6un4 - Revision history

OCA at 07:54, 11 October 2023

OCA — Wed, 11 Oct 2023 07:54:49 GMT

←Older revision		Revision as of 07:54, 11 October 2023
Line 3:		Line 3:
	<StructureSection load='6un4' size='340' side='right'caption='[[6un4]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='6un4' size='340' side='right'caption='[[6un4]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6un4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Aeqvi Aeqvi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UN4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UN4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6un4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UN4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UN4 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.499Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=3CT:3-CHLORO-L-TYROSINE'>3CT</scene>, <scene name='pdbligand=OHD:'>OHD</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3CT:3-CHLORO-L-TYROSINE'>3CT</scene>, <scene name='pdbligand=OHD:{(4Z)-2-[(1S)-1-aminoethyl]-4-[(3-chloro-4-hydroxyphenyl)methylidene]-5-oxo-4,5-dihydro-1H-imidazol-1-yl}acetic+acid'>OHD</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">GFP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6un4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6un4 OCA], [https://pdbe.org/6un4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6un4 RCSB], [https://www.ebi.ac.uk/pdbsum/6un4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6un4 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6un4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6un4 OCA], [https://pdbe.org/6un4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6un4 RCSB], [https://www.ebi.ac.uk/pdbsum/6un4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6un4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.	+	[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Aeqvi~~]]	+	[[Category: Aequorea victoria]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Boxer~~, S G~~]]	+	[[Category: Boxer SG]]
-	[[Category: Chang, J]]	+	[[Category: Chang J]]
-	[[Category: Lin, C Y]]	+	[[Category: Lin C-Y]]
-	[[Category: Romei~~, M G]]~~	+	[[Category: Romei MG]]
-	~~[[Category: Fluorescent protein]]~~	+
-	~~[[Category: Photoswitchable fluorescent protein~~]]	+

OCA at 10:16, 13 April 2022

OCA — Wed, 13 Apr 2022 10:16:48 GMT

←Older revision		Revision as of 10:16, 13 April 2022
Line 3:		Line 3:
	<StructureSection load='6un4' size='340' side='right'caption='[[6un4]], [[Resolution\|resolution]] 1.50Å' scene=''>		<StructureSection load='6un4' size='340' side='right'caption='[[6un4]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6un4]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UN4 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6UN4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6un4]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UN4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UN4 FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat">~~<scene name='pdbligand=3CT:3-CHLORO-L-TYROSINE'>3CT</scene>,~~ <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
-	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OHD:'>OHD</scene></td></tr>	+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=3CT:3-CHLORO-L-TYROSINE'>3CT</scene>, <scene name='pdbligand=OHD:'>OHD</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GFP ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GFP ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6un4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6un4 OCA], [~~http~~://pdbe.org/6un4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6un4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6un4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6un4 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6un4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6un4 OCA], [https://pdbe.org/6un4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6un4 RCSB], [https://www.ebi.ac.uk/pdbsum/6un4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6un4 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.	+	[[https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==

OCA at 07:34, 4 November 2020

OCA — Wed, 04 Nov 2020 07:34:57 GMT

←Older revision		Revision as of 07:34, 4 November 2020
Line 1:		Line 1:

	==Crystal structure of rsEGFP2, Y67(3-ClY), Y107(3-ClY)==		==Crystal structure of rsEGFP2, Y67(3-ClY), Y107(3-ClY)==
-	<StructureSection load='6un4' size='340' side='right'caption='[[6un4]]' scene=''>	+	<StructureSection load='6un4' size='340' side='right'caption='[[6un4]], [[Resolution\|resolution]] 1.50Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UN4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UN4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6un4]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Aeqvi Aeqvi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UN4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UN4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6un4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6un4 OCA], [http://pdbe.org/6un4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6un4 RCSB], [http://www.ebi.ac.uk/pdbsum/6un4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6un4 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=3CT:3-CHLORO-L-TYROSINE'>3CT</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=OHD:'>OHD</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">GFP ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=6100 AEQVI])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6un4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6un4 OCA], [http://pdbe.org/6un4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6un4 RCSB], [http://www.ebi.ac.uk/pdbsum/6un4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6un4 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI]] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Photo-induced structural rearrangements of chromophore-containing proteins are essential for various light-dependent signaling pathways and optogenetic applications. Ultrafast structural and spectroscopic methods have offered insights into these structural rearrangements across many timescales. However, questions still remain about exact mechanistic details, especially regarding photoisomerization of the chromophore within these proteins femtoseconds to picoseconds after photoexcitation. Instrumentation advancements for time-resolved crystallography and ultrafast electron diffraction provide a promising opportunity to study these reactions, but achieving enough signal-to-noise is a constant challenge. Here we present four new photoactive yellow protein constructs and one new fluorescent protein construct that contain heavy atoms either within or around the chromophore and can be expressed with high yields. Structural characterization of these constructs, most at atomic resolution, show minimal perturbation caused by the heavy atoms compared to wild-type structures. Spectroscopic studies report the effects of the heavy atom identity and location on the chromophore's photophysical properties. None of the substitutions prevent photoisomerization, although certain rates within the photocycle may be affected. Overall, these new proteins containing heavy atoms are ideal samples for state-of-theart time-resolved crystallography and electron diffraction experiments to elucidate crucial mechanistic information of photoisomerization.
		+
		+	Structural and spectroscopic characterization of photoactive yellow protein and photoswitchable fluorescent protein constructs containing heavy atoms.,Romei MG, Lin CY, Boxer SG J Photochem Photobiol A Chem. 2020 Oct 1;401. doi:, 10.1016/j.jphotochem.2020.112738. Epub 2020 Jun 30. PMID:32753830<ref>PMID:32753830</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6un4" style="background-color:#fffaf0;"></div>

	==See Also==		==See Also==
	*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]		*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aeqvi]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Boxer SG]]	+	[[Category: Boxer, S G]]
-	[[Category: Chang J]]	+	[[Category: Chang, J]]
-	[[Category: Lin C-Y]]	+	[[Category: Lin, C Y]]
-	[[Category: Romei MG]]	+	[[Category: Romei, M G]]
		+	[[Category: Fluorescent protein]]
		+	[[Category: Photoswitchable fluorescent protein]]

OCA at 06:34, 19 August 2020

OCA — Wed, 19 Aug 2020 06:34:58 GMT

←Older revision		Revision as of 06:34, 19 August 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6un4 is ~~ON HOLD until Paper Publication~~	+	==Crystal structure of rsEGFP2, Y67(3-ClY), Y107(3-ClY)==
		+	<StructureSection load='6un4' size='340' side='right'caption='[[6un4]]' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UN4 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UN4 FirstGlance]. <br>
		+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6un4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6un4 OCA], [http://pdbe.org/6un4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6un4 RCSB], [http://www.ebi.ac.uk/pdbsum/6un4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6un4 ProSAT]</span></td></tr>
		+	</table>

-	~~Authors~~:	+	==See Also==
-		+	*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
-	~~Description~~:	+	__TOC__
-	[[Category: ~~Unreleased Structures~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Boxer SG]]
		+	[[Category: Chang J]]
		+	[[Category: Lin C-Y]]
		+	[[Category: Romei MG]]

OCA at 13:51, 20 November 2019

OCA — Wed, 20 Nov 2019 13:51:37 GMT

←Older revision		Revision as of 13:51, 20 November 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6un4 is ON HOLD	+	The entry 6un4 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6un4" [edit=sysop:move=sysop]

OCA — Wed, 16 Oct 2019 04:43:25 GMT

Protected "6un4" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:43, 16 October 2019

OCA: New page: '''Unreleased structure''' The entry 6un4 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 16 Oct 2019 04:43:23 GMT

New page: '''Unreleased structure''' The entry 6un4 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6un4 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]