6u07 - Revision history

OCA at 07:41, 11 October 2023

OCA — Wed, 11 Oct 2023 07:41:37 GMT

←Older revision		Revision as of 07:41, 11 October 2023
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	<StructureSection load='6u07' size='340' side='right'caption='[[6u07]], [[Resolution\|resolution]] 1.76Å' scene=''>		<StructureSection load='6u07' size='340' side='right'caption='[[6u07]], [[Resolution\|resolution]] 1.76Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6u07]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U07 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6U07 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u07]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U07 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U07 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.76Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~TRA@ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=9606 HUMAN]), B2M, HDCMA22P ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6u07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u07 OCA], [~~http~~://pdbe.org/6u07 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6u07 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6u07 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6u07 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u07 OCA], [https://pdbe.org/6u07 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u07 RCSB], [https://www.ebi.ac.uk/pdbsum/6u07 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u07 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q2YD82_HUMAN Q2YD82_HUMAN]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6u07" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6u07" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[T-cell receptor 3D structures\|T-cell receptor 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Austin, H]]	+	[[Category: Austin H]]
-	[[Category: Balasubramaniam, D]]	+	[[Category: Balasubramaniam D]]
-	[[Category: Chang, S]]	+	[[Category: Chang S]]
-	[[Category: Conner~~, E M~~]]	+	[[Category: Conner EM]]
-	[[Category: Demarest~~, S J~~]]	+	[[Category: Demarest SJ]]
-	[[Category: Dong, J]]	+	[[Category: Dong J]]
-	[[Category: Fitchett~~, J R~~]]	+	[[Category: Fitchett JR]]
-	[[Category: Frommelt~~, A J~~]]	+	[[Category: Frommelt AJ]]
-	[[Category: Froning, K]]	+	[[Category: Froning K]]
-	[[Category: Heng~~, A R~~]]	+	[[Category: Heng AR]]
-	[[Category: Hilgers~~, M T~~]]	+	[[Category: Hilgers MT]]
-	[[Category: Huang, F]]	+	[[Category: Huang F]]
-	[[Category: Kuhlman, B]]	+	[[Category: Kuhlman B]]
-	[[Category: Maguire, J]]	+	[[Category: Maguire J]]
-	[[Category: Sereno, A]]	+	[[Category: Sereno A]]
-	[[Category: Weichert, K]]	+	[[Category: Weichert K]]
-	[[Category: Wu, X]]	+	[[Category: Wu X]]
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: T cell receptor]]~~	+
-	~~[[Category: Tcr constant domain~~]]	+

