6u6g - Revision history

OCA at 10:48, 27 March 2024

OCA — Wed, 27 Mar 2024 10:48:43 GMT

←Older revision		Revision as of 10:48, 27 March 2024
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6u6g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_truncatula Medicago truncatula]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U6G FirstGlance]. <br>		<table><tr><td colspan='2'>[[6u6g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_truncatula Medicago truncatula]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U6G FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u6g OCA], [https://pdbe.org/6u6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u6g RCSB], [https://www.ebi.ac.uk/pdbsum/6u6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u6g ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u6g OCA], [https://pdbe.org/6u6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u6g RCSB], [https://www.ebi.ac.uk/pdbsum/6u6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u6g ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[https://www.uniprot.org/uniprot/A0A396GSL0_MEDTR A0A396GSL0_MEDTR]		[https://www.uniprot.org/uniprot/A0A396GSL0_MEDTR A0A396GSL0_MEDTR]
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	In the indeterminate nodules of a model legume Medicago truncatula, approximately 700 nodule-specific cysteine-rich (NCR) peptides with conserved cysteine signature are expressed. NCR peptides are highly diverse in sequence, and some of these cationic peptides exhibit antimicrobial activity in vitro and in vivo. However, there is a lack of knowledge regarding their structural architecture, antifungal activity, and modes of action against plant fungal pathogens. Here, the three-dimensional NMR structure of the 36-amino acid NCR044 peptide was solved. This unique structure was largely disordered and highly dynamic with one four-residue alpha-helix and one three-residue antiparallel beta-sheet stabilized by two disulfide bonds. NCR044 peptide also exhibited potent fungicidal activity against multiple plant fungal pathogens, including Botrytis cinerea and three Fusarium spp. It inhibited germination in quiescent spores of B. cinerea In germlings, it breached the fungal plasma membrane and induced reactive oxygen species. It bound to multiple bioactive phosphoinositides in vitro. Time-lapse confocal and superresolution microscopy revealed strong fungal cell wall binding, penetration of the cell membrane at discrete foci, followed by gradual loss of turgor, subsequent accumulation in the cytoplasm, and elevated levels in nucleoli of germlings. Spray-applied NCR044 significantly reduced gray mold disease symptoms caused by the fungal pathogen B. cinerea in tomato and tobacco plants, and postharvest products. Our work illustrates the antifungal activity of a structurally unique NCR peptide against plant fungal pathogens and paves the way for future development of this class of peptides as a spray-on fungistat/fungicide.
-
-	Antifungal symbiotic peptide NCR044 exhibits unique structure and multifaceted mechanisms of action that confer plant protection.,Velivelli SLS, Czymmek KJ, Li H, Shaw JB, Buchko GW, Shah DM Proc Natl Acad Sci U S A. 2020 Jul 7;117(27):16043-16054. doi:, 10.1073/pnas.2003526117. Epub 2020 Jun 22. PMID:32571919<ref>PMID:32571919</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 6u6g" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:04, 14 June 2023

OCA — Wed, 14 Jun 2023 11:04:42 GMT

←Older revision		Revision as of 11:04, 14 June 2023
Line 1:		Line 1:

	==Solution NMR structure of the nodule-specific cysteine-rich peptide NCR044 from Medicago truncatula==		==Solution NMR structure of the nodule-specific cysteine-rich peptide NCR044 from Medicago truncatula==
-	<StructureSection load='6u6g' size='340' side='right'caption='[[6u6g~~]], [[NMR_Ensembles_of_Models \| 19 NMR models~~]]' scene=''>	+	<StructureSection load='6u6g' size='340' side='right'caption='[[6u6g]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6u6g]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Barrel_medic Barrel medic~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U6G OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6U6G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u6g]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Medicago_truncatula Medicago truncatula]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U6G OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U6G FirstGlance]. <br>
-	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MtrunA17_Chr7g0216231 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3880 Barrel medic])</td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u6g OCA], [https://pdbe.org/6u6g PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u6g RCSB], [https://www.ebi.ac.uk/pdbsum/6u6g PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u6g ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6u6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u6g OCA], [~~http~~://pdbe.org/6u6g PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6u6g RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6u6g PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6u6g ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A396GSL0_MEDTR A0A396GSL0_MEDTR]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 21:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Barrel medic]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Buchko, G W~~]]	+	[[Category: Medicago truncatula]]
-	[[Category: ~~Shah, D M~~]]	+	[[Category: Buchko GW]]
-	[[Category: ~~Antifungal agent]]~~	+	[[Category: Shah DM]]
-	~~[[Category: Antifungal protein]]~~	+
-	~~[[Category: Defensin-like]]~~	+
-	~~[[Category: Fungal disease~~]]	+

