6u6q - Revision history

OCA at 07:39, 1 May 2024

OCA — Wed, 01 May 2024 07:39:06 GMT

←Older revision		Revision as of 07:39, 1 May 2024
Line 1:		Line 1:

	==Solution NMR Structure Of The Partially Activated MTS Deleted Form MinE Protein (delta10-ngMinE) From Neisseria gonorrheae==		==Solution NMR Structure Of The Partially Activated MTS Deleted Form MinE Protein (delta10-ngMinE) From Neisseria gonorrheae==
-	<StructureSection load='6u6q' size='340' side='right'caption='[[6u6q~~]], [[NMR_Ensembles_of_Models \| 10 NMR models~~]]' scene=''>	+	<StructureSection load='6u6q' size='340' side='right'caption='[[6u6q]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6u6q]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"diplococcus_gonorrhoeae"_(zopf_1885)_lehmann_and_neumann_1896 "diplococcus~~ gonorrhoeae~~" (zopf 1885) lehmann and neumann 1896~~]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U6Q OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6U6Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u6q]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Neisseria_gonorrhoeae Neisseria gonorrhoeae]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U6Q OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U6Q FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>minE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~485~~ "~~Diplococcus gonorrhoeae~~" ~~(Zopf 1885) Lehmann and Neumann 1896])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Solution NMR</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6u6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u6q OCA], [~~http~~://pdbe.org/6u6q PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6u6q RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6u6q PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6u6q ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u6q OCA], [https://pdbe.org/6u6q PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u6q RCSB], [https://www.ebi.ac.uk/pdbsum/6u6q PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u6q ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MINE_NEIGO MINE_NEIGO]] Prevents the cell division inhibition by proteins MinC and MinD at internal division sites while permitting inhibition at polar sites. This ensures cell division at the proper site by restricting the formation of a division septum at the midpoint of the long axis of the cell (By similarity).	+	[https://www.uniprot.org/uniprot/MINE_NEIGO MINE_NEIGO] Prevents the cell division inhibition by proteins MinC and MinD at internal division sites while permitting inhibition at polar sites. This ensures cell division at the proper site by restricting the formation of a division septum at the midpoint of the long axis of the cell (By similarity).
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Bacterial MinD and MinE form a standing oscillatory wave which positions the cell division inhibitor MinC, that binds MinD, everywhere on the membrane except at the midpoint of the cell, ensuring midcell positioning of the cytokinetic septum. During this process MinE undergoes fold switching as it interacts with different partners. We explore the exchange dynamics between major and excited states of the MinE dimer in 3 forms using (15)N relaxation dispersion NMR: the full-length protein (6-stranded beta-sheet sandwiched between 4 helices) representing the resting state; a 10-residue N-terminal deletion (Delta10) mimicking the membrane-binding competent state where the N-terminal helix is detached to interact with membrane; and N-terminal deletions of either 30 (Delta30) or 10 residues with an I24N mutation (Delta10/I24N), in which the beta1-strands at the dimer interface are extruded and available to bind MinD, leaving behind a 4-stranded beta-sheet. Full-length MinE samples 2 "excited" states: The first is similar to a full-length/Delta10 heterodimer; the second, also sampled by Delta10, is either similar to or well along the pathway toward the 4-stranded beta-sheet form. Both Delta30 and Delta10/I24N sample 2 excited species: The first may involve destabilization of the beta3- and beta3'-strands at the dimer interface; changes in the second are more extensive, involving further disruption of secondary structure, possibly representing an ensemble of states on the pathway toward restoration of the resting state. The quantitative information on MinE conformational dynamics involving these excited states is crucial for understanding the oscillation pattern self-organization by MinD-MinE interaction dynamics on the membrane.	+
-		+
-	Probing transient excited states of the bacterial cell division regulator MinE by relaxation dispersion NMR spectroscopy.,Cai M, Huang Y, Shen Y, Li M, Mizuuchi M, Ghirlando R, Mizuuchi K, Clore GM Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25446-25455. doi:, 10.1073/pnas.1915948116. Epub 2019 Nov 26. PMID:31772021<ref>PMID:31772021</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6u6q" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Cai, M~~]]	+	[[Category: Neisseria gonorrhoeae]]
-	[[Category: ~~Clore,~~ M]]	+	[[Category: Cai M]]
-	[[Category: ~~Shen, Y~~]]	+	[[Category: Clore M]]
-	[[Category: ~~Cell cycle]]~~	+	[[Category: Shen Y]]
-	~~[[Category: Cell division]]~~	+
-	~~[[Category: Mind]]~~	+
-	~~[[Category: Mine~~]]	+

OCA at 08:21, 20 January 2021

OCA — Wed, 20 Jan 2021 08:21:30 GMT

←Older revision		Revision as of 08:21, 20 January 2021
Line 1:		Line 1:

	==Solution NMR Structure Of The Partially Activated MTS Deleted Form MinE Protein (delta10-ngMinE) From Neisseria gonorrheae==		==Solution NMR Structure Of The Partially Activated MTS Deleted Form MinE Protein (delta10-ngMinE) From Neisseria gonorrheae==
-	<StructureSection load='6u6q' size='340' side='right'caption='[[6u6q]]' scene=''>	+	<StructureSection load='6u6q' size='340' side='right'caption='[[6u6q]], [[NMR_Ensembles_of_Models \| 10 NMR models]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full ~~crystallographic~~ information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U6Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U6Q FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u6q]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"diplococcus_gonorrhoeae"_(zopf_1885)_lehmann_and_neumann_1896 "diplococcus gonorrhoeae" (zopf 1885) lehmann and neumann 1896]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U6Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U6Q FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u6q OCA], [http://pdbe.org/6u6q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u6q RCSB], [http://www.ebi.ac.uk/pdbsum/6u6q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u6q ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">minE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=485 "Diplococcus gonorrhoeae" (Zopf 1885) Lehmann and Neumann 1896])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u6q OCA], [http://pdbe.org/6u6q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u6q RCSB], [http://www.ebi.ac.uk/pdbsum/6u6q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u6q ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MINE_NEIGO MINE_NEIGO]] Prevents the cell division inhibition by proteins MinC and MinD at internal division sites while permitting inhibition at polar sites. This ensures cell division at the proper site by restricting the formation of a division septum at the midpoint of the long axis of the cell (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacterial MinD and MinE form a standing oscillatory wave which positions the cell division inhibitor MinC, that binds MinD, everywhere on the membrane except at the midpoint of the cell, ensuring midcell positioning of the cytokinetic septum. During this process MinE undergoes fold switching as it interacts with different partners. We explore the exchange dynamics between major and excited states of the MinE dimer in 3 forms using (15)N relaxation dispersion NMR: the full-length protein (6-stranded beta-sheet sandwiched between 4 helices) representing the resting state; a 10-residue N-terminal deletion (Delta10) mimicking the membrane-binding competent state where the N-terminal helix is detached to interact with membrane; and N-terminal deletions of either 30 (Delta30) or 10 residues with an I24N mutation (Delta10/I24N), in which the beta1-strands at the dimer interface are extruded and available to bind MinD, leaving behind a 4-stranded beta-sheet. Full-length MinE samples 2 "excited" states: The first is similar to a full-length/Delta10 heterodimer; the second, also sampled by Delta10, is either similar to or well along the pathway toward the 4-stranded beta-sheet form. Both Delta30 and Delta10/I24N sample 2 excited species: The first may involve destabilization of the beta3- and beta3'-strands at the dimer interface; changes in the second are more extensive, involving further disruption of secondary structure, possibly representing an ensemble of states on the pathway toward restoration of the resting state. The quantitative information on MinE conformational dynamics involving these excited states is crucial for understanding the oscillation pattern self-organization by MinD-MinE interaction dynamics on the membrane.
		+
		+	Probing transient excited states of the bacterial cell division regulator MinE by relaxation dispersion NMR spectroscopy.,Cai M, Huang Y, Shen Y, Li M, Mizuuchi M, Ghirlando R, Mizuuchi K, Clore GM Proc Natl Acad Sci U S A. 2019 Dec 17;116(51):25446-25455. doi:, 10.1073/pnas.1915948116. Epub 2019 Nov 26. PMID:31772021<ref>PMID:31772021</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6u6q" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cai M]]	+	[[Category: Cai, M]]
-	[[Category: Clore M]]	+	[[Category: Clore, M]]
-	[[Category: Shen Y]]	+	[[Category: Shen, Y]]
		+	[[Category: Cell cycle]]
		+	[[Category: Cell division]]
		+	[[Category: Mind]]
		+	[[Category: Mine]]

OCA at 14:06, 8 July 2020

OCA — Wed, 08 Jul 2020 14:06:58 GMT

←Older revision		Revision as of 14:06, 8 July 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6u6q is ON HOLD until Paper Publication~~	+	==Solution NMR Structure Of The Partially Activated MTS Deleted Form MinE Protein (delta10-ngMinE) From Neisseria gonorrheae==
-		+	<StructureSection load='6u6q' size='340' side='right'caption='[[6u6q]]' scene=''>
-	~~Authors: Cai, M., Shen, Y., Clore, M.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U6Q OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U6Q FirstGlance]. <br>
-	~~Description:~~ Solution NMR Structure Of The Partially Activated MTS Deleted Form MinE Protein (delta10-ngMinE) From Neisseria gonorrheae	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u6q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u6q OCA], [http://pdbe.org/6u6q PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u6q RCSB], [http://www.ebi.ac.uk/pdbsum/6u6q PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u6q ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Cai, M]]	+	__TOC__
-	[[Category: Clore, M]]	+	</StructureSection>
-	[[Category: Shen, Y]]	+	[[Category: Large Structures]]
		+	[[Category: Cai M]]
		+	[[Category: Clore M]]
		+	[[Category: Shen Y]]

OCA at 13:45, 20 November 2019

OCA — Wed, 20 Nov 2019 13:45:01 GMT

←Older revision		Revision as of 13:45, 20 November 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6u6q is ON HOLD	+	The entry 6u6q is ON HOLD until Paper Publication

	Authors: Cai, M., Shen, Y., Clore, M.		Authors: Cai, M., Shen, Y., Clore, M.

OCA: Protected "6u6q" [edit=sysop:move=sysop]

OCA — Wed, 02 Oct 2019 08:18:27 GMT

Protected "6u6q" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:18, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6u6q is ON HOLD Authors: Cai, M., Shen, Y., Clore, M. Description: Solution NMR Structure Of The Partially Activated MTS Deleted Form MinE Protein ...

OCA — Wed, 02 Oct 2019 08:18:25 GMT

New page: '''Unreleased structure''' The entry 6u6q is ON HOLD Authors: Cai, M., Shen, Y., Clore, M. Description: Solution NMR Structure Of The Partially Activated MTS Deleted Form MinE Protein ...

New page

'''Unreleased structure'''

The entry 6u6q is ON HOLD

Authors: Cai, M., Shen, Y., Clore, M.

Description: Solution NMR Structure Of The Partially Activated MTS Deleted Form MinE Protein (delta10-ngMinE) From Neisseria gonorrheae
[[Category: Unreleased Structures]]
[[Category: Cai, M]]
[[Category: Clore, M]]
[[Category: Shen, Y]]