6tqq - Revision history

OCA at 07:38, 1 May 2024

OCA — Wed, 01 May 2024 07:38:54 GMT

←Older revision		Revision as of 07:38, 1 May 2024
Line 3:		Line 3:
	<StructureSection load='6tqq' size='340' side='right'caption='[[6tqq]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='6tqq' size='340' side='right'caption='[[6tqq]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6tqq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Tanapox_virus_strain_davis Tanapox~~ virus ~~strain davis~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TQQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TQQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6tqq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Yaba-like_disease_virus Yaba-like disease virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TQQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TQQ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.0017972Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style~~=~~'overflow: auto; max-height: 3em;'~~>~~[[6tqp\|6tqp]]~~<~~/div></td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">16L ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=132475 Tanapox virus strain Davis])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tqq OCA], [https://pdbe.org/6tqq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tqq RCSB], [https://www.ebi.ac.uk/pdbsum/6tqq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tqq ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tqq OCA], [https://pdbe.org/6tqq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tqq RCSB], [https://www.ebi.ac.uk/pdbsum/6tqq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tqq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/~~B2L11_HUMAN B2L11_HUMAN~~]] Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.<ref>PMID:9430630</ref> <ref>PMID:11734221</ref> <ref>PMID:12019181</ref> <ref>PMID:11997495</ref> <ref>PMID:15147734</ref> <ref>PMID:15486195</ref>	+	[https://www.uniprot.org/uniprot/Q9DHU6_YLDV Q9DHU6_YLDV]
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Premature programmed cell death or apoptosis of cells is a strategy utilized by multicellular organisms to counter microbial threats. Tanapoxvirus (TANV) is a large double-stranded DNA virus belonging to the poxviridae that causes mild monkeypox-like infections in humans and primates. TANV encodes for a putative apoptosis inhibitory protein 16L. We show that TANV16L is able to bind to a range of peptides spanning the BH3 motif of human proapoptotic Bcl-2 proteins and is able to counter growth arrest of yeast induced by human Bak and Bax. We then determined the crystal structures of TANV16L bound to three identified interactors, Bax, Bim and Puma BH3. TANV16L adopts a globular Bcl-2 fold comprising 7 alpha-helices and utilizes the canonical Bcl-2 binding groove to engage proapoptotic host cell Bcl-2 proteins. Unexpectedly, TANV16L is able to adopt both a monomeric and a domain-swapped dimeric topology where the alpha1 helix from one protomer is swapped into a neighbouring unit. Despite adopting two different oligomeric forms, the canonical ligand binding groove in TANV16L remains unchanged from monomer to domain-swapped dimer. Our results provide a structural and mechanistic basis for tanapoxvirus-mediated inhibition of host cell apoptosis and reveal the capacity of Bcl-2 proteins to adopt differential oligomeric states whilst maintaining the canonical ligand binding groove in an unchanged state. DATABASE: Structural data are available in the Protein Data Bank (PDB) under the accession numbers 6TPQ, 6TQQ and 6TRR.	+
-		+
-	Structural insight into tanapoxvirus-mediated inhibition of apoptosis.,Suraweera CD, Anasir MI, Chugh S, Javorsky A, Impey RE, Hasan Zadeh M, Soares da Costa TP, Hinds MG, Kvansakul M FEBS J. 2020 Sep;287(17):3733-3750. doi: 10.1111/febs.15365. Epub 2020 May 31. PMID:32412687<ref>PMID:32412687</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6tqq" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Tanapox~~ virus ~~strain davis~~]]	+	[[Category: Yaba-like disease virus]]
-	[[Category: Hinds~~, M G~~]]	+	[[Category: Hinds MG]]
-	[[Category: Kvansakul, M]]	+	[[Category: Kvansakul M]]
-	[[Category: Suraweera~~, C D]]~~	+	[[Category: Suraweera CD]]
-	~~[[Category: Apoptosis]]~~	+
-	~~[[Category: Bcl-2]]~~	+
-	~~[[Category: Pox virus~~]]	+

