6v02 - Revision history

OCA at 08:01, 11 October 2023

OCA — Wed, 11 Oct 2023 08:01:31 GMT

←Older revision		Revision as of 08:01, 11 October 2023
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	==N-terminal 5 domains of CI-MPR==		==N-terminal 5 domains of CI-MPR==
-	<StructureSection load='6v02' size='340' side='right'caption='[[6v02]]' scene=''>	+	<StructureSection load='6v02' size='340' side='right'caption='[[6v02]], [[Resolution\|resolution]] 2.46Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V02 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6V02 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6v02]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V02 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6V02 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6v02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v02 OCA], [~~http~~://pdbe.org/6v02 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6v02 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6v02 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6v02 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.46Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6v02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v02 OCA], [https://pdbe.org/6v02 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6v02 RCSB], [https://www.ebi.ac.uk/pdbsum/6v02 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6v02 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MPRI_HUMAN MPRI_HUMAN] Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.<ref>PMID:10900005</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The cation-independent mannose 6-phosphate receptor (CI-MPR, IGF2 receptor or CD222), is a multifunctional glycoprotein required for normal development. Through the receptor's ability to bind unrelated extracellular and intracellular ligands, it participates in numerous functions including protein trafficking, lysosomal biogenesis, and regulation of cell growth. Clinically, endogenous CI-MPR delivers infused recombinant enzymes to lysosomes in the treatment of lysosomal storage diseases. Although four of the 15 domains comprising CI-MPR's extracellular region bind phosphorylated glycans on lysosomal enzymes, knowledge of how CI-MPR interacts with ~60 different lysosomal enzymes is limited. Here, we show by electron microscopy and hydroxyl radical protein footprinting that the N-terminal region of CI-MPR undergoes dynamic conformational changes as a consequence of ligand binding and different pH conditions. These data, coupled with X-ray crystallography, surface plasmon resonance and molecular modeling, allow us to propose a model explaining how high-affinity carbohydrate binding is achieved through allosteric domain cooperativity.
		+
		+	Allosteric regulation of lysosomal enzyme recognition by the cation-independent mannose 6-phosphate receptor.,Olson LJ, Misra SK, Ishihara M, Battaile KP, Grant OC, Sood A, Woods RJ, Kim JP, Tiemeyer M, Ren G, Sharp JS, Dahms NM Commun Biol. 2020 Sep 9;3(1):498. doi: 10.1038/s42003-020-01211-w. PMID:32908216<ref>PMID:32908216</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6v02" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Dahms NM]]		[[Category: Dahms NM]]
	[[Category: Kim J-JP]]		[[Category: Kim J-JP]]
	[[Category: Olson LJ]]		[[Category: Olson LJ]]

OCA at 07:09, 30 September 2020

OCA — Wed, 30 Sep 2020 07:09:00 GMT

←Older revision		Revision as of 07:09, 30 September 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6v02 ~~is ON HOLD until Paper Publication~~	+	==N-terminal 5 domains of CI-MPR==
-		+	<StructureSection load='6v02' size='340' side='right'caption='[[6v02]]' scene=''>
-	~~Authors~~: ~~Olson~~, L.J., ~~Dahms~~, N.M., ~~Kim~~, J.-~~J.P~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V02 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6V02 FirstGlance]. <br>
-	~~Description: N-terminal 5 domains of CI-MPR~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6v02 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v02 OCA], [http://pdbe.org/6v02 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v02 RCSB], [http://www.ebi.ac.uk/pdbsum/6v02 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v02 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Olson, L.J~~]]	+	__TOC__
-	[[Category: Kim, J.-~~J.P~~]]	+	</StructureSection>
-	[[Category: ~~Dahms, N.M~~]]	+	[[Category: Large Structures]]
		+	[[Category: Dahms NM]]
		+	[[Category: Kim J-JP]]
		+	[[Category: Olson LJ]]

OCA at 09:18, 29 July 2020

OCA — Wed, 29 Jul 2020 09:18:56 GMT

←Older revision		Revision as of 09:18, 29 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6v02 is ON HOLD	+	The entry 6v02 is ON HOLD until Paper Publication

	Authors: Olson, L.J., Dahms, N.M., Kim, J.-J.P.		Authors: Olson, L.J., Dahms, N.M., Kim, J.-J.P.
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	Description: N-terminal 5 domains of CI-MPR		Description: N-terminal 5 domains of CI-MPR
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Kim, J.-J.P]]
	[[Category: Olson, L.J]]		[[Category: Olson, L.J]]
		+	[[Category: Kim, J.-J.P]]
	[[Category: Dahms, N.M]]		[[Category: Dahms, N.M]]

OCA: Protected "6v02" [edit=sysop:move=sysop]

OCA — Wed, 27 Nov 2019 05:24:24 GMT

Protected "6v02" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:24, 27 November 2019

OCA: New page: '''Unreleased structure''' The entry 6v02 is ON HOLD Authors: Olson, L.J., Dahms, N.M., Kim, J.-J.P. Description: N-terminal 5 domains of CI-MPR Category: Unreleased Structures [[C...

OCA — Wed, 27 Nov 2019 05:24:22 GMT

New page: '''Unreleased structure''' The entry 6v02 is ON HOLD Authors: Olson, L.J., Dahms, N.M., Kim, J.-J.P. Description: N-terminal 5 domains of CI-MPR Category: Unreleased Structures [[C...

New page

'''Unreleased structure'''

The entry 6v02 is ON HOLD

Authors: Olson, L.J., Dahms, N.M., Kim, J.-J.P.

Description: N-terminal 5 domains of CI-MPR
[[Category: Unreleased Structures]]
[[Category: Kim, J.-J.P]]
[[Category: Olson, L.J]]
[[Category: Dahms, N.M]]