6uro - Revision history

OCA at 09:30, 20 March 2024

OCA — Wed, 20 Mar 2024 09:30:03 GMT

←Older revision		Revision as of 09:30, 20 March 2024
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	<SX load='6uro' size='340' side='right' viewer='molstar' caption='[[6uro]], [[Resolution\|resolution]] 3.60Å' scene=''>		<SX load='6uro' size='340' side='right' viewer='molstar' caption='[[6uro]], [[Resolution\|resolution]] 3.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6uro]] is a 6 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6URO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6URO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6uro]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Macaca_mulatta_polyomavirus_1 Macaca mulatta polyomavirus 1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6URO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6URO FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.6Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat"~~>[[6urg\|6urg]]</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	~~<tr~~ id~~='gene'><td class~~="~~sblockLbl~~"><~~b>[[Gene\|Gene~~:~~]]</b~~></td><td class="sblockDat">CPSF1, CPSF160 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), WDR33, WDC146 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CPSF4, CPSF30, NAR, NEB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CSTF3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6uro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uro OCA], [https://pdbe.org/6uro PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6uro RCSB], [https://www.ebi.ac.uk/pdbsum/6uro PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6uro ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6uro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uro OCA], [~~http~~://pdbe.org/6uro PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6uro RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6uro PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6uro ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CPSF1_HUMAN CPSF1_HUMAN]] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.<ref>PMID:14749727</ref> [[http://www.uniprot.org/uniprot/CPSF4_HUMAN CPSF4_HUMAN]] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U).<ref>PMID:9224719</ref> <ref>PMID:14749727</ref> [[http://www.uniprot.org/uniprot/WDR33_HUMAN WDR33_HUMAN]] Essential for both cleavage and polyadenylation of pre-mRNA 3' ends.<ref>PMID:19217410</ref> [[http://www.uniprot.org/uniprot/CSTF3_HUMAN CSTF3_HUMAN]] One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs.	+	[https://www.uniprot.org/uniprot/CPSF1_HUMAN CPSF1_HUMAN] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.<ref>PMID:14749727</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	The mammalian pre-mRNA 3'-end-processing machinery consists of cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF), and other proteins, but the overall architecture of this machinery remains unclear. CPSF contains two functionally distinct modules: a cleavage factor (mCF) and a polyadenylation specificity factor (mPSF). Here, we have produced recombinant human CPSF and CstF and examined these factors by electron microscopy (EM). We find that mPSF is the organizational core of the machinery, while the conformations of mCF and CstF and the position of mCF relative to mPSF are highly variable. We have identified by cryo-EM a segment in CPSF100 that tethers mCF to mPSF, and we have named it the PSF interaction motif (PIM). Mutations in the PIM can abolish CPSF formation, indicating that it is a crucial contact in CPSF. We have also obtained reconstructions of mCF and CstF77 by cryo-EM, assembled around the mPSF core.	+
-		+
-	Structural Insights into the Human Pre-mRNA 3'-End Processing Machinery.,Zhang Y, Sun Y, Shi Y, Walz T, Tong L Mol Cell. 2019 Nov 25. pii: S1097-2765(19)30834-2. doi:, 10.1016/j.molcel.2019.11.005. PMID:31810758<ref>PMID:31810758</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6uro" style="background-color:#fffaf0;"></div~~>	+

	==See Also==		==See Also==
		+	*[[Poly(ADP-ribose) polymerase 3D structures\|Poly(ADP-ribose) polymerase 3D structures]]
	*[[WD-repeat protein 3D structures\|WD-repeat protein 3D structures]]		*[[WD-repeat protein 3D structures\|WD-repeat protein 3D structures]]
	== References ==		== References ==
Line 27:		Line 18:
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: ~~Human~~]]	+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Sun, Y]]	+	[[Category: Macaca mulatta polyomavirus 1]]
-	[[Category: Tong, L]]	+	[[Category: Sun Y]]
-	[[Category: Walz, T]]	+	[[Category: Tong L]]
-	[[Category: Zhang, Y]]	+	[[Category: Walz T]]
-	~~[[Category: Aauaaa polyadenylation signal]]~~	+	[[Category: Zhang Y]]
-	~~[[Category: Mpsf]]~~	+
-	~~[[Category: Pre-mrna 3'-end processing]]~~	+
-	~~[[Category: Rna binding]]~~	+
-	~~[[Category: Rna binding protein-rna complex~~]]	+