OCA at 08:35, 21 October 2020

OCA — Wed, 21 Oct 2020 08:35:31 GMT

←Older revision		Revision as of 08:35, 21 October 2020
Line 3:		Line 3:
	<StructureSection load='6u6g' size='340' side='right'caption='[[6u6g]], [[NMR_Ensembles_of_Models \| 19 NMR models]]' scene=''>		<StructureSection load='6u6g' size='340' side='right'caption='[[6u6g]], [[NMR_Ensembles_of_Models \| 19 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6u6g]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U6G OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6U6G FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u6g]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Barrel_medic Barrel medic]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U6G OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U6G FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6u6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u6g OCA], [http://pdbe.org/6u6g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u6g RCSB], [http://www.ebi.ac.uk/pdbsum/6u6g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u6g ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">MtrunA17_Chr7g0216231 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=3880 Barrel medic])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u6g OCA], [http://pdbe.org/6u6g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u6g RCSB], [http://www.ebi.ac.uk/pdbsum/6u6g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u6g ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	In the indeterminate nodules of a model legume Medicago truncatula, approximately 700 nodule-specific cysteine-rich (NCR) peptides with conserved cysteine signature are expressed. NCR peptides are highly diverse in sequence, and some of these cationic peptides exhibit antimicrobial activity in vitro and in vivo. However, there is a lack of knowledge regarding their structural architecture, antifungal activity, and modes of action against plant fungal pathogens. Here, the three-dimensional NMR structure of the 36-amino acid NCR044 peptide was solved. This unique structure was largely disordered and highly dynamic with one four-residue alpha-helix and one three-residue antiparallel beta-sheet stabilized by two disulfide bonds. NCR044 peptide also exhibited potent fungicidal activity against multiple plant fungal pathogens, including Botrytis cinerea and three Fusarium spp. It inhibited germination in quiescent spores of B. cinerea In germlings, it breached the fungal plasma membrane and induced reactive oxygen species. It bound to multiple bioactive phosphoinositides in vitro. Time-lapse confocal and superresolution microscopy revealed strong fungal cell wall binding, penetration of the cell membrane at discrete foci, followed by gradual loss of turgor, subsequent accumulation in the cytoplasm, and elevated levels in nucleoli of germlings. Spray-applied NCR044 significantly reduced gray mold disease symptoms caused by the fungal pathogen B. cinerea in tomato and tobacco plants, and postharvest products. Our work illustrates the antifungal activity of a structurally unique NCR peptide against plant fungal pathogens and paves the way for future development of this class of peptides as a spray-on fungistat/fungicide.
		+
		+	Antifungal symbiotic peptide NCR044 exhibits unique structure and multifaceted mechanisms of action that confer plant protection.,Velivelli SLS, Czymmek KJ, Li H, Shaw JB, Buchko GW, Shah DM Proc Natl Acad Sci U S A. 2020 Jul 7;117(27):16043-16054. doi:, 10.1073/pnas.2003526117. Epub 2020 Jun 22. PMID:32571919<ref>PMID:32571919</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6u6g" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Barrel medic]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Buchko, G W]]		[[Category: Buchko, G W]]

OCA at 05:29, 10 October 2019

OCA — Thu, 10 Oct 2019 05:29:22 GMT

←Older revision		Revision as of 05:29, 10 October 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6u6g is ON HOLD~~	+	==Solution NMR structure of the nodule-specific cysteine-rich peptide NCR044 from Medicago truncatula==
-		+	<StructureSection load='6u6g' size='340' side='right'caption='[[6u6g]], [[NMR_Ensembles_of_Models \| 19 NMR models]]' scene=''>
-	~~Authors: Buchko, G.W., Shah, D.M.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6u6g]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U6G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6U6G FirstGlance]. <br>
-	~~Description:~~ Solution NMR structure of the nodule-specific cysteine-rich peptide NCR044 from Medicago truncatula	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6u6g FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u6g OCA], [http://pdbe.org/6u6g PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u6g RCSB], [http://www.ebi.ac.uk/pdbsum/6u6g PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u6g ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Shah, D.M]]	+	__TOC__
-	[[Category: ~~Buchko, G.W~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Buchko, G W]]
		+	[[Category: Shah, D M]]
		+	[[Category: Antifungal agent]]
		+	[[Category: Antifungal protein]]
		+	[[Category: Defensin-like]]
		+	[[Category: Fungal disease]]

OCA: Protected "6u6g" [edit=sysop:move=sysop]

OCA — Wed, 02 Oct 2019 08:17:33 GMT

Protected "6u6g" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:17, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6u6g is ON HOLD Authors: Buchko, G.W., Shah, D.M. Description: Solution NMR structure of the nodule-specific cysteine-rich peptide NCR044 from Medi...

OCA — Wed, 02 Oct 2019 08:17:31 GMT

New page: '''Unreleased structure''' The entry 6u6g is ON HOLD Authors: Buchko, G.W., Shah, D.M. Description: Solution NMR structure of the nodule-specific cysteine-rich peptide NCR044 from Medi...

New page

'''Unreleased structure'''

The entry 6u6g is ON HOLD

Authors: Buchko, G.W., Shah, D.M.

Description: Solution NMR structure of the nodule-specific cysteine-rich peptide NCR044 from Medicago truncatula
[[Category: Unreleased Structures]]
[[Category: Shah, D.M]]
[[Category: Buchko, G.W]]