OCA at 15:19, 8 June 2021

OCA — Tue, 08 Jun 2021 15:19:00 GMT

←Older revision		Revision as of 15:19, 8 June 2021
Line 3:		Line 3:
	<StructureSection load='6tqq' size='340' side='right'caption='[[6tqq]], [[Resolution\|resolution]] 3.00Å' scene=''>		<StructureSection load='6tqq' size='340' side='right'caption='[[6tqq]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6tqq]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Tanapox_virus_strain_davis Tanapox virus strain davis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TQQ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6TQQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6tqq]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Tanapox_virus_strain_davis Tanapox virus strain davis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TQQ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6TQQ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6tqp\|6tqp]]</div></td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6tqp\|6tqp]]</div></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">16L ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=132475 Tanapox virus strain Davis])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">16L ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=132475 Tanapox virus strain Davis])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6tqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tqq OCA], [~~http~~://pdbe.org/6tqq PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6tqq RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6tqq PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6tqq ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6tqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tqq OCA], [https://pdbe.org/6tqq PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6tqq RCSB], [https://www.ebi.ac.uk/pdbsum/6tqq PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6tqq ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/B2L11_HUMAN B2L11_HUMAN]] Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.<ref>PMID:9430630</ref> <ref>PMID:11734221</ref> <ref>PMID:12019181</ref> <ref>PMID:11997495</ref> <ref>PMID:15147734</ref> <ref>PMID:15486195</ref>	+	[[https://www.uniprot.org/uniprot/B2L11_HUMAN B2L11_HUMAN]] Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.<ref>PMID:9430630</ref> <ref>PMID:11734221</ref> <ref>PMID:12019181</ref> <ref>PMID:11997495</ref> <ref>PMID:15147734</ref> <ref>PMID:15486195</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 12:47, 16 December 2020

OCA — Wed, 16 Dec 2020 12:47:20 GMT

←Older revision		Revision as of 12:47, 16 December 2020
Line 1:		Line 1:

	==Structural insight into tanapoxvirus mediated inhibition of apoptosis==		==Structural insight into tanapoxvirus mediated inhibition of apoptosis==
-	<StructureSection load='6tqq' size='340' side='right'caption='[[6tqq]]' scene=''>	+	<StructureSection load='6tqq' size='340' side='right'caption='[[6tqq]], [[Resolution\|resolution]] 3.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TQQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TQQ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6tqq]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Tanapox_virus_strain_davis Tanapox virus strain davis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TQQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TQQ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tqq OCA], [http://pdbe.org/6tqq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tqq RCSB], [http://www.ebi.ac.uk/pdbsum/6tqq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tqq ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6tqp\|6tqp]]</div></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">16L ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=132475 Tanapox virus strain Davis])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tqq OCA], [http://pdbe.org/6tqq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tqq RCSB], [http://www.ebi.ac.uk/pdbsum/6tqq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tqq ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/B2L11_HUMAN B2L11_HUMAN]] Induces apoptosis and anoikis. Isoform BimL is more potent than isoform BimEL. Isoform Bim-alpha1, isoform Bim-alpha2 and isoform Bim-alpha3 induce apoptosis, although less potent than isoform BimEL, isoform BimL and isoform BimS. Isoform Bim-gamma induces apoptosis. Isoform Bim-alpha3 induces apoptosis possibly through a caspase-mediated pathway. Isoform BimAC and isoform BimABC lack the ability to induce apoptosis.<ref>PMID:9430630</ref> <ref>PMID:11734221</ref> <ref>PMID:12019181</ref> <ref>PMID:11997495</ref> <ref>PMID:15147734</ref> <ref>PMID:15486195</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Premature programmed cell death or apoptosis of cells is a strategy utilized by multicellular organisms to counter microbial threats. Tanapoxvirus (TANV) is a large double-stranded DNA virus belonging to the poxviridae that causes mild monkeypox-like infections in humans and primates. TANV encodes for a putative apoptosis inhibitory protein 16L. We show that TANV16L is able to bind to a range of peptides spanning the BH3 motif of human proapoptotic Bcl-2 proteins and is able to counter growth arrest of yeast induced by human Bak and Bax. We then determined the crystal structures of TANV16L bound to three identified interactors, Bax, Bim and Puma BH3. TANV16L adopts a globular Bcl-2 fold comprising 7 alpha-helices and utilizes the canonical Bcl-2 binding groove to engage proapoptotic host cell Bcl-2 proteins. Unexpectedly, TANV16L is able to adopt both a monomeric and a domain-swapped dimeric topology where the alpha1 helix from one protomer is swapped into a neighbouring unit. Despite adopting two different oligomeric forms, the canonical ligand binding groove in TANV16L remains unchanged from monomer to domain-swapped dimer. Our results provide a structural and mechanistic basis for tanapoxvirus-mediated inhibition of host cell apoptosis and reveal the capacity of Bcl-2 proteins to adopt differential oligomeric states whilst maintaining the canonical ligand binding groove in an unchanged state. DATABASE: Structural data are available in the Protein Data Bank (PDB) under the accession numbers 6TPQ, 6TQQ and 6TRR.
		+
		+	Structural insight into tanapoxvirus-mediated inhibition of apoptosis.,Suraweera CD, Anasir MI, Chugh S, Javorsky A, Impey RE, Hasan Zadeh M, Soares da Costa TP, Hinds MG, Kvansakul M FEBS J. 2020 Sep;287(17):3733-3750. doi: 10.1111/febs.15365. Epub 2020 May 31. PMID:32412687<ref>PMID:32412687</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6tqq" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Suraweera, CD,~~ Hinds, ~~MG,~~ Kvansakul, M]]	+	[[Category: Tanapox virus strain davis]]
		+	[[Category: Hinds, M G]]
		+	[[Category: Kvansakul, M]]
		+	[[Category: Suraweera, C D]]
		+	[[Category: Apoptosis]]
		+	[[Category: Bcl-2]]
		+	[[Category: Pox virus]]

