6uiy - Revision history

OCA at 07:52, 11 October 2023

OCA — Wed, 11 Oct 2023 07:52:19 GMT

←Older revision		Revision as of 07:52, 11 October 2023
Line 3:		Line 3:
	<StructureSection load='6uiy' size='340' side='right'caption='[[6uiy]], [[Resolution\|resolution]] 1.47Å' scene=''>		<StructureSection load='6uiy' size='340' side='right'caption='[[6uiy]], [[Resolution\|resolution]] 1.47Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6uiy]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~As_4.1583 As 4.1583~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UIY OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6UIY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6uiy]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UIY OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UIY FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=QG1:{5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-N-(2-{[(pyridin-2-yl)methyl][(pyridin-2-yl-kappaN)methyl]amino-kappaN}ethyl)pentanamide}iron(2+)'>QG1</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.47Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6uiy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uiy OCA], [~~http~~://pdbe.org/6uiy PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6uiy RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6uiy PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6uiy ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=QG1:{5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-N-(2-{[(pyridin-2-yl)methyl][(pyridin-2-yl-kappaN)methyl]amino-kappaN}ethyl)pentanamide}iron(2+)'>QG1</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6uiy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uiy OCA], [https://pdbe.org/6uiy PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6uiy RCSB], [https://www.ebi.ac.uk/pdbsum/6uiy PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6uiy ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).	+	[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 22:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: As 4 1583]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Batyuk, A]]	+	[[Category: Streptomyces avidinii]]
-	[[Category: Bhowmick, A]]	+	[[Category: Batyuk A]]
-	[[Category: Borovik~~, A S~~]]	+	[[Category: Bhowmick A]]
-	[[Category: Brewster~~, A S~~]]	+	[[Category: Borovik AS]]
-	[[Category: Follmer~~, A H~~]]	+	[[Category: Brewster AS]]
-	[[Category: Fuller~~, F D~~]]	+	[[Category: Follmer AH]]
-	[[Category: Green~~, M T~~]]	+	[[Category: Fuller FD]]
-	[[Category: Gul, S]]	+	[[Category: Green MT]]
-	[[Category: Heinisch, T]]	+	[[Category: Gul S]]
-	[[Category: Kern, J]]	+	[[Category: Heinisch T]]
-	[[Category: Kim, I S]]	+	[[Category: Kern J]]
-	[[Category: Miller~~, K R~~]]	+	[[Category: Kim I-S]]
-	[[Category: Mittra, K]]	+	[[Category: Miller KR]]
-	[[Category: Paretsky~~, J D~~]]	+	[[Category: Mittra K]]
-	[[Category: Sauter~~, N K~~]]	+	[[Category: Paretsky JD]]
-	[[Category: Sutherlin~~, K D~~]]	+	[[Category: Sauter NK]]
-	[[Category: Ward~~, T R~~]]	+	[[Category: Sutherlin KD]]
-	[[Category: Yano, J]]	+	[[Category: Ward TR]]
-	~~[[Category: Biotin binding artificial metalloprotein]]~~	+	[[Category: Yano J]]
-	~~[[Category: Metal binding protein~~]]	+

OCA at 05:59, 13 May 2020

OCA — Wed, 13 May 2020 05:59:00 GMT

←Older revision		Revision as of 05:59, 13 May 2020
Line 1:		Line 1:

	==Artificial Iron Proteins: Modelling the Active Sites in Non-Heme Dioxygenases==		==Artificial Iron Proteins: Modelling the Active Sites in Non-Heme Dioxygenases==
-	<StructureSection load='6uiy' size='340' side='right'caption='[[6uiy]]' scene=''>	+	<StructureSection load='6uiy' size='340' side='right'caption='[[6uiy]], [[Resolution\|resolution]] 1.47Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UIY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UIY FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6uiy]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/As_4.1583 As 4.1583]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UIY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UIY FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6uiy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uiy OCA], [http://pdbe.org/6uiy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uiy RCSB], [http://www.ebi.ac.uk/pdbsum/6uiy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uiy ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=QG1:{5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]-N-(2-{[(pyridin-2-yl)methyl][(pyridin-2-yl-kappaN)methyl]amino-kappaN}ethyl)pentanamide}iron(2+)'>QG1</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6uiy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uiy OCA], [http://pdbe.org/6uiy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uiy RCSB], [http://www.ebi.ac.uk/pdbsum/6uiy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uiy ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV]] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	An important class of non-heme dioxygenases contains a conserved Fe binding site that consists of a 2-His-1-carboxylate facial triad. Results from structural biology show that, in the resting state, these proteins are six-coordinate with aqua ligands occupying the remaining three coordination sites. We have utilized biotin-streptavidin (Sav) technology to design new artificial Fe proteins (ArMs) that have many of the same structural features found within active sites of these non-heme dioxygenases. An Sav variant was isolated that contains the S112E mutation, which installed a carboxylate side chain in the appropriate position to bind to a synthetic Fe(II) complex confined within Sav. Structural studies using X-ray diffraction (XRD) methods revealed a facial triad binding site that is composed of two N donors from the biotinylated ligand and the monodentate coordination of the carboxylate from S112E. Two aqua ligands complete the primary coordination sphere of the Fe(II) center with both involved in hydrogen bond networks within Sav. The corresponding Fe(III) protein was also prepared and structurally characterized to show a six-coordinate complex with two exogenous acetato ligands. The Fe(III) protein was further shown to bind an exogenous azido ligand through replacement of one acetato ligand. Spectroscopic studies of the ArMs in solution support the results found by XRD.
		+
		+	Artificial Iron Proteins: Modeling the Active Sites in Non-Heme Dioxygenases.,Miller KR, Paretsky JD, Follmer AH, Heinisch T, Mittra K, Gul S, Kim IS, Fuller FD, Batyuk A, Sutherlin KD, Brewster AS, Bhowmick A, Sauter NK, Kern J, Yano J, Green MT, Ward TR, Borovik AS Inorg Chem. 2020 May 4;59(9):6000-6009. doi: 10.1021/acs.inorgchem.9b03791. Epub , 2020 Apr 20. PMID:32309932<ref>PMID:32309932</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6uiy" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: As 4 1583]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Batyuk A]]	+	[[Category: Batyuk, A]]
-	[[Category: Bhowmick A]]	+	[[Category: Bhowmick, A]]
-	[[Category: Borovik AS]]	+	[[Category: Borovik, A S]]
-	[[Category: Brewster AS]]	+	[[Category: Brewster, A S]]
-	[[Category: Follmer AH]]	+	[[Category: Follmer, A H]]
-	[[Category: Fuller FD]]	+	[[Category: Fuller, F D]]
-	[[Category: Green MT]]	+	[[Category: Green, M T]]
-	[[Category: Gul S]]	+	[[Category: Gul, S]]
-	[[Category: Heinisch T]]	+	[[Category: Heinisch, T]]
-	[[Category: Kern J]]	+	[[Category: Kern, J]]
-	[[Category: Kim I-S]]	+	[[Category: Kim, I S]]
-	[[Category: Miller KR]]	+	[[Category: Miller, K R]]
-	[[Category: Mittra K]]	+	[[Category: Mittra, K]]
-	[[Category: Paretsky JD]]	+	[[Category: Paretsky, J D]]
-	[[Category: Sauter NK]]	+	[[Category: Sauter, N K]]
-	[[Category: Sutherlin KD]]	+	[[Category: Sutherlin, K D]]
-	[[Category: Ward TR]]	+	[[Category: Ward, T R]]
-	[[Category: Yano J]]	+	[[Category: Yano, J]]
		+	[[Category: Biotin binding artificial metalloprotein]]
		+	[[Category: Metal binding protein]]

OCA at 06:04, 6 May 2020

OCA — Wed, 06 May 2020 06:04:34 GMT

←Older revision		Revision as of 06:04, 6 May 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6uiy is ~~ON HOLD until Paper Publication~~	+	==Artificial Iron Proteins: Modelling the Active Sites in Non-Heme Dioxygenases==
-		+	<StructureSection load='6uiy' size='340' side='right'caption='[[6uiy]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UIY OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UIY FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6uiy FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uiy OCA], [http://pdbe.org/6uiy PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uiy RCSB], [http://www.ebi.ac.uk/pdbsum/6uiy PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uiy ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Batyuk A]]
		+	[[Category: Bhowmick A]]
		+	[[Category: Borovik AS]]
		+	[[Category: Brewster AS]]
		+	[[Category: Follmer AH]]
		+	[[Category: Fuller FD]]
		+	[[Category: Green MT]]
		+	[[Category: Gul S]]
		+	[[Category: Heinisch T]]
		+	[[Category: Kern J]]
		+	[[Category: Kim I-S]]
		+	[[Category: Miller KR]]
		+	[[Category: Mittra K]]
		+	[[Category: Paretsky JD]]
		+	[[Category: Sauter NK]]
		+	[[Category: Sutherlin KD]]
		+	[[Category: Ward TR]]
		+	[[Category: Yano J]]

OCA at 09:31, 13 November 2019

OCA — Wed, 13 Nov 2019 09:31:05 GMT

←Older revision		Revision as of 09:31, 13 November 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6uiy is ON HOLD	+	The entry 6uiy is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6uiy" [edit=sysop:move=sysop]

OCA — Thu, 10 Oct 2019 04:01:16 GMT

Protected "6uiy" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:01, 10 October 2019

OCA: New page: '''Unreleased structure''' The entry 6uiy is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 10 Oct 2019 04:01:15 GMT

New page: '''Unreleased structure''' The entry 6uiy is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6uiy is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]