6u1q - Revision history

OCA at 07:42, 11 October 2023

OCA — Wed, 11 Oct 2023 07:42:36 GMT

←Older revision		Revision as of 07:42, 11 October 2023
Line 3:		Line 3:
	<StructureSection load='6u1q' size='340' side='right'caption='[[6u1q]], [[Resolution\|resolution]] 2.87Å' scene=''>		<StructureSection load='6u1q' size='340' side='right'caption='[[6u1q]], [[Resolution\|resolution]] 2.87Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6u1q]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U1Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6U1Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u1q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Vibrio_cholerae Vibrio cholerae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U1Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U1Q FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=PTR:O~~-~~PHOSPHOTYROSINE'>PTR</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.87Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>vpsO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~666~~ "~~Bacillo virgola del Koch~~" ~~Trevisan 1884])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6u1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u1q OCA], [~~http~~://pdbe.org/6u1q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6u1q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6u1q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6u1q ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u1q OCA], [https://pdbe.org/6u1q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u1q RCSB], [https://www.ebi.ac.uk/pdbsum/6u1q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u1q ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9KTG5_VIBCH Q9KTG5_VIBCH]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 21:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Bacillo virgola del koch trevisan 1884]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Brown~~, E R~~]]	+	[[Category: Vibrio cholerae]]
-	[[Category: Herbert, K]]	+	[[Category: Brown ER]]
-	[[Category: Porcella~~, M E~~]]	+	[[Category: Herbert K]]
-	[[Category: Rubin~~, S M~~]]	+	[[Category: Porcella ME]]
-	[[Category: Schwechheimer, C]]	+	[[Category: Rubin SM]]
-	[[Category: Tripathi~~, S M~~]]	+	[[Category: Schwechheimer C]]
-	[[Category: Yildiz~~, F H]]~~	+	[[Category: Tripathi SM]]
-	~~[[Category: Auto-phosphorylation]]~~	+	[[Category: Yildiz FH]]
-	~~[[Category: Biosynthetic protein]]~~	+
-	~~[[Category: Kinase]]~~	+
-	~~[[Category: Polysaccharide synthesis]]~~	+
-	~~[[Category: Tyrosine kinase~~]]	+

OCA at 06:37, 7 October 2020

OCA — Wed, 07 Oct 2020 06:37:45 GMT

←Older revision		Revision as of 06:37, 7 October 2020
Line 1:		Line 1:

	==Crystal Structure of VpsO (VC0937) Kinase domain==		==Crystal Structure of VpsO (VC0937) Kinase domain==
-	<StructureSection load='6u1q' size='340' side='right'caption='[[6u1q]]' scene=''>	+	<StructureSection load='6u1q' size='340' side='right'caption='[[6u1q]], [[Resolution\|resolution]] 2.87Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U1Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U1Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u1q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillo_virgola_del_koch"_trevisan_1884 "bacillo virgola del koch" trevisan 1884]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U1Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U1Q FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u1q OCA], [http://pdbe.org/6u1q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u1q RCSB], [http://www.ebi.ac.uk/pdbsum/6u1q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u1q ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">vpsO ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=666 "Bacillo virgola del Koch" Trevisan 1884])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u1q OCA], [http://pdbe.org/6u1q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u1q RCSB], [http://www.ebi.ac.uk/pdbsum/6u1q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u1q ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Production of an extracellular matrix is essential for biofilm formation, as this matrix both secures and protects the cells it encases. Mechanisms underlying production and assembly of matrices are poorly understood. Vibrio cholerae, relies heavily on biofilm formation for survival, infectivity, and transmission. Biofilm formation requires Vibrio polysaccharide (VPS), which is produced by vps gene-products, yet the function of these products remains unknown. Here, we demonstrate that the vps gene-products vpsO and vpsU encode respectively for a tyrosine kinase and a cognate tyrosine phosphatase. Collectively, VpsO and VpsU act as a tyrosine phosphoregulatory system to modulate VPS production. We present structures of VpsU and the kinase domain of VpsO, and we report observed autocatalytic tyrosine phosphorylation of the VpsO C-terminal tail. The position and amount of tyrosine phosphorylation in the VpsO C-terminal tail represses VPS production and biofilm formation through a mechanism involving the modulation of VpsO oligomerization. We found that tyrosine phosphorylation enhances stability of VpsO. Regulation of VpsO phosphorylation by the phosphatase VpsU is vital for maintaining native VPS levels. This study provides new insights into the mechanism and regulation of VPS production and establishes general principles of biofilm matrix production and its inhibition.
		+
		+	A tyrosine phosphoregulatory system controls exopolysaccharide biosynthesis and biofilm formation in Vibrio cholerae.,Schwechheimer C, Hebert K, Tripathi S, Singh PK, Floyd KA, Brown ER, Porcella ME, Osorio J, Kiblen JTM, Pagliai FA, Drescher K, Rubin SM, Yildiz FH PLoS Pathog. 2020 Aug 25;16(8):e1008745. doi: 10.1371/journal.ppat.1008745., eCollection 2020 Aug. PMID:32841296<ref>PMID:32841296</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6u1q" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillo virgola del koch trevisan 1884]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Brown ER]]	+	[[Category: Brown, E R]]
-	[[Category: Herbert K]]	+	[[Category: Herbert, K]]
-	[[Category: Porcella ME]]	+	[[Category: Porcella, M E]]
-	[[Category: Rubin SM]]	+	[[Category: Rubin, S M]]
-	[[Category: Schwechheimer C]]	+	[[Category: Schwechheimer, C]]
-	[[Category: Tripathi SM]]	+	[[Category: Tripathi, S M]]
-	[[Category: Yildiz FH]]	+	[[Category: Yildiz, F H]]
		+	[[Category: Auto-phosphorylation]]
		+	[[Category: Biosynthetic protein]]
		+	[[Category: Kinase]]
		+	[[Category: Polysaccharide synthesis]]
		+	[[Category: Tyrosine kinase]]

