OCA at 07:56, 11 October 2023

OCA — Wed, 11 Oct 2023 07:56:09 GMT

←Older revision		Revision as of 07:56, 11 October 2023
Line 1:		Line 1:

-	====	+	==Crystal Structure of GTPase Domain of Human Septin 2 / Septin 8 Heterocomplex==
-	<StructureSection load='6upr' size='340' side='right'caption='[[6upr]]' scene=''>	+	<StructureSection load='6upr' size='340' side='right'caption='[[6upr]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6upr]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UPR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UPR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6upr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6upr OCA], [~~http~~://pdbe.org/6upr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6upr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6upr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6upr ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.299Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6upr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6upr OCA], [https://pdbe.org/6upr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6upr RCSB], [https://www.ebi.ac.uk/pdbsum/6upr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6upr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SEPT2_HUMAN SEPT2_HUMAN] Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Plays a role in the biogenesis of polarized columnar-shaped epithelium by maintaining polyglutamylated microtubules, thus facilitating efficient vesicle transport, and by impeding MAP4 binding to tubulin. Required for the progression through mitosis. Forms a scaffold at the midplane of the mitotic splindle required to maintain CENPE localization at kinetochores and consequently chromosome congression. During anaphase, may be required for chromosome segregation and spindle elongation. Plays a role in ciliogenesis and collective cell movements. In cilia, required for the integrity of the diffusion barrier at the base of the primary cilium that prevents diffusion of transmembrane proteins between the cilia and plasma membranes: probably acts by regulating the assembly of the tectonic-like complex (also named B9 complex) by localizing TMEM231 protein. May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.<ref>PMID:15774761</ref> <ref>PMID:17803907</ref> <ref>PMID:18209106</ref> <ref>PMID:19145258</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The assembly of a septin filament requires that homologous monomers must distinguish between one another in establishing appropriate interfaces with their neighbors. To understand this phenomenon at the molecular level, we present the first four crystal structures of heterodimeric septin complexes. We describe in detail the two distinct types of G-interface present within the octameric particles, which must polymerize to form filaments. These are formed between SEPT2 and SEPT6 and between SEPT7 and SEPT3, and their description permits an understanding of the structural basis for the selectivity necessary for correct filament assembly. By replacing SEPT6 by SEPT8 or SEPT11, it is possible to rationalize Kinoshita'spostulate, which predicts the exchangeability of septins from within a subgroup. Switches I and II, which in classical small GTPases provide a mechanism for nucleotide-dependent conformational change, have been repurposed in septins to play a fundamental role in molecular recognition. Specifically, it is switch I which holds the key to discriminating between the two different G-interfaces. Moreover, residues which are characteristic for a given subgroup play subtle, but pivotal, roles in guaranteeing that the correct interfaces are formed.
		+
		+	Molecular Recognition at Septin Interfaces: The Switches Hold the Key.,Rosa HVD, Leonardo DA, Brognara G, Brandao-Neto J, D'Muniz Pereira H, Araujo APU, Garratt RC J Mol Biol. 2020 Sep 7. pii: S0022-2836(20)30531-3. doi:, 10.1016/j.jmb.2020.09.001. PMID:32910969<ref>PMID:32910969</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6upr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Z-~~disk~~]]	+	[[Category: Araujo APU]]
		+	[[Category: Brandao-Neto J]]
		+	[[Category: Garratt RC]]
		+	[[Category: Leonardo DA]]
		+	[[Category: Pereira HM]]

OCA at 11:11, 23 September 2020

OCA — Wed, 23 Sep 2020 11:11:00 GMT

←Older revision		Revision as of 11:11, 23 September 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6upr ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6upr' size='340' side='right'caption='[[6upr]]' scene=''>
-	~~Authors~~: ~~Leonardo, D~~.A.~~, Pereira, H~~.~~M., Brandao~~-~~Neto, J~~.~~, Araujo, A~~.P.~~U., Garratt, R.C~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Crystal Structure of GTPase Domain of Human Septin 2~~ / ~~Septin 8 Heterocomplex~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6upr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6upr OCA], [http://pdbe.org/6upr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6upr RCSB], [http://www.ebi.ac.uk/pdbsum/6upr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6upr ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Brandao~~-~~Neto, J~~]]	+	__TOC__
-	[~~[Category~~: ~~Garratt~~, R.C]]	+	</StructureSection>
-	[~~[Category~~: ~~Pereira~~, H.M]]	+	[[Category: Large Structures]]
-	[[Category: ~~Araujo, A.P.U~~]]	+	[[Category: Z-disk]]
-	[[Category: ~~Leonardo, D.A~~]]	+

OCA: Protected "6upr" [edit=sysop:move=sysop]

OCA — Wed, 30 Oct 2019 07:38:44 GMT

Protected "6upr" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:38, 30 October 2019

OCA: New page: '''Unreleased structure''' The entry 6upr is ON HOLD until Paper Publication Authors: Leonardo, D.A., Pereira, H.M., Brandao-Neto, J., Araujo, A.P.U., Garratt, R.C. Description: Crysta...

OCA — Wed, 30 Oct 2019 07:38:42 GMT

New page: '''Unreleased structure''' The entry 6upr is ON HOLD until Paper Publication Authors: Leonardo, D.A., Pereira, H.M., Brandao-Neto, J., Araujo, A.P.U., Garratt, R.C. Description: Crysta...

New page

'''Unreleased structure'''

The entry 6upr is ON HOLD until Paper Publication

Authors: Leonardo, D.A., Pereira, H.M., Brandao-Neto, J., Araujo, A.P.U., Garratt, R.C.

Description: Crystal Structure of GTPase Domain of Human Septin 2 / Septin 8 Heterocomplex
[[Category: Unreleased Structures]]
[[Category: Brandao-Neto, J]]
[[Category: Garratt, R.C]]
[[Category: Pereira, H.M]]
[[Category: Araujo, A.P.U]]
[[Category: Leonardo, D.A]]

6upr - Revision history

OCA at 07:56, 11 October 2023

OCA at 11:11, 23 September 2020

OCA: Protected "6upr" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6upr is ON HOLD until Paper Publication Authors: Leonardo, D.A., Pereira, H.M., Brandao-Neto, J., Araujo, A.P.U., Garratt, R.C. Description: Crysta...