OCA at 06:54, 11 April 2020

OCA — Sat, 11 Apr 2020 06:54:44 GMT

←Older revision		Revision as of 06:54, 11 April 2020
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	<SX load='6uro' size='340' side='right' viewer='molstar' caption='[[6uro]], [[Resolution\|resolution]] 3.60Å' scene=''>		<SX load='6uro' size='340' side='right' viewer='molstar' caption='[[6uro]], [[Resolution\|resolution]] 3.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6uro]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6URO OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6URO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6uro]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6URO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6URO FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6urg\|6urg]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6urg\|6urg]]</td></tr>
	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CPSF1, CPSF160 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), WDR33, WDC146 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CPSF4, CPSF30, NAR, NEB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CSTF3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>		<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CPSF1, CPSF160 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), WDR33, WDC146 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CPSF4, CPSF30, NAR, NEB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CSTF3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6uro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uro OCA], [http://pdbe.org/6uro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uro RCSB], [http://www.ebi.ac.uk/pdbsum/6uro PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uro ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6uro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uro OCA], [http://pdbe.org/6uro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uro RCSB], [http://www.ebi.ac.uk/pdbsum/6uro PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uro ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 6uro" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6uro" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[WD-repeat protein 3D structures\|WD-repeat protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 01:14, 7 March 2020

OCA — Sat, 07 Mar 2020 01:14:33 GMT

←Older revision		Revision as of 01:14, 7 March 2020
Line 1:		Line 1:

	==Cryo-EM structure of human CPSF160-WDR33-CPSF30-PAS RNA-CstF77 complex==		==Cryo-EM structure of human CPSF160-WDR33-CPSF30-PAS RNA-CstF77 complex==
-	<~~StructureSection~~ load='6uro' size='340' side='right'caption='[[6uro]], [[Resolution\|resolution]] 3.60Å' scene=''>	+	<SX load='6uro' size='340' side='right' viewer='molstar' caption='[[6uro]], [[Resolution\|resolution]] 3.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6uro]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6URO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6URO FirstGlance]. <br>		<table><tr><td colspan='2'>[[6uro]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6URO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6URO FirstGlance]. <br>
Line 23:		Line 23:
	<references/>		<references/>
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: Human]]		[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 13:26, 18 December 2019

OCA — Wed, 18 Dec 2019 13:26:58 GMT

←Older revision		Revision as of 13:26, 18 December 2019
Line 3:		Line 3:
	<StructureSection load='6uro' size='340' side='right'caption='[[6uro]], [[Resolution\|resolution]] 3.60Å' scene=''>		<StructureSection load='6uro' size='340' side='right'caption='[[6uro]], [[Resolution\|resolution]] 3.60Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6uro]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6URO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6URO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6uro]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6URO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6URO FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6urg\|6urg]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6urg\|6urg]]</td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CPSF1, CPSF160 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), WDR33, WDC146 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CPSF4, CPSF30, NAR, NEB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), CSTF3 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6uro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uro OCA], [http://pdbe.org/6uro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uro RCSB], [http://www.ebi.ac.uk/pdbsum/6uro PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uro ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6uro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uro OCA], [http://pdbe.org/6uro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uro RCSB], [http://www.ebi.ac.uk/pdbsum/6uro PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uro ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/CPSF1_HUMAN CPSF1_HUMAN]] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.<ref>PMID:14749727</ref> [[http://www.uniprot.org/uniprot/CPSF4_HUMAN CPSF4_HUMAN]] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U).<ref>PMID:9224719</ref> <ref>PMID:14749727</ref> [[http://www.uniprot.org/uniprot/WDR33_HUMAN WDR33_HUMAN]] Essential for both cleavage and polyadenylation of pre-mRNA 3' ends.<ref>PMID:19217410</ref> [[http://www.uniprot.org/uniprot/CSTF3_HUMAN CSTF3_HUMAN]] One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs.		[[http://www.uniprot.org/uniprot/CPSF1_HUMAN CPSF1_HUMAN]] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.<ref>PMID:14749727</ref> [[http://www.uniprot.org/uniprot/CPSF4_HUMAN CPSF4_HUMAN]] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U).<ref>PMID:9224719</ref> <ref>PMID:14749727</ref> [[http://www.uniprot.org/uniprot/WDR33_HUMAN WDR33_HUMAN]] Essential for both cleavage and polyadenylation of pre-mRNA 3' ends.<ref>PMID:19217410</ref> [[http://www.uniprot.org/uniprot/CSTF3_HUMAN CSTF3_HUMAN]] One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The mammalian pre-mRNA 3'-end-processing machinery consists of cleavage and polyadenylation specificity factor (CPSF), cleavage stimulation factor (CstF), and other proteins, but the overall architecture of this machinery remains unclear. CPSF contains two functionally distinct modules: a cleavage factor (mCF) and a polyadenylation specificity factor (mPSF). Here, we have produced recombinant human CPSF and CstF and examined these factors by electron microscopy (EM). We find that mPSF is the organizational core of the machinery, while the conformations of mCF and CstF and the position of mCF relative to mPSF are highly variable. We have identified by cryo-EM a segment in CPSF100 that tethers mCF to mPSF, and we have named it the PSF interaction motif (PIM). Mutations in the PIM can abolish CPSF formation, indicating that it is a crucial contact in CPSF. We have also obtained reconstructions of mCF and CstF77 by cryo-EM, assembled around the mPSF core.
		+
		+	Structural Insights into the Human Pre-mRNA 3'-End Processing Machinery.,Zhang Y, Sun Y, Shi Y, Walz T, Tong L Mol Cell. 2019 Nov 25. pii: S1097-2765(19)30834-2. doi:, 10.1016/j.molcel.2019.11.005. PMID:31810758<ref>PMID:31810758</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6uro" style="background-color:#fffaf0;"></div>
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Sun, Y]]		[[Category: Sun, Y]]