OCA at 06:20, 19 August 2020

OCA — Wed, 19 Aug 2020 06:20:16 GMT

←Older revision		Revision as of 06:20, 19 August 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6u1q ~~is ON HOLD until Paper Publication~~	+	==Crystal Structure of VpsO (VC0937) Kinase domain==
-		+	<StructureSection load='6u1q' size='340' side='right'caption='[[6u1q]]' scene=''>
-	~~Authors~~: ~~Tripathi, S~~.M.~~, Schwechheimer, C~~.~~, Herbert, K~~., ~~Porcella, M~~.E., ~~Brown~~, E.R., ~~Yildiz, F~~.H., ~~Rubin, S~~.M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U1Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U1Q FirstGlance]. <br>
-	~~Description: Crystal Structure of VpsO (VC0937) Kinase domain~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u1q OCA], [http://pdbe.org/6u1q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u1q RCSB], [http://www.ebi.ac.uk/pdbsum/6u1q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u1q ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Porcella, M.E~~]]	+	__TOC__
-	[[Category: ~~Yildiz, F.H~~]]	+	</StructureSection>
-	[[Category: ~~Rubin, S.M~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Tripathi, S.M~~]]	+	[[Category: Brown ER]]
-	[[Category: Schwechheimer, C]]	+	[[Category: Herbert K]]
-	[[Category: ~~Brown, E.R~~]]	+	[[Category: Porcella ME]]
-	[[Category: ~~Herbert, K~~]]	+	[[Category: Rubin SM]]
		+	[[Category: Schwechheimer C]]
		+	[[Category: Tripathi SM]]
		+	[[Category: Yildiz FH]]

OCA: Protected "6u1q" [edit=sysop:move=sysop]

OCA — Wed, 28 Aug 2019 14:01:15 GMT

Protected "6u1q" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:01, 28 August 2019

OCA: New page: '''Unreleased structure''' The entry 6u1q is ON HOLD until Paper Publication Authors: Tripathi, S.M., Schwechheimer, C., Herbert, K., Porcella, M.E., Brown, E.R., Yildiz, F.H., Rubin, S...

OCA — Wed, 28 Aug 2019 14:01:13 GMT

New page: '''Unreleased structure''' The entry 6u1q is ON HOLD until Paper Publication Authors: Tripathi, S.M., Schwechheimer, C., Herbert, K., Porcella, M.E., Brown, E.R., Yildiz, F.H., Rubin, S...

New page

'''Unreleased structure'''

The entry 6u1q is ON HOLD until Paper Publication

Authors: Tripathi, S.M., Schwechheimer, C., Herbert, K., Porcella, M.E., Brown, E.R., Yildiz, F.H., Rubin, S.M.

Description: Crystal Structure of VpsO (VC0937) Kinase domain
[[Category: Unreleased Structures]]
[[Category: Porcella, M.E]]
[[Category: Yildiz, F.H]]
[[Category: Rubin, S.M]]
[[Category: Tripathi, S.M]]
[[Category: Schwechheimer, C]]
[[Category: Brown, E.R]]
[[Category: Herbert, K]]