6uvo - Revision history

OCA at 07:59, 11 October 2023

OCA — Wed, 11 Oct 2023 07:59:16 GMT

←Older revision		Revision as of 07:59, 11 October 2023
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	<StructureSection load='6uvo' size='340' side='right'caption='[[6uvo]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='6uvo' size='340' side='right'caption='[[6uvo]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6uvo]] is a 3 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Hrsva Hrsva~~] and [~~http~~://en.wikipedia.org/wiki/Human ~~Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UVO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6UVO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6uvo]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Human_respiratory_syncytial_virus_A2 Human respiratory syncytial virus A2]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UVO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UVO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6uvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uvo OCA], [~~http~~://pdbe.org/6uvo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6uvo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6uvo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6uvo ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6uvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uvo OCA], [https://pdbe.org/6uvo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6uvo RCSB], [https://www.ebi.ac.uk/pdbsum/6uvo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6uvo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/GLYC_HRSVA GLYC_HRSVA]] Attaches the virion to the host cell membrane by interacting with heparan sulfate, initiating the infection. Interacts with host CX3CR1, the receptor for the CX3C chemokine fractalkine, to modulate the immune response and facilitate infection. Unlike the other paramyxovirus attachment proteins, lacks both neuraminidase and hemagglutinating activities. Secreted glycoprotein G helps RSV escape antibody-dependent restriction of replication by acting as an antigen decoy and by modulating the activity of leukocytes bearing Fcgamma receptors.	+	[https://www.uniprot.org/uniprot/GLYC_HRSVA GLYC_HRSVA] Attaches the virion to the host cell membrane by interacting with heparan sulfate, initiating the infection. Interacts with host CX3CR1, the receptor for the CX3C chemokine fractalkine, to modulate the immune response and facilitate infection. Unlike the other paramyxovirus attachment proteins, lacks both neuraminidase and hemagglutinating activities. Secreted glycoprotein G helps RSV escape antibody-dependent restriction of replication by acting as an antigen decoy and by modulating the activity of leukocytes bearing Fcgamma receptors.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 17:		Line 18:
	</div>		</div>
	<div class="pdbe-citations 6uvo" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6uvo" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Monoclonal Antibodies 3D structures\|Monoclonal Antibodies 3D structures]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Hrsva~~]]	+	[[Category: Homo sapiens]]
-	[[Category: Human]]	+	[[Category: Human respiratory syncytial virus A2]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Castrejon, A M.Nunez]]~~	+	[[Category: Dillen J]]
-	[[Category: Dillen, J]]	+	[[Category: DuBois RM]]
-	[[Category: DuBois~~, R M~~]]	+	[[Category: Fedechkin SO]]
-	[[Category: Fedechkin~~, S O~~]]	+	[[Category: George NL]]
-	[[Category: George~~, N L~~]]	+	[[Category: Kauvar LM]]
-	[[Category: Kauvar~~, L M~~]]	+	[[Category: Nunez Castrejon AM]]
-	[[Category: ~~Antibody]]~~	+
-	~~[[Category: G glycoprotein]]~~	+
-	~~[[Category: Glycoprotein]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Rsv]]~~	+
-	~~[[Category: Viral attachment protein]]~~	+
-	~~[[Category: Viral protein~~]]	+

