6uzl - Revision history

OCA at 14:34, 6 March 2024

OCA — Wed, 06 Mar 2024 14:34:57 GMT

←Older revision		Revision as of 14:34, 6 March 2024
Line 3:		Line 3:
	<SX load='6uzl' size='340' side='right' viewer='molstar' caption='[[6uzl]], [[Resolution\|resolution]] 4.40Å' scene=''>		<SX load='6uzl' size='340' side='right' viewer='molstar' caption='[[6uzl]], [[Resolution\|resolution]] 4.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6uzl]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~"bacillus_coli"_migula_1895 "bacillus~~ coli~~" migula 1895~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UZL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6UZL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6uzl]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UZL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6UZL FirstGlance]. <br>
-	</td></tr><tr id='~~activity~~'><td class="sblockLbl"><b>~~Activity~~:</b></td><td class="sblockDat"~~><span class~~=~~'plainlinks'~~>[~~http://en.wikipedia.org/wiki/ABC-type_lipid_A-core_oligosaccharide_transporter ABC-type lipid A-core oligosaccharide transporter~~]~~, with EC number [http://www~~.~~brenda-enzymes.info/php/result_flat.php4?ecno=7.5.2.6 7.5.2.6] </span>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.4Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6uzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uzl OCA], [~~http~~://pdbe.org/6uzl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6uzl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6uzl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6uzl ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6uzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uzl OCA], [https://pdbe.org/6uzl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6uzl RCSB], [https://www.ebi.ac.uk/pdbsum/6uzl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6uzl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/~~C3TGA2_ECOLX C3TGA2_ECOLX]~~] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.~~[HAMAP-Rule:MF_01703][SAAS:SAAS00055332]~~	+	[https://www.uniprot.org/uniprot/MSBA_ECOLI MSBA_ECOLI] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Previously we introduced peptidiscs as an alternative to detergents to stabilize membrane proteins in solution (Carlson et al., 2018). Here, we present 'on-gradient' reconstitution, a new gentle approach for the reconstitution of labile membrane-protein complexes, and used it to reconstitute Rhodobacter sphaeroides reaction center complexes, demonstrating that peptidiscs can adapt to transmembrane domains of very different sizes and shapes. Using the conventional 'on-bead' approach, we reconstituted Escherichia coli proteins MsbA and MscS and find that peptidiscs stabilize them in their native conformation and allow for high-resolution structure determination by cryo-electron microscopy. The structures reveal that peptidisc peptides can arrange around transmembrane proteins differently, thus revealing the structural basis for why peptidiscs can stabilize such a large variety of membrane proteins. Together, our results establish the gentle and easy-to-use peptidiscs as a potentially universal alternative to detergents as a means to stabilize membrane proteins in solution for structural and functional studies.	+
-		+
-	New approach for membrane protein reconstitution into peptidiscs and basis for their adaptability to different proteins.,Angiulli G, Dhupar HS, Suzuki H, Wason IS, Duong Van Hoa F, Walz T Elife. 2020 Mar 3;9. pii: 53530. doi: 10.7554/eLife.53530. PMID:32125274<ref>PMID:32125274</ref>	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6uzl" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</SX>		</SX>
-	[[Category: ~~Bacillus~~ coli ~~migula 1895]]~~	+	[[Category: Escherichia coli]]
-	~~[[Category: ABC-type lipid A-core oligosaccharide transporter~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Angiulli, G]]	+	[[Category: Angiulli G]]
-	[[Category: Dhupar~~, H S~~]]	+	[[Category: Dhupar HS]]
-	[[Category: Hoa, F ~~Duong Van~~]]	+	[[Category: Duong Van Hoa F]]
-	[[Category: Suzuki, H]]	+	[[Category: Suzuki H]]
-	[[Category: Walz, T]]	+	[[Category: Walz T]]
-	[[Category: Wason~~, I S]]~~	+	[[Category: Wason IS]]
-	~~[[Category: Abc transporter]]~~	+
-	~~[[Category: Membrane mimetic]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Peptidisc]]~~	+
-	~~[[Category: Translocase~~]]	+

