6uqz - Revision history

OCA at 07:56, 11 October 2023

OCA — Wed, 11 Oct 2023 07:56:43 GMT

←Older revision		Revision as of 07:56, 11 October 2023
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	<StructureSection load='6uqz' size='340' side='right'caption='[[6uqz]], [[Resolution\|resolution]] 1.43Å' scene=''>		<StructureSection load='6uqz' size='340' side='right'caption='[[6uqz]], [[Resolution\|resolution]] 1.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6uqz]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Pseae Pseae~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UQZ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6UQZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6uqz]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa_PAO1 Pseudomonas aeruginosa PAO1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UQZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UQZ FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>~~, ~~<scene name='pdbligand=ETM:2-(TRIMETHYLAMMONIUM)ETHYL+THIOL'>ETM</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.43Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~choE, PA4921 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode~~=~~Info&srchmode~~=~~5&id=208964 PSEAE]~~)</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ETM:2-(TRIMETHYLAMMONIUM)ETHYL+THIOL'>ETM</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6uqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uqz OCA], [~~http~~://pdbe.org/6uqz PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6uqz RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6uqz PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6uqz ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6uqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uqz OCA], [https://pdbe.org/6uqz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6uqz RCSB], [https://www.ebi.ac.uk/pdbsum/6uqz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6uqz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/Q9HUP2_PSEAE Q9HUP2_PSEAE]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Pseae~~]]	+	[[Category: Pseudomonas aeruginosa PAO1]]
-	[[Category: Pham~~, V D~~]]	+	[[Category: Pham VD]]
-	[[Category: Shi, R]]	+	[[Category: Shi R]]
-	~~[[Category: Esterase]]~~	+
-	~~[[Category: Hydrolase~~]]	+

OCA at 14:38, 8 July 2020

OCA — Wed, 08 Jul 2020 14:38:08 GMT

←Older revision		Revision as of 14:38, 8 July 2020
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	<StructureSection load='6uqz' size='340' side='right'caption='[[6uqz]], [[Resolution\|resolution]] 1.43Å' scene=''>		<StructureSection load='6uqz' size='340' side='right'caption='[[6uqz]], [[Resolution\|resolution]] 1.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6uqz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UQZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UQZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6uqz]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Pseae Pseae]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UQZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UQZ FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ETM:2-(TRIMETHYLAMMONIUM)ETHYL+THIOL'>ETM</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ETM:2-(TRIMETHYLAMMONIUM)ETHYL+THIOL'>ETM</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">choE, PA4921 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=208964 PSEAE])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6uqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uqz OCA], [http://pdbe.org/6uqz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uqz RCSB], [http://www.ebi.ac.uk/pdbsum/6uqz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uqz ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6uqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uqz OCA], [http://pdbe.org/6uqz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uqz RCSB], [http://www.ebi.ac.uk/pdbsum/6uqz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uqz ProSAT]</span></td></tr>
	</table>		</table>
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	</div>		</div>
	<div class="pdbe-citations 6uqz" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6uqz" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Acetylcholinesterase 3D structures\|Acetylcholinesterase 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
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	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Pseae]]
	[[Category: Pham, V D]]		[[Category: Pham, V D]]
	[[Category: Shi, R]]		[[Category: Shi, R]]
	[[Category: Esterase]]		[[Category: Esterase]]
	[[Category: Hydrolase]]		[[Category: Hydrolase]]

OCA at 06:09, 20 May 2020

OCA — Wed, 20 May 2020 06:09:52 GMT

←Older revision		Revision as of 06:09, 20 May 2020
Line 1:		Line 1:

	==Crystal structure of ChoE D285N mutant in complex with acetate and thiocholine==		==Crystal structure of ChoE D285N mutant in complex with acetate and thiocholine==
-	<StructureSection load='6uqz' size='340' side='right'caption='[[6uqz]]' scene=''>	+	<StructureSection load='6uqz' size='340' side='right'caption='[[6uqz]], [[Resolution\|resolution]] 1.43Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UQZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UQZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6uqz]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UQZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UQZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6uqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uqz OCA], [http://pdbe.org/6uqz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uqz RCSB], [http://www.ebi.ac.uk/pdbsum/6uqz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uqz ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=ETM:2-(TRIMETHYLAMMONIUM)ETHYL+THIOL'>ETM</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6uqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uqz OCA], [http://pdbe.org/6uqz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uqz RCSB], [http://www.ebi.ac.uk/pdbsum/6uqz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uqz ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mammalian acetylcholinesterase (AChE) is well studied, being important in both cholinergic brain synapses and the peripheral nervous systems and also a key drug target for many diseases. In contrast, little is known about the structures and molecular mechanism of prokaryotic acetylcholinesterases. We report here the structural and biochemical characterization of ChoE, a putative bacterial acetylcholinesterase from Pseudomonas aeruginosa. Analysis of wild-type (WT) and mutant strains indicated that ChoE is indispensable for P. aeruginosa growth with acetylcholine as the sole carbon and nitrogen source. The crystal structure of ChoE at 1.35 A resolution revealed that this enzyme adopts a typical fold of the SGNH hydrolase family. Although ChoE and eukaryotic AChEs catalyze the same reaction, their overall structures bear no similarities constituting an interesting example of convergent evolution. Among Ser-38, Asp-285, and His-288 of the catalytic triad residues, only Asp-285 was not essential for ChoE activity. Combined with kinetic analyses of WT and mutant proteins, multiple crystal structures of ChoE complexed with substrates, products, or reaction intermediate revealed the structural determinants for substrate recognition, snapshots of the various catalytic steps, and the molecular basis of substrate inhibition at high substrate concentrations. Our results indicate that substrate inhibition in ChoE is due to acetate release being blocked by the binding of a substrate molecule in a nonproductive mode. Owing to the distinct overall folds and significant differences of the active site between ChoE and eukaryotic AChEs, these structures will serve as a prototype for other prokaryotic acetylcholinesterases.
		+
		+	Structural insights into the putative bacterial acetylcholinesterase ChoE and its substrate inhibition mechanism.,Pham VD, To TA, Gagne-Thivierge C, Couture M, Lague P, Yao D, Picard ME, Lortie LA, Attere SA, Zhu X, Levesque RC, Charette SJ, Shi R J Biol Chem. 2020 May 5. pii: RA119.011809. doi: 10.1074/jbc.RA119.011809. PMID:32371400<ref>PMID:32371400</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6uqz" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Pham VD]]	+	[[Category: Pham, V D]]
-	[[Category: Shi R]]	+	[[Category: Shi, R]]
		+	[[Category: Esterase]]
		+	[[Category: Hydrolase]]

OCA at 05:33, 13 May 2020

OCA — Wed, 13 May 2020 05:33:50 GMT

←Older revision		Revision as of 05:33, 13 May 2020
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-	'''Unreleased structure'''

-	~~The entry 6uqz is ON HOLD until Paper Publication~~	+	==Crystal structure of ChoE D285N mutant in complex with acetate and thiocholine==
-		+	<StructureSection load='6uqz' size='340' side='right'caption='[[6uqz]]' scene=''>
-	~~Authors: Pham, V.D., Shi, R.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UQZ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UQZ FirstGlance]. <br>
-	~~Description:~~ Crystal structure of ChoE D285N mutant in complex with acetate and thiocholine	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6uqz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uqz OCA], [http://pdbe.org/6uqz PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uqz RCSB], [http://www.ebi.ac.uk/pdbsum/6uqz PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uqz ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: Pham~~, V.D~~]]	+	__TOC__
-	[[Category: Shi, R]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Pham VD]]
		+	[[Category: Shi R]]

OCA at 07:23, 1 April 2020

OCA — Wed, 01 Apr 2020 07:23:39 GMT

←Older revision		Revision as of 07:23, 1 April 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6uqz is ON HOLD until ~~Oct 21 2021~~	+	The entry 6uqz is ON HOLD until Paper Publication

	Authors: Pham, V.D., Shi, R.		Authors: Pham, V.D., Shi, R.

OCA at 09:32, 13 November 2019

OCA — Wed, 13 Nov 2019 09:32:32 GMT

←Older revision		Revision as of 09:32, 13 November 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6uqz is ON HOLD	+	The entry 6uqz is ON HOLD until Oct 21 2021

	Authors: Pham, V.D., Shi, R.		Authors: Pham, V.D., Shi, R.

OCA: Protected "6uqz" [edit=sysop:move=sysop]

OCA — Wed, 30 Oct 2019 07:41:35 GMT

Protected "6uqz" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:41, 30 October 2019

OCA: New page: '''Unreleased structure''' The entry 6uqz is ON HOLD Authors: Pham, V.D., Shi, R. Description: Crystal structure of ChoE D285N mutant in complex with acetate and thiocholine [[Category...

OCA — Wed, 30 Oct 2019 07:41:34 GMT

New page: '''Unreleased structure''' The entry 6uqz is ON HOLD Authors: Pham, V.D., Shi, R. Description: Crystal structure of ChoE D285N mutant in complex with acetate and thiocholine [[Category...

New page

'''Unreleased structure'''

The entry 6uqz is ON HOLD

Authors: Pham, V.D., Shi, R.

Description: Crystal structure of ChoE D285N mutant in complex with acetate and thiocholine
[[Category: Unreleased Structures]]
[[Category: Pham, V.D]]
[[Category: Shi, R]]