6u5v - Revision history

OCA at 06:40, 11 April 2020

OCA — Sat, 11 Apr 2020 06:40:37 GMT

←Older revision		Revision as of 06:40, 11 April 2020
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	<SX load='6u5v' size='340' side='right' viewer='molstar' caption='[[6u5v]], [[Resolution\|resolution]] 2.80Å' scene=''>		<SX load='6u5v' size='340' side='right' viewer='molstar' caption='[[6u5v]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6u5v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U5V OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6U5V FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u5v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U5V OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U5V FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6u5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u5v OCA], [http://pdbe.org/6u5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u5v RCSB], [http://www.ebi.ac.uk/pdbsum/6u5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u5v ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u5v OCA], [http://pdbe.org/6u5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u5v RCSB], [http://www.ebi.ac.uk/pdbsum/6u5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u5v ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 01:07, 7 March 2020

OCA — Sat, 07 Mar 2020 01:07:25 GMT

←Older revision		Revision as of 01:07, 7 March 2020
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	==Electron cryomicroscopy Structure of C. albicans FAS in the Apo state==		==Electron cryomicroscopy Structure of C. albicans FAS in the Apo state==
-	<~~StructureSection~~ load='6u5v' size='340' side='right'caption='[[6u5v]], [[Resolution\|resolution]] 2.80Å' scene=''>	+	<SX load='6u5v' size='340' side='right' viewer='molstar' caption='[[6u5v]], [[Resolution\|resolution]] 2.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6u5v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6U5V FirstGlance]. <br>		<table><tr><td colspan='2'>[[6u5v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6U5V FirstGlance]. <br>
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	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Candida albicans]]		[[Category: Candida albicans]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 06:05, 16 October 2019

OCA — Wed, 16 Oct 2019 06:05:51 GMT

←Older revision		Revision as of 06:05, 16 October 2019
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-	'''Unreleased structure'''

-	~~The entry~~ 6u5v is ~~ON HOLD~~	+	==Electron cryomicroscopy Structure of C. albicans FAS in the Apo state==
		+	<StructureSection load='6u5v' size='340' side='right'caption='[[6u5v]], [[Resolution\|resolution]] 2.80Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6u5v]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Candida_albicans Candida albicans]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U5V OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6U5V FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=FMN:FLAVIN+MONONUCLEOTIDE'>FMN</scene>, <scene name='pdbligand=PNS:4-PHOSPHOPANTETHEINE'>PNS</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6u5v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u5v OCA], [http://pdbe.org/6u5v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u5v RCSB], [http://www.ebi.ac.uk/pdbsum/6u5v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u5v ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/FAS2_CANAX FAS2_CANAX]] Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The alpha subunit contains domains for: acyl carrier protein, 3-oxoacyl-[acyl-carrier-protein] reductase, and 3-oxoacyl-[acyl-carrier-protein] synthase. [[http://www.uniprot.org/uniprot/FAS1_CANAX FAS1_CANAX]] Fatty acid synthetase catalyzes the formation of long-chain fatty acids from acetyl-CoA, malonyl-CoA and NADPH. The beta subunit contains domains for: [acyl-carrier-protein] acetyltransferase and malonyltransferase, S-acyl fatty acid synthase thioesterase, enoyl-[acyl-carrier-protein] reductase, and 3-hydroxypalmitoyl-[acyl-carrier-protein] dehydratase.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	During fatty acid biosynthesis, acyl carrier proteins (ACPs) from type I fungal fatty acid synthase (FAS) shuttle substrates and intermediates within a reaction chamber that hosts multiple spatially-fixed catalytic centers. A major challenge in understanding the mechanism of ACP-mediated substrate shuttling is experimental observation of its transient interaction landscape within the reaction chamber. Here, we have shown that ACP spatial distribution is sensitive to the presence of substrates in a catalytically inhibited state, which enables high-resolution investigation of the ACP-dependent conformational transitions within the enoyl reductase (ER) reaction site. In two fungal FASs with distinct ACP localization, the shuttling domain is targeted to the ketoacyl-synthase (KS) domain and away from other catalytic centers, such as acetyl-transferase (AT) and ER domains by steric blockage of the KS active site followed by addition of substrates. These studies strongly suggest that acylation of phosphopantetheine arm of ACP may be an integral part of the substrate shuttling mechanism in type I fungal FAS.

-	~~Authors:~~ Lou, ~~J.W.~~, Mazhab-Jafari~~, M~~.T.	+	Electron cryomicroscopy observation of acyl carrier protein translocation in type I fungal fatty acid synthase.,Lou JW, Iyer KR, Hasan SMN, Cowen LE, Mazhab-Jafari MT Sci Rep. 2019 Sep 10;9(1):12987. doi: 10.1038/s41598-019-49261-3. PMID:31506493<ref>PMID:31506493</ref>

-	~~Description: Electron cryomicroscopy Structure~~ of C. albicans ~~FAS in the Apo state~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
-	[[Category: Lou, J.W]]	+	<div class="pdbe-citations 6u5v" style="background-color:#fffaf0;"></div>
-	[[Category: Mazhab-Jafari, M.T]]	+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Candida albicans]]
		+	[[Category: Large Structures]]
		+	[[Category: Lou, J W]]
		+	[[Category: Mazhab-Jafari, M T]]
		+	[[Category: Fungal fatty acid synthase]]
		+	[[Category: Transferase]]

OCA: Protected "6u5v" [edit=sysop:move=sysop]

OCA — Wed, 02 Oct 2019 08:15:47 GMT

Protected "6u5v" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:15, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6u5v is ON HOLD Authors: Lou, J.W., Mazhab-Jafari, M.T. Description: Electron cryomicroscopy Structure of C. albicans FAS in the Apo state [[Catego...

OCA — Wed, 02 Oct 2019 08:15:46 GMT

New page: '''Unreleased structure''' The entry 6u5v is ON HOLD Authors: Lou, J.W., Mazhab-Jafari, M.T. Description: Electron cryomicroscopy Structure of C. albicans FAS in the Apo state [[Catego...

New page

'''Unreleased structure'''

The entry 6u5v is ON HOLD

Authors: Lou, J.W., Mazhab-Jafari, M.T.

Description: Electron cryomicroscopy Structure of C. albicans FAS in the Apo state
[[Category: Unreleased Structures]]
[[Category: Lou, J.W]]
[[Category: Mazhab-Jafari, M.T]]