6upn - Revision history

OCA at 09:29, 20 March 2024

OCA — Wed, 20 Mar 2024 09:29:55 GMT

←Older revision		Revision as of 09:29, 20 March 2024
Line 3:		Line 3:
	<StructureSection load='6upn' size='340' side='right'caption='[[6upn]], [[Resolution\|resolution]] 10.00Å' scene=''>		<StructureSection load='6upn' size='340' side='right'caption='[[6upn]], [[Resolution\|resolution]] 10.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6upn]] is a 48 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UPN OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6UPN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6upn]] is a 48 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UPN OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UPN FirstGlance]. <br>
-	</td></tr><tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~><div style='overflow: auto; max-height: 3em;'>[[6up6\|6up6]]</div></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 10Å</td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~">~~<b>~~[[~~Gene~~\|~~Gene:~~]]~~</b></td><td class="sblockDat">SH3GLB1, KIAA0491, CGI-61 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=9606 HUMAN])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6upn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6upn OCA], [https://pdbe.org/6upn PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6upn RCSB], [https://www.ebi.ac.uk/pdbsum/6upn PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6upn ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6upn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6upn OCA], [~~http~~://pdbe.org/6upn PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6upn RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6upn PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6upn ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/SHLB1_HUMAN SHLB1_HUMAN]] May be required for normal outer mitochondrial membrane dynamics (PubMed:15452144). Required for coatomer-mediated retrograde transport in certain cells (By similarity). May recruit other proteins to membranes with high curvature. May promote membrane fusion (PubMed:11604418). Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588). Isoform 1 acts proapoptotic in fibroblasts (By similarity). Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2) (PubMed:17891140). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation (PubMed:21068542). Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123). Isoform 2 acts antiapoptotic in neuronal cells; involved in maintenance of mitochondrial morphology and promotes neuronal viability (By similarity).[UniProtKB:Q9JK48]<ref>PMID:11604418</ref> <ref>PMID:15452144</ref> <ref>PMID:17891140</ref> <ref>PMID:20643123</ref> <ref>PMID:21068542</ref>	+	[https://www.uniprot.org/uniprot/SHLB1_HUMAN SHLB1_HUMAN] May be required for normal outer mitochondrial membrane dynamics (PubMed:15452144). Required for coatomer-mediated retrograde transport in certain cells (By similarity). May recruit other proteins to membranes with high curvature. May promote membrane fusion (PubMed:11604418). Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588). Isoform 1 acts proapoptotic in fibroblasts (By similarity). Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2) (PubMed:17891140). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation (PubMed:21068542). Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123). Isoform 2 acts antiapoptotic in neuronal cells; involved in maintenance of mitochondrial morphology and promotes neuronal viability (By similarity).[UniProtKB:Q9JK48]<ref>PMID:11604418</ref> <ref>PMID:15452144</ref> <ref>PMID:17891140</ref> <ref>PMID:20643123</ref> <ref>PMID:21068542</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Membrane remodeling is a common theme in a variety of cellular processes. Here, we investigated membrane remodeling N-BAR protein endophilin B1, a critical player in diverse intracellular trafficking events, including mitochondrial and Golgi fission, and apoptosis. We find that endophilin B1 assembles into helical scaffolds on membranes, and that both membrane binding and assembly are driven by interactions between N-terminal helix H0 and the lipid bilayer. Furthermore, we find that endophilin B1 membrane remodeling is auto-inhibited and identify direct SH3 domain-H0 interactions as the underlying mechanism. Our results indicate that lipid composition plays a role in dictating endophilin B1 activity. Taken together, this study provides insight into a poorly understood N-BAR protein family member and highlights molecular mechanisms that may be general for the regulation of membrane remodeling. Our work suggests that interplay between membrane lipids and membrane interacting proteins facilitates spatial and temporal coordination of membrane remodeling.	+
-		+
-	Amphipathic Motifs Regulate N-BAR Protein Endophilin B1 Auto-inhibition and Drive Membrane Remodeling.,Bhatt VS, Ashley R, Sundborger-Lunna A Structure. 2020 Oct 13. pii: S0969-2126(20)30370-1. doi:, 10.1016/j.str.2020.09.012. PMID:33086035<ref>PMID:33086035</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6upn" style="background-color:#fffaf0;"></div~~>	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bhatt~~, V S~~]]	+	[[Category: Bhatt VS]]
-	[[Category: Sundborger-Lunna~~, A C]]~~	+	[[Category: Sundborger-Lunna AC]]
-	~~[[Category: Amphipathic helix]]~~	+
-	~~[[Category: Bar protein]]~~	+
-	~~[[Category: Cell death]]~~	+
-	~~[[Category: Cytosolic protein]]~~	+
-	~~[[Category: Membrane binding]]~~	+
-	~~[[Category: Membrane trafficking]]~~	+
-	~~[[Category: Sh3 domain~~]]	+

OCA at 08:21, 20 January 2021

OCA — Wed, 20 Jan 2021 08:21:53 GMT

←Older revision		Revision as of 08:21, 20 January 2021
Line 4:		Line 4:
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6upn]] is a 48 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UPN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UPN FirstGlance]. <br>		<table><tr><td colspan='2'>[[6upn]] is a 48 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UPN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UPN FirstGlance]. <br>
-	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6up6\|6up6]]</td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6up6\|6up6]]</div></td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SH3GLB1, KIAA0491, CGI-61 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SH3GLB1, KIAA0491, CGI-61 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6upn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6upn OCA], [http://pdbe.org/6upn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6upn RCSB], [http://www.ebi.ac.uk/pdbsum/6upn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6upn ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6upn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6upn OCA], [http://pdbe.org/6upn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6upn RCSB], [http://www.ebi.ac.uk/pdbsum/6upn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6upn ProSAT]</span></td></tr>