OCA at 07:03, 27 November 2019

OCA — Wed, 27 Nov 2019 07:03:14 GMT

←Older revision		Revision as of 07:03, 27 November 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6uro is ~~ON HOLD until Paper Publication~~	+	==Cryo-EM structure of human CPSF160-WDR33-CPSF30-PAS RNA-CstF77 complex==
-		+	<StructureSection load='6uro' size='340' side='right'caption='[[6uro]], [[Resolution\|resolution]] 3.60Å' scene=''>
-	~~Authors~~: ~~Sun~~, Y., ~~Zhang~~, Y., ~~Walz, T~~., ~~Tong, L~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6uro]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6URO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6URO FirstGlance]. <br>
-	~~Description~~: ~~Cryo-EM structure~~ of ~~human CPSF160~~-~~WDR33~~-~~CPSF30~~-~~PAS~~ RNA-~~CstF77 complex~~	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6urg\|6urg]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6uro FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uro OCA], [http://pdbe.org/6uro PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uro RCSB], [http://www.ebi.ac.uk/pdbsum/6uro PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uro ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CPSF1_HUMAN CPSF1_HUMAN]] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that plays a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. This subunit is involved in the RNA recognition step of the polyadenylation reaction.<ref>PMID:14749727</ref> [[http://www.uniprot.org/uniprot/CPSF4_HUMAN CPSF4_HUMAN]] Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U).<ref>PMID:9224719</ref> <ref>PMID:14749727</ref> [[http://www.uniprot.org/uniprot/WDR33_HUMAN WDR33_HUMAN]] Essential for both cleavage and polyadenylation of pre-mRNA 3' ends.<ref>PMID:19217410</ref> [[http://www.uniprot.org/uniprot/CSTF3_HUMAN CSTF3_HUMAN]] One of the multiple factors required for polyadenylation and 3'-end cleavage of mammalian pre-mRNAs.
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Sun, Y]]
	[[Category: Tong, L]]		[[Category: Tong, L]]
		+	[[Category: Walz, T]]
	[[Category: Zhang, Y]]		[[Category: Zhang, Y]]
-	[[Category: ~~Walz, T~~]]	+	[[Category: Aauaaa polyadenylation signal]]
-	[[Category: ~~Sun, Y~~]]	+	[[Category: Mpsf]]
		+	[[Category: Pre-mrna 3'-end processing]]
		+	[[Category: Rna binding]]
		+	[[Category: Rna binding protein-rna complex]]

OCA at 09:33, 13 November 2019

OCA — Wed, 13 Nov 2019 09:33:02 GMT

←Older revision		Revision as of 09:33, 13 November 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6uro is ON HOLD	+	The entry 6uro is ON HOLD until Paper Publication

	Authors: Sun, Y., Zhang, Y., Walz, T., Tong, L.		Authors: Sun, Y., Zhang, Y., Walz, T., Tong, L.
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	Description: Cryo-EM structure of human CPSF160-WDR33-CPSF30-PAS RNA-CstF77 complex		Description: Cryo-EM structure of human CPSF160-WDR33-CPSF30-PAS RNA-CstF77 complex
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Zhang, Y]]
	[[Category: Tong, L]]		[[Category: Tong, L]]
		+	[[Category: Zhang, Y]]
	[[Category: Walz, T]]		[[Category: Walz, T]]
	[[Category: Sun, Y]]		[[Category: Sun, Y]]

OCA: Protected "6uro" [edit=sysop:move=sysop]

OCA — Wed, 30 Oct 2019 07:43:45 GMT

Protected "6uro" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:43, 30 October 2019

OCA: New page: '''Unreleased structure''' The entry 6uro is ON HOLD Authors: Sun, Y., Zhang, Y., Walz, T., Tong, L. Description: Cryo-EM structure of human CPSF160-WDR33-CPSF30-PAS RNA-CstF77 complex...

OCA — Wed, 30 Oct 2019 07:43:44 GMT

New page: '''Unreleased structure''' The entry 6uro is ON HOLD Authors: Sun, Y., Zhang, Y., Walz, T., Tong, L. Description: Cryo-EM structure of human CPSF160-WDR33-CPSF30-PAS RNA-CstF77 complex...

New page

'''Unreleased structure'''

The entry 6uro is ON HOLD

Authors: Sun, Y., Zhang, Y., Walz, T., Tong, L.

Description: Cryo-EM structure of human CPSF160-WDR33-CPSF30-PAS RNA-CstF77 complex
[[Category: Unreleased Structures]]
[[Category: Zhang, Y]]
[[Category: Tong, L]]
[[Category: Walz, T]]
[[Category: Sun, Y]]