6u18 - Revision history

OCA at 07:42, 11 October 2023

OCA — Wed, 11 Oct 2023 07:42:19 GMT

←Older revision		Revision as of 07:42, 11 October 2023
Line 3:		Line 3:
	<StructureSection load='6u18' size='340' side='right'caption='[[6u18]], [[Resolution\|resolution]] 2.00Å' scene=''>		<StructureSection load='6u18' size='340' side='right'caption='[[6u18]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6u18]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U18 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U18 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u18]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U18 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U18 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CTY:CLARITHROMYCIN'>CTY</scene>~~, ~~<scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">mphR(A), mphR, A9R57_28010, ACN77_02555, ACN77_03810, ACN77_19745, AKG29_00740, AM465_27045, APT94_14580, B1K96_22845, B9M99_28120, B9N33_26285, B9T59_28650, BANRA_01808, BANRA_05330, BANRA_05395, BE963_00530, BEN53_26070, BET08_23460, BFD68_26430, BJJ90_27670, BK248_28255, BK292_28800, BK334_26705, BK373_25645, BK375_28450, BK383_16415, BK400_25090, BVL39_07785, BVL39_26565, C5N07_27930, C5P01_27745, C5P43_14800, C5P44_28315, C6669_28285, C6986_31345, C7235_25300, C7235_26770, CQP61_00550, CR538_26710, CRT43_27530, CRT46_27035, CWS33_26295, D0X26_00055, D0X26_28400, D3O91_28140, D9N32_01235, DB359_27590, DBY14_07355, DBY14_07475, DBY14_07595, DBY14_07715, DBY14_07835, DBY14_07955, DBY14_08075, DBY14_08195, DBY14_08400, DBY14_09000, DBY14_09120, DBY14_09240, DBY14_09360, DBY14_09480, DBY14_09600, DBY14_09720, DBY14_09840, DBY14_09960, DBY14_10080, DBY14_10200, DBY14_10320, DBY14_10440, DBY14_10560, DBY14_10680, DBY14_10800, DBY14_10920, DBY14_11040, DBY14_11160, DBY14_11280, DL545_01125, DL545_26270, DM102_26995, DM129_26600, DM129_28140, DNQ41_27340, DS732_30515, E4Z89_24290, Eco118UI_26585, ECONIH1_26780, ECTO124_05297, EGT48_01470, EIA21_26670, EJC48_00900, EJC75_00560, EJH97_25820, EPS71_24560, EQ823_24865, EQ830_27025, ERS085366_04056, ERS085404_05054, ERS150873_04751, ETN48_p0081, EVY21_24885, ExPECSC022_02556, ExPECSC036_03428, ExPECSC065_05093, EXX13_27025, EYD11_23970, FORC82_p029, HmCmsJML146_04799, HMPREF3040_01640, MS6198_B124, MS8345_A00020, NCTC13846_04922, U12A_A00163, U14A_A00115, UN86_19865, YDC107_4984 ([https:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=562 "Bacillus coli" Migula 1895])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CTY:CLARITHROMYCIN'>CTY</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u18 OCA], [https://pdbe.org/6u18 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u18 RCSB], [https://www.ebi.ac.uk/pdbsum/6u18 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u18 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u18 OCA], [https://pdbe.org/6u18 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u18 RCSB], [https://www.ebi.ac.uk/pdbsum/6u18 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u18 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9EVJ6_ECOLX Q9EVJ6_ECOLX]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895~~]]	+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cropp~~, T A~~]]	+	[[Category: Cropp TA]]
-	[[Category: Li, Y]]	+	[[Category: Li Y]]
-	[[Category: Reed, M]]	+	[[Category: Reed M]]
-	[[Category: Williams, G]]	+	[[Category: Williams G]]
-	[[Category: Wright~~, H T]]~~	+	[[Category: Wright HT]]
-	~~[[Category: Biosensor]]~~	+
-	~~[[Category: Dna binding protein-antibiotic complex]]~~	+
-	~~[[Category: Dna-binding]]~~	+
-	~~[[Category: Macrolide~~]]	+