OCA at 07:35, 4 November 2020

OCA — Wed, 04 Nov 2020 07:35:44 GMT

←Older revision		Revision as of 07:35, 4 November 2020
Line 1:		Line 1:

	==Endophilin B1 helical scaffold==		==Endophilin B1 helical scaffold==
-	<StructureSection load='6upn' size='340' side='right'caption='[[6upn]]' scene=''>	+	<StructureSection load='6upn' size='340' side='right'caption='[[6upn]], [[Resolution\|resolution]] 10.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UPN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UPN FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6upn]] is a 48 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UPN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UPN FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6upn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6upn OCA], [http://pdbe.org/6upn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6upn RCSB], [http://www.ebi.ac.uk/pdbsum/6upn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6upn ProSAT]</span></td></tr>	+	</td></tr><tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6up6\|6up6]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SH3GLB1, KIAA0491, CGI-61 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6upn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6upn OCA], [http://pdbe.org/6upn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6upn RCSB], [http://www.ebi.ac.uk/pdbsum/6upn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6upn ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SHLB1_HUMAN SHLB1_HUMAN]] May be required for normal outer mitochondrial membrane dynamics (PubMed:15452144). Required for coatomer-mediated retrograde transport in certain cells (By similarity). May recruit other proteins to membranes with high curvature. May promote membrane fusion (PubMed:11604418). Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed:16227588). Isoform 1 acts proapoptotic in fibroblasts (By similarity). Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2) (PubMed:17891140). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation (PubMed:21068542). Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123). Isoform 2 acts antiapoptotic in neuronal cells; involved in maintenance of mitochondrial morphology and promotes neuronal viability (By similarity).[UniProtKB:Q9JK48]<ref>PMID:11604418</ref> <ref>PMID:15452144</ref> <ref>PMID:17891140</ref> <ref>PMID:20643123</ref> <ref>PMID:21068542</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Membrane remodeling is a common theme in a variety of cellular processes. Here, we investigated membrane remodeling N-BAR protein endophilin B1, a critical player in diverse intracellular trafficking events, including mitochondrial and Golgi fission, and apoptosis. We find that endophilin B1 assembles into helical scaffolds on membranes, and that both membrane binding and assembly are driven by interactions between N-terminal helix H0 and the lipid bilayer. Furthermore, we find that endophilin B1 membrane remodeling is auto-inhibited and identify direct SH3 domain-H0 interactions as the underlying mechanism. Our results indicate that lipid composition plays a role in dictating endophilin B1 activity. Taken together, this study provides insight into a poorly understood N-BAR protein family member and highlights molecular mechanisms that may be general for the regulation of membrane remodeling. Our work suggests that interplay between membrane lipids and membrane interacting proteins facilitates spatial and temporal coordination of membrane remodeling.
		+
		+	Amphipathic Motifs Regulate N-BAR Protein Endophilin B1 Auto-inhibition and Drive Membrane Remodeling.,Bhatt VS, Ashley R, Sundborger-Lunna A Structure. 2020 Oct 13. pii: S0969-2126(20)30370-1. doi:, 10.1016/j.str.2020.09.012. PMID:33086035<ref>PMID:33086035</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6upn" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Bhatt VS]]	+	[[Category: Bhatt, V S]]
-	[[Category: Sundborger-Lunna AC]]	+	[[Category: Sundborger-Lunna, A C]]
		+	[[Category: Amphipathic helix]]
		+	[[Category: Bar protein]]
		+	[[Category: Cell death]]
		+	[[Category: Cytosolic protein]]
		+	[[Category: Membrane binding]]
		+	[[Category: Membrane trafficking]]
		+	[[Category: Sh3 domain]]

OCA at 05:46, 22 April 2020

OCA — Wed, 22 Apr 2020 05:46:28 GMT

←Older revision		Revision as of 05:46, 22 April 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6upn ~~is ON HOLD~~	+	==Endophilin B1 helical scaffold==
-		+	<StructureSection load='6upn' size='340' side='right'caption='[[6upn]]' scene=''>
-	~~Authors~~: ~~Bhatt, V~~.S., ~~Sundborger~~-~~Lunna~~, A.C.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UPN OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UPN FirstGlance]. <br>
-	~~Description: Endophilin B1 helical scaffold~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6upn FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6upn OCA], [http://pdbe.org/6upn PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6upn RCSB], [http://www.ebi.ac.uk/pdbsum/6upn PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6upn ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Sundborger-Lunna, A.C~~]]	+	__TOC__
-	[[Category: ~~Bhatt, V.S~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bhatt VS]]
		+	[[Category: Sundborger-Lunna AC]]

OCA: Protected "6upn" [edit=sysop:move=sysop]

OCA — Wed, 23 Oct 2019 05:20:20 GMT

Protected "6upn" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:20, 23 October 2019

OCA: New page: '''Unreleased structure''' The entry 6upn is ON HOLD Authors: Bhatt, V.S., Sundborger-Lunna, A.C. Description: Endophilin B1 helical scaffold Category: Unreleased Structures [[Cate...

OCA — Wed, 23 Oct 2019 05:20:18 GMT

New page: '''Unreleased structure''' The entry 6upn is ON HOLD Authors: Bhatt, V.S., Sundborger-Lunna, A.C. Description: Endophilin B1 helical scaffold Category: Unreleased Structures [[Cate...

New page

'''Unreleased structure'''

The entry 6upn is ON HOLD

Authors: Bhatt, V.S., Sundborger-Lunna, A.C.

Description: Endophilin B1 helical scaffold
[[Category: Unreleased Structures]]
[[Category: Sundborger-Lunna, A.C]]
[[Category: Bhatt, V.S]]