OCA at 06:57, 11 April 2020

OCA — Sat, 11 Apr 2020 06:57:32 GMT

←Older revision		Revision as of 06:57, 11 April 2020
Line 3:		Line 3:
	<SX load='6uzl' size='340' side='right' viewer='molstar' caption='[[6uzl]], [[Resolution\|resolution]] 4.40Å' scene=''>		<SX load='6uzl' size='340' side='right' viewer='molstar' caption='[[6uzl]], [[Resolution\|resolution]] 4.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6uzl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UZL OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6UZL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6uzl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UZL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6UZL FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/ABC-type_lipid_A-core_oligosaccharide_transporter ABC-type lipid A-core oligosaccharide transporter], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.5.2.6 7.5.2.6] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/ABC-type_lipid_A-core_oligosaccharide_transporter ABC-type lipid A-core oligosaccharide transporter], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.5.2.6 7.5.2.6] </span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6uzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uzl OCA], [http://pdbe.org/6uzl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uzl RCSB], [http://www.ebi.ac.uk/pdbsum/6uzl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uzl ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6uzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uzl OCA], [http://pdbe.org/6uzl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uzl RCSB], [http://www.ebi.ac.uk/pdbsum/6uzl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uzl ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==

OCA at 10:29, 18 March 2020

OCA — Wed, 18 Mar 2020 10:29:58 GMT

←Older revision		Revision as of 10:29, 18 March 2020
Line 3:		Line 3:
	<SX load='6uzl' size='340' side='right' viewer='molstar' caption='[[6uzl]], [[Resolution\|resolution]] 4.40Å' scene=''>		<SX load='6uzl' size='340' side='right' viewer='molstar' caption='[[6uzl]], [[Resolution\|resolution]] 4.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6uzl]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UZL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6uzl]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_coli"_migula_1895 "bacillus coli" migula 1895]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UZL FirstGlance]. <br>
	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/ABC-type_lipid_A-core_oligosaccharide_transporter ABC-type lipid A-core oligosaccharide transporter], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.5.2.6 7.5.2.6] </span></td></tr>		</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/ABC-type_lipid_A-core_oligosaccharide_transporter ABC-type lipid A-core oligosaccharide transporter], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.5.2.6 7.5.2.6] </span></td></tr>
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6uzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uzl OCA], [http://pdbe.org/6uzl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uzl RCSB], [http://www.ebi.ac.uk/pdbsum/6uzl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uzl ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6uzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uzl OCA], [http://pdbe.org/6uzl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uzl RCSB], [http://www.ebi.ac.uk/pdbsum/6uzl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uzl ProSAT]</span></td></tr>
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/C3TGA2_ECOLX C3TGA2_ECOLX]] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.[HAMAP-Rule:MF_01703][SAAS:SAAS00055332]		[[http://www.uniprot.org/uniprot/C3TGA2_ECOLX C3TGA2_ECOLX]] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.[HAMAP-Rule:MF_01703][SAAS:SAAS00055332]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Previously we introduced peptidiscs as an alternative to detergents to stabilize membrane proteins in solution (Carlson et al., 2018). Here, we present 'on-gradient' reconstitution, a new gentle approach for the reconstitution of labile membrane-protein complexes, and used it to reconstitute Rhodobacter sphaeroides reaction center complexes, demonstrating that peptidiscs can adapt to transmembrane domains of very different sizes and shapes. Using the conventional 'on-bead' approach, we reconstituted Escherichia coli proteins MsbA and MscS and find that peptidiscs stabilize them in their native conformation and allow for high-resolution structure determination by cryo-electron microscopy. The structures reveal that peptidisc peptides can arrange around transmembrane proteins differently, thus revealing the structural basis for why peptidiscs can stabilize such a large variety of membrane proteins. Together, our results establish the gentle and easy-to-use peptidiscs as a potentially universal alternative to detergents as a means to stabilize membrane proteins in solution for structural and functional studies.
		+
		+	New approach for membrane protein reconstitution into peptidiscs and basis for their adaptability to different proteins.,Angiulli G, Dhupar HS, Suzuki H, Wason IS, Duong Van Hoa F, Walz T Elife. 2020 Mar 3;9. pii: 53530. doi: 10.7554/eLife.53530. PMID:32125274<ref>PMID:32125274</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6uzl" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</SX>		</SX>
		+	[[Category: Bacillus coli migula 1895]]
	[[Category: ABC-type lipid A-core oligosaccharide transporter]]		[[Category: ABC-type lipid A-core oligosaccharide transporter]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 01:15, 7 March 2020

