6u23 - Revision history

OCA at 06:38, 11 April 2020

OCA — Sat, 11 Apr 2020 06:38:05 GMT

←Older revision		Revision as of 06:38, 11 April 2020
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	<SX load='6u23' size='340' side='right' viewer='molstar' caption='[[6u23]], [[Resolution\|resolution]] 3.49Å' scene=''>		<SX load='6u23' size='340' side='right' viewer='molstar' caption='[[6u23]], [[Resolution\|resolution]] 3.49Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6u23]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U23 OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6U23 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u23]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U23 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U23 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MPEG1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MPEG1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6u23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u23 OCA], [http://pdbe.org/6u23 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u23 RCSB], [http://www.ebi.ac.uk/pdbsum/6u23 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u23 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u23 OCA], [http://pdbe.org/6u23 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u23 RCSB], [http://www.ebi.ac.uk/pdbsum/6u23 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u23 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 01:05, 7 March 2020

OCA — Sat, 07 Mar 2020 01:05:13 GMT

←Older revision		Revision as of 01:05, 7 March 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6u23 is ~~ON HOLD~~	+	==EM structure of MPEG-1(w.t.) soluble pre-pore==
		+	<SX load='6u23' size='340' side='right' viewer='molstar' caption='[[6u23]], [[Resolution\|resolution]] 3.49Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6u23]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U23 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6U23 FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MPEG1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6u23 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u23 OCA], [http://pdbe.org/6u23 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u23 RCSB], [http://www.ebi.ac.uk/pdbsum/6u23 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u23 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MPEG1_HUMAN MPEG1_HUMAN]] Plays a key role in the innate immune response following bacterial infection by inserting into the bacterial surface to form pores (By similarity). By breaching the surface of phagocytosed bacteria, allows antimicrobial effectors to enter the bacterial periplasmic space and degrade bacterial proteins such as superoxide dismutase sodC which contributes to bacterial virulence (By similarity). Shows antibacterial activity against a wide spectrum of Gram-positive, Gram-negative and acid-fast bacteria (PubMed:23753625, PubMed:26402460, PubMed:30609079). Reduces the viability of the intracytosolic pathogen L.monocytogenes by inhibiting acidification of the phagocytic vacuole of host cells which restricts bacterial translocation from the vacuole to the cytosol (By similarity). Required for the antibacterial activity of reactive oxygen species and nitric oxide (By similarity).[UniProtKB:A1L314]<ref>PMID:23753625</ref> <ref>PMID:26402460</ref> <ref>PMID:30609079</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Macrophage-expressed gene 1 (MPEG1/Perforin-2) is a perforin-like protein that functions within the phagolysosome to damage engulfed microbes. MPEG1 is thought to form pores in target membranes, however, its mode of action remains unknown. We use cryo-Electron Microscopy (cryo-EM) to determine the 2.4 A structure of a hexadecameric assembly of MPEG1 that displays the expected features of a soluble prepore complex. We further discover that MPEG1 prepore-like assemblies can be induced to perforate membranes through acidification, such as would occur within maturing phagolysosomes. We next solve the 3.6 A cryo-EM structure of MPEG1 in complex with liposomes. These data reveal that a multi-vesicular body of 12 kDa (MVB12)-associated beta-prism (MABP) domain binds membranes such that the pore-forming machinery of MPEG1 is oriented away from the bound membrane. This unexpected mechanism of membrane interaction suggests that MPEG1 remains bound to the phagolysosome membrane while simultaneously forming pores in engulfed bacterial targets.

-	~~Authors~~:	+	The cryo-EM structure of the acid activatable pore-forming immune effector Macrophage-expressed gene 1.,Pang SS, Bayly-Jones C, Radjainia M, Spicer BA, Law RHP, Hodel AW, Parsons ES, Ekkel SM, Conroy PJ, Ramm G, Venugopal H, Bird PI, Hoogenboom BW, Voskoboinik I, Gambin Y, Sierecki E, Dunstone MA, Whisstock JC Nat Commun. 2019 Sep 19;10(1):4288. doi: 10.1038/s41467-019-12279-2. PMID:31537793<ref>PMID:31537793</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6u23" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</SX>
		+	[[Category: Human]]
		+	[[Category: Large Structures]]
		+	[[Category: Bayly-Jones, C]]
		+	[[Category: Pang, S S]]
		+	[[Category: Immune system]]
		+	[[Category: Immunity]]
		+	[[Category: Macpf]]
		+	[[Category: Macrophage]]
		+	[[Category: Mpeg-1/perforin-2]]
		+	[[Category: Pore forming protein]]

OCA: Protected "6u23" [edit=sysop:move=sysop]

OCA — Wed, 28 Aug 2019 14:02:30 GMT

Protected "6u23" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:02, 28 August 2019

OCA: New page: '''Unreleased structure''' The entry 6u23 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 28 Aug 2019 14:02:28 GMT

New page: '''Unreleased structure''' The entry 6u23 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6u23 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]