6u9p - Revision history

OCA at 07:47, 11 October 2023

OCA — Wed, 11 Oct 2023 07:47:15 GMT

←Older revision		Revision as of 07:47, 11 October 2023
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	<StructureSection load='6u9p' size='340' side='right'caption='[[6u9p]], [[Resolution\|resolution]] 1.65Å' scene=''>		<StructureSection load='6u9p' size='340' side='right'caption='[[6u9p]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6u9p]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"methanobacterium_thermoautotrophicus"_(sic)_zeikus_and_wolfe_1972 "methanobacterium thermoautotrophicus" (sic) zeikus and wolfe 1972~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U9P OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6U9P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u9p]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Methanothermobacter_thermautotrophicus Methanothermobacter thermautotrophicus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U9P OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6U9P FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=HEZ:HEXANE~~-1~~,6-DIOL'>HEZ</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.65Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~mthK, MTH_1520 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=145262 "Methanobacterium thermoautotrophicus" (sic) Zeikus and Wolfe 1972])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6u9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u9p OCA], [~~http~~://pdbe.org/6u9p PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6u9p RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6u9p PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6u9p ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6u9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u9p OCA], [https://pdbe.org/6u9p PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6u9p RCSB], [https://www.ebi.ac.uk/pdbsum/6u9p PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6u9p ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/MTHK_METTH MTHK_METTH]] Calcium-gated potassium channel.	+	[https://www.uniprot.org/uniprot/MTHK_METTH MTHK_METTH] Calcium-gated potassium channel.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</div>		</div>
	<div class="pdbe-citations 6u9p" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6u9p" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Potassium channel 3D structures\|Potassium channel 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Nimigean, C M~~]]	+	[[Category: Methanothermobacter thermautotrophicus]]
-	[[Category: ~~Posson, D J~~]]	+	[[Category: Nimigean CM]]
-	[[Category: ~~Potassium ion channel mthk]]~~	+	[[Category: Posson DJ]]
-	~~[[Category: Transport protein~~]]	+

OCA at 09:51, 18 November 2020

OCA — Wed, 18 Nov 2020 09:51:12 GMT

←Older revision		Revision as of 09:51, 18 November 2020
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	==Wild-type MthK pore in ~150 mM K+==		==Wild-type MthK pore in ~150 mM K+==
-	<StructureSection load='6u9p' size='340' side='right'caption='[[6u9p]]' scene=''>	+	<StructureSection load='6u9p' size='340' side='right'caption='[[6u9p]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U9P OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U9P FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6u9p]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/"methanobacterium_thermoautotrophicus"_(sic)_zeikus_and_wolfe_1972 "methanobacterium thermoautotrophicus" (sic) zeikus and wolfe 1972]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U9P OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U9P FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u9p OCA], [http://pdbe.org/6u9p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u9p RCSB], [http://www.ebi.ac.uk/pdbsum/6u9p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u9p ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=HEZ:HEXANE-1,6-DIOL'>HEZ</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">mthK, MTH_1520 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=145262 "Methanobacterium thermoautotrophicus" (sic) Zeikus and Wolfe 1972])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u9p OCA], [http://pdbe.org/6u9p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u9p RCSB], [http://www.ebi.ac.uk/pdbsum/6u9p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u9p ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/MTHK_METTH MTHK_METTH]] Calcium-gated potassium channel.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Potassium channels can become nonconducting via inactivation at a gate inside the highly conserved selectivity filter (SF) region near the extracellular side of the membrane. In certain ligand-gated channels, such as BK channels and MthK, a Ca(2+)-activated K(+) channel from Methanobacterium thermoautotrophicum, the SF has been proposed to play a role in opening and closing rather than inactivation, although the underlying conformational changes are unknown. Using X-ray crystallography, identical conductive MthK structures were obtained in wide-ranging K(+) concentrations (6 to 150 mM), unlike KcsA, whose SF collapses at low permeant ion concentrations. Surprisingly, three of the SF's four binding sites remained almost fully occupied throughout this range, indicating high affinities (likely submillimolar), while only the central S2 site titrated, losing its ion at 6 mM, indicating low K(+) affinity ( approximately 50 mM). Molecular simulations showed that the MthK SF can also collapse in the absence of K(+), similar to KcsA, but that even a single K(+) binding at any of the SF sites, except S4, can rescue the conductive state. The uneven titration across binding sites differs from KcsA, where SF sites display a uniform decrease in occupancy with K(+) concentration, in the low millimolar range, leading to SF collapse. We found that ions were disfavored in MthK's S2 site due to weaker coordination by carbonyl groups, arising from different interactions with the pore helix and water behind the SF. We conclude that these differences in interactions endow the seemingly identical SFs of KcsA and MthK with strikingly different inactivating phenotypes.
		+
		+	Selectivity filter ion binding affinity determines inactivation in a potassium channel.,Boiteux C, Posson DJ, Allen TW, Nimigean CM Proc Natl Acad Sci U S A. 2020 Nov 5. pii: 2009624117. doi:, 10.1073/pnas.2009624117. PMID:33154158<ref>PMID:33154158</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6u9p" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Nimigean CM]]	+	[[Category: Nimigean, C M]]
-	[[Category: Posson DJ]]	+	[[Category: Posson, D J]]
		+	[[Category: Potassium ion channel mthk]]
		+	[[Category: Transport protein]]

OCA at 07:21, 4 November 2020

OCA — Wed, 04 Nov 2020 07:21:47 GMT

←Older revision		Revision as of 07:21, 4 November 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6u9p ~~is ON HOLD until Paper Publication~~	+	==Wild-type MthK pore in ~150 mM K+==
-		+	<StructureSection load='6u9p' size='340' side='right'caption='[[6u9p]]' scene=''>
-	~~Authors~~: ~~Posson~~, D.J., ~~Nimigean~~, C.M.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6U9P OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6U9P FirstGlance]. <br>
-	~~Description~~: ~~Wild~~-~~type MthK pore in ~150 mM K+~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6u9p FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6u9p OCA], [http://pdbe.org/6u9p PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6u9p RCSB], [http://www.ebi.ac.uk/pdbsum/6u9p PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6u9p ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Nimigean~~, C.M~~]]	+	__TOC__
-	[[Category: Posson~~, D.J~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Nimigean CM]]
		+	[[Category: Posson DJ]]

OCA: Protected "6u9p" [edit=sysop:move=sysop]

OCA — Wed, 02 Oct 2019 08:27:14 GMT

Protected "6u9p" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:27, 2 October 2019

OCA: New page: '''Unreleased structure''' The entry 6u9p is ON HOLD until Paper Publication Authors: Posson, D.J., Nimigean, C.M. Description: Wild-type MthK pore in ~150 mM K+ [[Category: Unreleased...

OCA — Wed, 02 Oct 2019 08:27:12 GMT

New page: '''Unreleased structure''' The entry 6u9p is ON HOLD until Paper Publication Authors: Posson, D.J., Nimigean, C.M. Description: Wild-type MthK pore in ~150 mM K+ [[Category: Unreleased...

New page

'''Unreleased structure'''

The entry 6u9p is ON HOLD until Paper Publication

Authors: Posson, D.J., Nimigean, C.M.

Description: Wild-type MthK pore in ~150 mM K+
[[Category: Unreleased Structures]]
[[Category: Nimigean, C.M]]
[[Category: Posson, D.J]]