OCA at 08:10, 11 November 2020

OCA — Wed, 11 Nov 2020 08:10:48 GMT

←Older revision		Revision as of 08:10, 11 November 2020
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	==Ankyrin repeat and SOCS-box protein 9 (ASB9), ElonginB (ELOB), and ElonginC (ELOC) bound to its substrate Brain-type Creatine Kinase (CKB)==		==Ankyrin repeat and SOCS-box protein 9 (ASB9), ElonginB (ELOB), and ElonginC (ELOC) bound to its substrate Brain-type Creatine Kinase (CKB)==
-	<StructureSection load='6v9h' size='340' side='right'caption='[[6v9h]]' scene=''>	+	<StructureSection load='6v9h' size='340' side='right'caption='[[6v9h]], [[Resolution\|resolution]] 4.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V9H OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6V9H FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6v9h]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V9H OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6V9H FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6v9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v9h OCA], [http://pdbe.org/6v9h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v9h RCSB], [http://www.ebi.ac.uk/pdbsum/6v9h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v9h ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CKB, CKBB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), ASB9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), ELOC, TCEB1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), ELOB, TCEB2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Creatine_kinase Creatine kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.3.2 2.7.3.2] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6v9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v9h OCA], [http://pdbe.org/6v9h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v9h RCSB], [http://www.ebi.ac.uk/pdbsum/6v9h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v9h ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ELOB_HUMAN ELOB_HUMAN]] SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).<ref>PMID:7638163</ref> <ref>PMID:15590694</ref> The elongin BC complex seems to be involved as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes, including the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes.<ref>PMID:7638163</ref> <ref>PMID:15590694</ref> [[http://www.uniprot.org/uniprot/KCRB_HUMAN KCRB_HUMAN]] Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. [[http://www.uniprot.org/uniprot/ELOC_HUMAN ELOC_HUMAN]] SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).<ref>PMID:15590694</ref> The elongin BC complex seems to be involved as an adapter protein in the proteasomal degradation of target proteins via different E3 ubiquitin ligase complexes, including the von Hippel-Lindau ubiquitination complex CBC(VHL). By binding to BC-box motifs it seems to link target recruitment subunits, like VHL and members of the SOCS box family, to Cullin/RBX1 modules that activate E2 ubiquitination enzymes.<ref>PMID:15590694</ref> [[http://www.uniprot.org/uniprot/ASB9_HUMAN ASB9_HUMAN]] Substrate-recognition component of a SCF-like ECS (Elongin-Cullin-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes at least two forms of creatine kinase, CKB and CKMT1A.<ref>PMID:20302626</ref> <ref>PMID:22418839</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The Cullin 5 (CUL5) Ring E3 ligase uses adaptors Elongins B and C (ELOB/C) to bind different SOCS-box-containing substrate receptors, determining the substrate specificity of the ligase. The 18-member ankyrin and SOCS box (ASB) family is the largest substrate receptor family. Here we report cryo-EM data for the substrate, creatine kinase (CKB) bound to ASB9-ELOB/C, and for full-length CUL5 bound to the RING protein, RBX2, which binds various E2s. To date, no full structures are available either for a substrate-bound ASB nor for CUL5. Hydrogen-deuterium exchange (HDX-MS) mapped onto a full structural model of the ligase revealed long-range allostery extending from the substrate through CUL5. We propose a revised allosteric mechanism for how CUL-E3 ligases function. ASB9 and CUL5 behave as rigid rods, connected through a hinge provided by ELOB/C transmitting long-range allosteric crosstalk from the substrate through CUL5 to the RBX2 flexible linker.
		+
		+	Structure and dynamics of the ASB9 CUL-RING E3 Ligase.,Lumpkin RJ, Baker RW, Leschziner AE, Komives EA Nat Commun. 2020 Jun 8;11(1):2866. doi: 10.1038/s41467-020-16499-9. PMID:32513959<ref>PMID:32513959</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6v9h" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Creatine kinase 3D structures\|Creatine kinase 3D structures]]
		+	*[[Elongation factor 3D structures\|Elongation factor 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Creatine kinase]]
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Baker RW]]	+	[[Category: Baker, R W]]
-	[[Category: Komives EA]]	+	[[Category: Komives, E A]]
-	[[Category: Leschziner AE]]	+	[[Category: Leschziner, A E]]
-	[[Category: Lumpkin RJ]]	+	[[Category: Lumpkin, R J]]
		+	[[Category: Ankyrin repeat]]
		+	[[Category: Elongation factor]]
		+	[[Category: Transcription]]
		+	[[Category: Ubiquitin]]

OCA at 06:47, 29 April 2020

OCA — Wed, 29 Apr 2020 06:47:01 GMT

←Older revision		Revision as of 06:47, 29 April 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6v9h is ~~ON HOLD until Paper Publication~~	+	==Ankyrin repeat and SOCS-box protein 9 (ASB9), ElonginB (ELOB), and ElonginC (ELOC) bound to its substrate Brain-type Creatine Kinase (CKB)==
-		+	<StructureSection load='6v9h' size='340' side='right'caption='[[6v9h]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6V9H OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6V9H FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6v9h FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6v9h OCA], [http://pdbe.org/6v9h PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6v9h RCSB], [http://www.ebi.ac.uk/pdbsum/6v9h PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6v9h ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Baker RW]]
		+	[[Category: Komives EA]]
		+	[[Category: Leschziner AE]]
		+	[[Category: Lumpkin RJ]]

OCA at 14:14, 22 January 2020

OCA — Wed, 22 Jan 2020 14:14:51 GMT

←Older revision		Revision as of 14:14, 22 January 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6v9h is ON HOLD	+	The entry 6v9h is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6v9h" [edit=sysop:move=sysop]

OCA — Wed, 25 Dec 2019 07:32:26 GMT

Protected "6v9h" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:32, 25 December 2019

OCA: New page: '''Unreleased structure''' The entry 6v9h is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 25 Dec 2019 07:32:24 GMT

New page: '''Unreleased structure''' The entry 6v9h is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6v9h is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6v9h - Revision history

OCA at 08:10, 11 November 2020

OCA at 06:47, 29 April 2020

OCA at 14:14, 22 January 2020

OCA: Protected "6v9h" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6v9h is ON HOLD Authors: Description: Category: Unreleased Structures