OCA at 12:25, 1 January 2020

OCA — Wed, 01 Jan 2020 12:25:28 GMT

←Older revision		Revision as of 12:25, 1 January 2020
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	<StructureSection load='6uvo' size='340' side='right'caption='[[6uvo]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='6uvo' size='340' side='right'caption='[[6uvo]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6uvo]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UVO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UVO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6uvo]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/Hrsva Hrsva] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UVO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UVO FirstGlance]. <br>
	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6uvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uvo OCA], [http://pdbe.org/6uvo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uvo RCSB], [http://www.ebi.ac.uk/pdbsum/6uvo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uvo ProSAT]</span></td></tr>		</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6uvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uvo OCA], [http://pdbe.org/6uvo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uvo RCSB], [http://www.ebi.ac.uk/pdbsum/6uvo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uvo ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/GLYC_HRSVA GLYC_HRSVA]] Attaches the virion to the host cell membrane by interacting with heparan sulfate, initiating the infection. Interacts with host CX3CR1, the receptor for the CX3C chemokine fractalkine, to modulate the immune response and facilitate infection. Unlike the other paramyxovirus attachment proteins, lacks both neuraminidase and hemagglutinating activities. Secreted glycoprotein G helps RSV escape antibody-dependent restriction of replication by acting as an antigen decoy and by modulating the activity of leukocytes bearing Fcgamma receptors.		[[http://www.uniprot.org/uniprot/GLYC_HRSVA GLYC_HRSVA]] Attaches the virion to the host cell membrane by interacting with heparan sulfate, initiating the infection. Interacts with host CX3CR1, the receptor for the CX3C chemokine fractalkine, to modulate the immune response and facilitate infection. Unlike the other paramyxovirus attachment proteins, lacks both neuraminidase and hemagglutinating activities. Secreted glycoprotein G helps RSV escape antibody-dependent restriction of replication by acting as an antigen decoy and by modulating the activity of leukocytes bearing Fcgamma receptors.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Respiratory syncytial virus (RSV) is a top cause of severe lower respiratory tract disease and mortality in infants and the elderly. Currently, no vaccine or effective treatment exists for RSV. The RSV G glycoprotein mediates viral attachment to cells and contributes to pathogenesis by modulating host immunity through interactions with the human chemokine receptor CX3CR1. Antibodies targeting the RSV G central conserved domain are protective in both prophylactic and post-infection animal models. Here we describe the crystal structure of the broadly-neutralizing human monoclonal antibody 3G12 bound to the RSV G central conserved domain. Antibody 3G12 binds to a conformational epitope composed of highly conserved residues, explaining its broad neutralization activity. Surprisingly, RSV G complexed with 3G12 adopts a distinct conformation not observed in previously described RSV G-antibody structures. Comparison to other structures reveals that the RSV G central conserved domain is flexible and can adopt multiple conformations in the regions flanking the cysteine noose. We also show that restriction of RSV G flexibility with a proline mutation abolishes binding to antibody 3G12 but not antibody 3D3, which recognizes a different conformation of RSV G. Our studies provide new insights for rational vaccine design, indicating the importance of preserving both the global structural integrity of antigens and local conformational flexibility at antigenic sites, which may elicit a more diverse antibody response and broader protection against infection and disease.IMPORTANCE Respiratory syncytial virus (RSV) causes severe respiratory infections in infants, young children, and the elderly, and currently no licensed vaccine exists. In this study, we describe the crystal structure of the RSV surface glycoprotein G in complex with a broadly-neutralizing human monoclonal antibody. The antibody binds to RSV G at a highly conserved region stabilized by two disulfide bonds, but it captures RSV G in a conformation not previously observed, revealing that this region is both structured and flexible. Importantly, our findings provide insight for the design of vaccines that elicit diverse antibodies, which may provide broad protection from infection and disease.
		+
		+	Conformational flexibility in respiratory syncytial virus G neutralizing epitopes.,Fedechkin SO, George NL, Nunez Castrejon AM, Dillen JR, Kauvar LM, DuBois RM J Virol. 2019 Dec 18. pii: JVI.01879-19. doi: 10.1128/JVI.01879-19. PMID:31852779<ref>PMID:31852779</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6uvo" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Hrsva]]
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Castrejon, A M.Nunez]]		[[Category: Castrejon, A M.Nunez]]

OCA at 08:02, 18 December 2019

OCA — Wed, 18 Dec 2019 08:02:34 GMT

←Older revision		Revision as of 08:02, 18 December 2019
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6uvo is ~~ON HOLD~~ ~~until Nov 03 2021~~	+	==Structure of antibody 3G12 bound to the central conserved domain of RSV G==
-		+	<StructureSection load='6uvo' size='340' side='right'caption='[[6uvo]], [[Resolution\|resolution]] 2.90Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6uvo]] is a 3 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UVO OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UVO FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6uvo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uvo OCA], [http://pdbe.org/6uvo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uvo RCSB], [http://www.ebi.ac.uk/pdbsum/6uvo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uvo ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/GLYC_HRSVA GLYC_HRSVA]] Attaches the virion to the host cell membrane by interacting with heparan sulfate, initiating the infection. Interacts with host CX3CR1, the receptor for the CX3C chemokine fractalkine, to modulate the immune response and facilitate infection. Unlike the other paramyxovirus attachment proteins, lacks both neuraminidase and hemagglutinating activities. Secreted glycoprotein G helps RSV escape antibody-dependent restriction of replication by acting as an antigen decoy and by modulating the activity of leukocytes bearing Fcgamma receptors.
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Castrejon, A M.Nunez]]
		+	[[Category: Dillen, J]]
		+	[[Category: DuBois, R M]]
		+	[[Category: Fedechkin, S O]]
		+	[[Category: George, N L]]
		+	[[Category: Kauvar, L M]]
		+	[[Category: Antibody]]
		+	[[Category: G glycoprotein]]
		+	[[Category: Glycoprotein]]
		+	[[Category: Immune system]]
		+	[[Category: Rsv]]
		+	[[Category: Viral attachment protein]]
		+	[[Category: Viral protein]]

OCA at 13:43, 11 December 2019

OCA — Wed, 11 Dec 2019 13:43:03 GMT

←Older revision		Revision as of 13:43, 11 December 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6uvo is ON HOLD	+	The entry 6uvo is ON HOLD until Nov 03 2021

	Authors:		Authors:

OCA: Protected "6uvo" [edit=sysop:move=sysop]

OCA — Wed, 13 Nov 2019 09:36:27 GMT

Protected "6uvo" [edit=sysop:move=sysop]

←Older revision

Revision as of 09:36, 13 November 2019

OCA: New page: '''Unreleased structure''' The entry 6uvo is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 13 Nov 2019 09:36:26 GMT

New page: '''Unreleased structure''' The entry 6uvo is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6uvo is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]