OCA at 07:32, 27 May 2020

OCA — Wed, 27 May 2020 07:32:55 GMT

←Older revision		Revision as of 07:32, 27 May 2020
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	==Computational Stabilization of T Cell Receptor Constant Domains==		==Computational Stabilization of T Cell Receptor Constant Domains==
-	<StructureSection load='6u07' size='340' side='right'caption='[[6u07]]' scene=''>	+	<StructureSection load='6u07' size='340' side='right'caption='[[6u07]], [[Resolution\|resolution]] 1.76Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U07 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U07 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u07]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U07 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U07 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u07 OCA], [http://pdbe.org/6u07 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u07 RCSB], [http://www.ebi.ac.uk/pdbsum/6u07 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u07 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">TRA@ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), B2M, HDCMA22P ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u07 OCA], [http://pdbe.org/6u07 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u07 RCSB], [http://www.ebi.ac.uk/pdbsum/6u07 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u07 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Recombinant T cell receptors (TCRs) can be used to redirect naive T cells to eliminate virally infected or cancerous cells; however, they are plagued by low stability and uneven expression. Here, we use molecular modeling to identify mutations in the TCR constant domains (Calpha/Cbeta) that increase the unfolding temperature of Calpha/Cbeta by 20 degrees C, improve the expression of four separate alpha/beta TCRs by 3- to 10-fold, and improve the assembly and stability of TCRs with poor intrinsic stability. The stabilizing mutations rescue the expression of TCRs destabilized through variable domain mutation. The improved stability and folding of the TCRs reduces glycosylation, perhaps through conformational stabilization that restricts access to N-linked glycosylation enzymes. The Calpha/Cbeta mutations enables antibody-like expression and assembly of well-behaved bispecific molecules that combine an anti-CD3 antibody with the stabilized TCR. These TCR/CD3 bispecifics can redirect T cells to kill tumor cells with target HLA/peptide on their surfaces in vitro.
		+
		+	Computational stabilization of T cell receptors allows pairing with antibodies to form bispecifics.,Froning K, Maguire J, Sereno A, Huang F, Chang S, Weichert K, Frommelt AJ, Dong J, Wu X, Austin H, Conner EM, Fitchett JR, Heng AR, Balasubramaniam D, Hilgers MT, Kuhlman B, Demarest SJ Nat Commun. 2020 May 11;11(1):2330. doi: 10.1038/s41467-020-16231-7. PMID:32393818<ref>PMID:32393818</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6u07" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Austin H]]	+	[[Category: Austin, H]]
-	[[Category: Balasubramaniam D]]	+	[[Category: Balasubramaniam, D]]
-	[[Category: Chang S]]	+	[[Category: Chang, S]]
-	[[Category: Conner EM]]	+	[[Category: Conner, E M]]
-	[[Category: Demarest SJ]]	+	[[Category: Demarest, S J]]
-	[[Category: Dong J]]	+	[[Category: Dong, J]]
-	[[Category: Fitchett JR]]	+	[[Category: Fitchett, J R]]
-	[[Category: Frommelt AJ]]	+	[[Category: Frommelt, A J]]
-	[[Category: Froning K]]	+	[[Category: Froning, K]]
-	[[Category: Heng AR]]	+	[[Category: Heng, A R]]
-	[[Category: Hilgers MT]]	+	[[Category: Hilgers, M T]]
-	[[Category: Huang F]]	+	[[Category: Huang, F]]
-	[[Category: Kuhlman B]]	+	[[Category: Kuhlman, B]]
-	[[Category: Maguire J]]	+	[[Category: Maguire, J]]
-	[[Category: Sereno A]]	+	[[Category: Sereno, A]]
-	[[Category: Weichert K]]	+	[[Category: Weichert, K]]
-	[[Category: Wu X]]	+	[[Category: Wu, X]]
		+	[[Category: Immune system]]
		+	[[Category: T cell receptor]]
		+	[[Category: Tcr constant domain]]

OCA at 07:00, 15 April 2020

OCA — Wed, 15 Apr 2020 07:00:13 GMT

←Older revision		Revision as of 07:00, 15 April 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6u07 is ~~ON HOLD until Paper Publication~~	+	==Computational Stabilization of T Cell Receptor Constant Domains==
-		+	<StructureSection load='6u07' size='340' side='right'caption='[[6u07]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U07 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U07 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u07 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u07 OCA], [http://pdbe.org/6u07 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u07 RCSB], [http://www.ebi.ac.uk/pdbsum/6u07 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u07 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Austin H]]
		+	[[Category: Balasubramaniam D]]
		+	[[Category: Chang S]]
		+	[[Category: Conner EM]]
		+	[[Category: Demarest SJ]]
		+	[[Category: Dong J]]
		+	[[Category: Fitchett JR]]
		+	[[Category: Frommelt AJ]]
		+	[[Category: Froning K]]
		+	[[Category: Heng AR]]
		+	[[Category: Hilgers MT]]
		+	[[Category: Huang F]]
		+	[[Category: Kuhlman B]]
		+	[[Category: Maguire J]]
		+	[[Category: Sereno A]]
		+	[[Category: Weichert K]]
		+	[[Category: Wu X]]

OCA at 06:46, 1 January 2020

OCA — Wed, 01 Jan 2020 06:46:21 GMT

←Older revision		Revision as of 06:46, 1 January 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6u07 is ON HOLD	+	The entry 6u07 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 07:28, 25 December 2019

OCA — Wed, 25 Dec 2019 07:28:34 GMT

←Older revision		Revision as of 07:28, 25 December 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6u07 is ON HOLD ~~until Paper Publication~~	+	The entry 6u07 is ON HOLD

	Authors:		Authors:

OCA at 08:04, 2 October 2019

OCA — Wed, 02 Oct 2019 08:04:29 GMT

←Older revision		Revision as of 08:04, 2 October 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6u07 is ON HOLD	+	The entry 6u07 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6u07" [edit=sysop:move=sysop]

OCA — Wed, 21 Aug 2019 04:27:19 GMT

Protected "6u07" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:27, 21 August 2019

OCA: New page: '''Unreleased structure''' The entry 6u07 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 21 Aug 2019 04:27:17 GMT

New page: '''Unreleased structure''' The entry 6u07 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6u07 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]