OCA at 12:44, 13 October 2021

OCA — Wed, 13 Oct 2021 12:44:11 GMT

←Older revision		Revision as of 12:44, 13 October 2021
Line 1:		Line 1:

	==Directed evolution of a biosensor selective for the macrolide antibiotic clarithromycin==		==Directed evolution of a biosensor selective for the macrolide antibiotic clarithromycin==
-	<StructureSection load='6u18' size='340' side='right'caption='[[6u18]]' scene=''>	+	<StructureSection load='6u18' size='340' side='right'caption='[[6u18]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U18 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6U18 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u18]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U18 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U18 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6u18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u18 OCA], [~~http~~://pdbe.org/6u18 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6u18 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6u18 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6u18 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CTY:CLARITHROMYCIN'>CTY</scene>, <scene name='pdbligand=FLC:CITRATE+ANION'>FLC</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mphR(A), mphR, A9R57_28010, ACN77_02555, ACN77_03810, ACN77_19745, AKG29_00740, AM465_27045, APT94_14580, B1K96_22845, B9M99_28120, B9N33_26285, B9T59_28650, BANRA_01808, BANRA_05330, BANRA_05395, BE963_00530, BEN53_26070, BET08_23460, BFD68_26430, BJJ90_27670, BK248_28255, BK292_28800, BK334_26705, BK373_25645, BK375_28450, BK383_16415, BK400_25090, BVL39_07785, BVL39_26565, C5N07_27930, C5P01_27745, C5P43_14800, C5P44_28315, C6669_28285, C6986_31345, C7235_25300, C7235_26770, CQP61_00550, CR538_26710, CRT43_27530, CRT46_27035, CWS33_26295, D0X26_00055, D0X26_28400, D3O91_28140, D9N32_01235, DB359_27590, DBY14_07355, DBY14_07475, DBY14_07595, DBY14_07715, DBY14_07835, DBY14_07955, DBY14_08075, DBY14_08195, DBY14_08400, DBY14_09000, DBY14_09120, DBY14_09240, DBY14_09360, DBY14_09480, DBY14_09600, DBY14_09720, DBY14_09840, DBY14_09960, DBY14_10080, DBY14_10200, DBY14_10320, DBY14_10440, DBY14_10560, DBY14_10680, DBY14_10800, DBY14_10920, DBY14_11040, DBY14_11160, DBY14_11280, DL545_01125, DL545_26270, DM102_26995, DM129_26600, DM129_28140, DNQ41_27340, DS732_30515, E4Z89_24290, Eco118UI_26585, ECONIH1_26780, ECTO124_05297, EGT48_01470, EIA21_26670, EJC48_00900, EJC75_00560, EJH97_25820, EPS71_24560, EQ823_24865, EQ830_27025, ERS085366_04056, ERS085404_05054, ERS150873_04751, ETN48_p0081, EVY21_24885, ExPECSC022_02556, ExPECSC036_03428, ExPECSC065_05093, EXX13_27025, EYD11_23970, FORC82_p029, HmCmsJML146_04799, HMPREF3040_01640, MS6198_B124, MS8345_A00020, NCTC13846_04922, U12A_A00163, U14A_A00115, UN86_19865, YDC107_4984 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 "Bacillus coli" Migula 1895])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u18 OCA], [https://pdbe.org/6u18 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u18 RCSB], [https://www.ebi.ac.uk/pdbsum/6u18 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u18 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Clarithromycin is an improved semisynthetic analogue of the naturally occurring macrolide, erythromycin. The subtle modification of a methyl group on the C-6 hydroxyl group endows the molecule with improved acid stability and results in a clinically useful antibiotic. Here, we show that the effector specificity of the biosensor protein, MphR, can be evolved to selectively recognize clarithromycin and therefore report on the production of this molecule in vivo. In addition, a crystal structure of the evolved variant reveals the molecular basis for selectivity and provides a guide for the evolution of a new metabolic function using this biosensor.
		+
		+	Development of Genetically Encoded Biosensors for Reporting the Methyltransferase-Dependent Biosynthesis of Semisynthetic Macrolide Antibiotics.,Li Y, Reed M, Wright HT, Cropp TA, Williams GJ ACS Synth Biol. 2021 Sep 21. doi: 10.1021/acssynbio.1c00151. PMID:34546703<ref>PMID:34546703</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6u18" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cropp TA]]	+	[[Category: Cropp, T A]]
-	[[Category: Li Y]]	+	[[Category: Li, Y]]
-	[[Category: Reed M]]	+	[[Category: Reed, M]]
-	[[Category: Williams G]]	+	[[Category: Williams, G]]
-	[[Category: Wright HT]]	+	[[Category: Wright, H T]]
		+	[[Category: Biosensor]]
		+	[[Category: Dna binding protein-antibiotic complex]]
		+	[[Category: Dna-binding]]
		+	[[Category: Macrolide]]