OCA — Sat, 07 Mar 2020 01:15:45 GMT

←Older revision		Revision as of 01:15, 7 March 2020
Line 1:		Line 1:

	==Cryo-EM structure of nucleotide-free MsbA reconstituted into peptidiscs, conformation 2==		==Cryo-EM structure of nucleotide-free MsbA reconstituted into peptidiscs, conformation 2==
-	<~~StructureSection~~ load='6uzl' size='340' side='right'caption='[[6uzl]], [[Resolution\|resolution]] 4.40Å' scene=''>	+	<SX load='6uzl' size='340' side='right' viewer='molstar' caption='[[6uzl]], [[Resolution\|resolution]] 4.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
	<table><tr><td colspan='2'>[[6uzl]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UZL FirstGlance]. <br>		<table><tr><td colspan='2'>[[6uzl]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UZL FirstGlance]. <br>
Line 10:		Line 10:
	[[http://www.uniprot.org/uniprot/C3TGA2_ECOLX C3TGA2_ECOLX]] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.[HAMAP-Rule:MF_01703][SAAS:SAAS00055332]		[[http://www.uniprot.org/uniprot/C3TGA2_ECOLX C3TGA2_ECOLX]] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.[HAMAP-Rule:MF_01703][SAAS:SAAS00055332]
	__TOC__		__TOC__
-	</~~StructureSection~~>	+	</SX>
	[[Category: ABC-type lipid A-core oligosaccharide transporter]]		[[Category: ABC-type lipid A-core oligosaccharide transporter]]
	[[Category: Large Structures]]		[[Category: Large Structures]]

OCA at 06:57, 4 March 2020

OCA — Wed, 04 Mar 2020 06:57:09 GMT

←Older revision		Revision as of 06:57, 4 March 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6uzl is ~~ON HOLD until Paper Publication~~	+	==Cryo-EM structure of nucleotide-free MsbA reconstituted into peptidiscs, conformation 2==
-		+	<StructureSection load='6uzl' size='340' side='right'caption='[[6uzl]], [[Resolution\|resolution]] 4.40Å' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>[[6uzl]] is a 2 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6UZL OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6UZL FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/ABC-type_lipid_A-core_oligosaccharide_transporter ABC-type lipid A-core oligosaccharide transporter], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=7.5.2.6 7.5.2.6] </span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6uzl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6uzl OCA], [http://pdbe.org/6uzl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6uzl RCSB], [http://www.ebi.ac.uk/pdbsum/6uzl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6uzl ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/C3TGA2_ECOLX C3TGA2_ECOLX]] Involved in lipid A export and possibly also in glycerophospholipid export and for biogenesis of the outer membrane. Transmembrane domains (TMD) form a pore in the inner membrane and the ATP-binding domain (NBD) is responsible for energy generation.[HAMAP-Rule:MF_01703][SAAS:SAAS00055332]
		+	__TOC__
		+	</StructureSection>
		+	[[Category: ABC-type lipid A-core oligosaccharide transporter]]
		+	[[Category: Large Structures]]
		+	[[Category: Angiulli, G]]
		+	[[Category: Dhupar, H S]]
		+	[[Category: Hoa, F Duong Van]]
		+	[[Category: Suzuki, H]]
		+	[[Category: Walz, T]]
		+	[[Category: Wason, I S]]
		+	[[Category: Abc transporter]]
		+	[[Category: Membrane mimetic]]
		+	[[Category: Membrane protein]]
		+	[[Category: Peptidisc]]
		+	[[Category: Translocase]]

OCA at 13:43, 11 December 2019

OCA — Wed, 11 Dec 2019 13:43:21 GMT

←Older revision		Revision as of 13:43, 11 December 2019
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6uzl is ON HOLD	+	The entry 6uzl is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6uzl" [edit=sysop:move=sysop]

OCA — Wed, 27 Nov 2019 05:23:14 GMT

Protected "6uzl" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:23, 27 November 2019

OCA: New page: '''Unreleased structure''' The entry 6uzl is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 27 Nov 2019 05:23:12 GMT

New page: '''Unreleased structure''' The entry 6uzl is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6uzl is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]