OCA at 06:28, 3 June 2020

OCA — Wed, 03 Jun 2020 06:28:11 GMT

←Older revision		Revision as of 06:28, 3 June 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6tqq is ON HOLD~~	+	==Structural insight into tanapoxvirus mediated inhibition of apoptosis==
-		+	<StructureSection load='6tqq' size='340' side='right'caption='[[6tqq]]' scene=''>
-	~~Authors: Suraweera, C.D., Hinds, M.G., Kvansakul, M.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6TQQ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6TQQ FirstGlance]. <br>
-	~~Description:~~ Structural insight into tanapoxvirus mediated inhibition of apoptosis	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6tqq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6tqq OCA], [http://pdbe.org/6tqq PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6tqq RCSB], [http://www.ebi.ac.uk/pdbsum/6tqq PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6tqq ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Kvansakul~~, M]]	+	__TOC__
-	[[Category: ~~Suraweera, C.D~~]]	+	</StructureSection>
-	[[Category: Hinds, M.G]]	+	[[Category: Large Structures]]
		+	[[Category: Suraweera, CD, Hinds, MG, Kvansakul, M]]

OCA: Protected "6tqq" [edit=sysop:move=sysop]

OCA — Wed, 25 Dec 2019 07:23:15 GMT

Protected "6tqq" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:23, 25 December 2019

OCA: New page: '''Unreleased structure''' The entry 6tqq is ON HOLD Authors: Suraweera, C.D., Hinds, M.G., Kvansakul, M. Description: Structural insight into tanapoxvirus mediated inhibition of apopt...

OCA — Wed, 25 Dec 2019 07:23:14 GMT

New page: '''Unreleased structure''' The entry 6tqq is ON HOLD Authors: Suraweera, C.D., Hinds, M.G., Kvansakul, M. Description: Structural insight into tanapoxvirus mediated inhibition of apopt...

New page

'''Unreleased structure'''

The entry 6tqq is ON HOLD

Authors: Suraweera, C.D., Hinds, M.G., Kvansakul, M.

Description: Structural insight into tanapoxvirus mediated inhibition of apoptosis
[[Category: Unreleased Structures]]
[[Category: Kvansakul, M]]
[[Category: Suraweera, C.D]]
[[Category: Hinds, M.G]]