OCA at 06:20, 19 August 2020

OCA — Wed, 19 Aug 2020 06:20:10 GMT

←Older revision		Revision as of 06:20, 19 August 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6u18 ~~is ON HOLD until Paper Publication~~	+	==Directed evolution of a biosensor selective for the macrolide antibiotic clarithromycin==
-		+	<StructureSection load='6u18' size='340' side='right'caption='[[6u18]]' scene=''>
-	~~Authors~~: Li, Y., ~~Reed, M~~., ~~Wright, H~~.T., ~~Cropp, T~~.A., ~~Williams, G~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U18 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U18 FirstGlance]. <br>
-	~~Description: Directed evolution of a biosensor selective for the macrolide antibiotic clarithromycin~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u18 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u18 OCA], [http://pdbe.org/6u18 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u18 RCSB], [http://www.ebi.ac.uk/pdbsum/6u18 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u18 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Cropp~~, T.A~~]]	+	__TOC__
-	[[Category: ~~Williams, G~~]]	+	</StructureSection>
-	[[Category: ~~Li, Y~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Wright, H.T~~]]	+	[[Category: Cropp TA]]
-	[[Category: ~~Reed, M~~]]	+	[[Category: Li Y]]
		+	[[Category: Reed M]]
		+	[[Category: Williams G]]
		+	[[Category: Wright HT]]

OCA at 08:05, 2 October 2019

OCA — Wed, 02 Oct 2019 08:05:51 GMT

←Older revision		Revision as of 08:05, 2 October 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6u18 is ON HOLD	+	The entry 6u18 is ON HOLD until Paper Publication

	Authors: Li, Y., Reed, M., Wright, H.T., Cropp, T.A., Williams, G.		Authors: Li, Y., Reed, M., Wright, H.T., Cropp, T.A., Williams, G.
Line 9:		Line 9:
	[[Category: Cropp, T.A]]		[[Category: Cropp, T.A]]
	[[Category: Williams, G]]		[[Category: Williams, G]]
-	[[Category: Reed, M]]
	[[Category: Li, Y]]		[[Category: Li, Y]]
	[[Category: Wright, H.T]]		[[Category: Wright, H.T]]
		+	[[Category: Reed, M]]

OCA: Protected "6u18" [edit=sysop:move=sysop]

OCA — Wed, 21 Aug 2019 04:31:03 GMT

Protected "6u18" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:31, 21 August 2019

OCA: New page: '''Unreleased structure''' The entry 6u18 is ON HOLD Authors: Li, Y., Reed, M., Wright, H.T., Cropp, T.A., Williams, G. Description: Directed evolution of a biosensor selective for the...

OCA — Wed, 21 Aug 2019 04:31:02 GMT

New page: '''Unreleased structure''' The entry 6u18 is ON HOLD Authors: Li, Y., Reed, M., Wright, H.T., Cropp, T.A., Williams, G. Description: Directed evolution of a biosensor selective for the...

New page

'''Unreleased structure'''

The entry 6u18 is ON HOLD

Authors: Li, Y., Reed, M., Wright, H.T., Cropp, T.A., Williams, G.

Description: Directed evolution of a biosensor selective for the macrolide antibiotic clarithromycin
[[Category: Unreleased Structures]]
[[Category: Cropp, T.A]]
[[Category: Williams, G]]
[[Category: Reed, M]]
[[Category: Li, Y]]
[[Category: Wright, H.T]]