyces antarcticus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TP8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TP8 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6tp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tp8 OCA], [~~http~~://pdbe.org/6tp8 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6tp8 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6tp8 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6tp8 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.55Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DEP:DIETHYL+PHOSPHONATE'>DEP</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=NTK:2,3-di(butanoyloxy)propyl+butanoate'>NTK</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tp8 OCA], [https://pdbe.org/6tp8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tp8 RCSB], [https://www.ebi.ac.uk/pdbsum/6tp8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tp8 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/LIPB_PSEA2 LIPB_PSEA2] Hydrolysis of triglycerides. Is very stereospecific both in hydrolysis and in organic synthesis and has a potentially important application in glucolipid synthesis.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Lipases (E.C. 3.1.1.3) are ubiquitous hydrolases for the carboxyl ester bond of water-insoluble substrates such as triacylglycerols and phospholipids. Candida antarctica Lipase B (CALB) acts in aqueous as well as in low-water media, thus being of considerable biochemical significance with high interest also for its industrial applications. The hydrolysis reaction follows a two-step mechanism, or 'interfacial activation', with adsorption of the enzyme to a heterogeneous interface and subsequent enhancement of the lipolytic activity. Once positioned within the catalytic triad, substrates are then hydrolysed, and products released. However, the intermediate steps of substrate transfer from the lipidic-aqueous phase to the enzyme surface and then down to the catalytic site are still unclear. By inhibiting CALB with ethyl phosphonate and incubating with glyceryl tributyrate (2,3-di(butanoyloxy)propyl butanoate), the crystal structure of the lipid-enzyme complex, at 1.55 A resolution, shows the tributyrin in the limbus region of active site. The substrate is found 10 A above the catalytic Ser, with the glycerol backbone pre-aligned for further processing by key interactions via an extended water network with alpha-helix10 and alpha-helix5. The findings offer new elements to elucidate the mechanism of substrate recognition, transfer and catalysis of Candida antarctica Lipase B (CALB) and lipases in general.
		+
		+	Principles of lipid-enzyme interactions in the limbus region of the catalytic site of Candida antarctica Lipase B.,Silvestrini L, Cianci M Int J Biol Macromol. 2020 May 4;158:358-363. doi: 10.1016/j.ijbiomac.2020.04.061. PMID:32380114<ref>PMID:32380114</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6tp8" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Lipase 3D Structures\|Lipase 3D Structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Moesziomyces antarcticus]]
	[[Category: Cianci M]]		[[Category: Cianci M]]
	[[Category: Silvestrini L]]		[[Category: Silvestrini L]]

OCA at 05:47, 20 May 2020

OCA — Wed, 20 May 2020 05:47:42 GMT

←Older revision		Revision as of 05:47, 20 May 2020
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-	'''Unreleased structure'''

-	~~The entry 6tp8 is ON HOLD until Dec 12 2021~~	+	==Substrate protein interactions in the limbus region of the catalytic site of Candida antarctica Lipase B==
-		+	<StructureSection load='6tp8' size='340' side='right'caption='[[6tp8]]' scene=''>
-	~~Authors: Cianci, M., Silvestrini, L.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TP8 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TP8 FirstGlance]. <br>
-	~~Description:~~ Substrate protein interactions in the limbus region of the catalytic site of Candida antarctica Lipase B	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tp8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tp8 OCA], [http://pdbe.org/6tp8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tp8 RCSB], [http://www.ebi.ac.uk/pdbsum/6tp8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tp8 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Cianci, M]]	+	__TOC__
-	[[Category: Silvestrini, L]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Cianci M]]
		+	[[Category: Silvestrini L]]

OCA at 13:17, 29 January 2020

OCA — Wed, 29 Jan 2020 13:17:02 GMT

←Older revision		Revision as of 13:17, 29 January 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6tp8 is ON HOLD	+	The entry 6tp8 is ON HOLD until Dec 12 2021

	Authors: Cianci, M., Silvestrini, L.		Authors: Cianci, M., Silvestrini, L.
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	Description: Substrate protein interactions in the limbus region of the catalytic site of Candida antarctica Lipase B		Description: Substrate protein interactions in the limbus region of the catalytic site of Candida antarctica Lipase B
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Silvestrini, L]]
	[[Category: Cianci, M]]		[[Category: Cianci, M]]
		+	[[Category: Silvestrini, L]]

OCA: Protected "6tp8" [edit=sysop:move=sysop]

OCA — Wed, 18 Dec 2019 05:57:49 GMT

Protected "6tp8" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:57, 18 December 2019

OCA: New page: '''Unreleased structure''' The entry 6tp8 is ON HOLD Authors: Cianci, M., Silvestrini, L. Description: Substrate protein interactions in the limbus region of the catalytic site of Cand...

OCA — Wed, 18 Dec 2019 05:57:47 GMT

New page: '''Unreleased structure''' The entry 6tp8 is ON HOLD Authors: Cianci, M., Silvestrini, L. Description: Substrate protein interactions in the limbus region of the catalytic site of Cand...

New page

'''Unreleased structure'''

The entry 6tp8 is ON HOLD

Authors: Cianci, M., Silvestrini, L.

Description: Substrate protein interactions in the limbus region of the catalytic site of Candida antarctica Lipase B
[[Category: Unreleased Structures]]
[[Category: Silvestrini, L]]
[[Category: Cianci, M]]