6vhf - Revision history

OCA at 14:37, 6 March 2024

OCA — Wed, 06 Mar 2024 14:37:45 GMT

←Older revision		Revision as of 14:37, 6 March 2024
Line 3:		Line 3:
	<StructureSection load='6vhf' size='340' side='right'caption='[[6vhf]], [[Resolution\|resolution]] 2.31Å' scene=''>		<StructureSection load='6vhf' size='340' side='right'caption='[[6vhf]], [[Resolution\|resolution]] 2.31Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6vhf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VHF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6VHF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6vhf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VHF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VHF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.311Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6vhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vhf OCA], [~~http~~://pdbe.org/6vhf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6vhf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6vhf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6vhf ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vhf OCA], [https://pdbe.org/6vhf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vhf RCSB], [https://www.ebi.ac.uk/pdbsum/6vhf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vhf ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/G0S1N3_CHATD G0S1N3_CHATD]
-	COMPlex ASsociating with SET1 (COMPASS) is a histone H3 Lys-4 methyltransferase that typically marks the promoter region of actively transcribed genes. COMPASS is a multi-subunit complex in which the catalytic unit, SET1, is required for H3K4 methylation. An important subunit known to regulate SET1 methyltransferase activity is the CxxC zinc finger protein 1 (Cfp1). Cfp1 binds to COMPASS and is critical to maintain high level of H3K4me3 in cells but the mechanisms underlying its stimulatory activity is poorly understood. In this study, we show that Cfp1 only modestly activates COMPASS methyltransferase activity in vitro. Binding of Cfp1 to COMPASS is in part mediated by a new type of monovalent zinc finger (ZnF). This ZnF interacts with the COMPASS's subunits RbBP5 and disruption of this interaction blunts its methyltransferase activity in cells and in vivo. Collectively, our studies reveal that a novel form of ZnF on Cfp1 enables its integration into COMPASS and contributes to epigenetic signaling.	+
-		+
-	A non-canonical monovalent zinc finger stabilizes the integration of Cfp1 into the H3K4 methyltransferase complex COMPASS.,Yang Y, Joshi M, Takahashi YH, Ning Z, Qu Q, Brunzelle JS, Skiniotis G, Figeys D, Shilatifard A, Couture JF Nucleic Acids Res. 2020 Jan 10;48(1):~~421-431. doi: 10.1093~~/~~nar~~/~~gkz1037~~. ~~PMID:31724694<ref>PMID:31724694</ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.~~S. National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6vhf" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Couture~~, J F~~]]	+	[[Category: Couture JF]]
-	[[Category: Joshi, M]]	+	[[Category: Joshi M]]
-	~~[[Category: Cfp1]]~~	+
-	~~[[Category: Compass]]~~	+
-	~~[[Category: Epigenetic]]~~	+
-	~~[[Category: Histone]]~~	+
-	~~[[Category: Mll]]~~	+
-	~~[[Category: Peptide binding protein]]~~	+
-	~~[[Category: Rbbp5~~]]	+

OCA: Protected "6vhf" [edit=sysop:move=sysop]

OCA — Wed, 22 Jan 2020 16:23:45 GMT

Protected "6vhf" [edit=sysop:move=sysop]

←Older revision

Revision as of 16:23, 22 January 2020

OCA: New page: ==Crystal structure of RbBP5 interacting domain of Cfp1== == Struc...

OCA — Wed, 22 Jan 2020 16:23:43 GMT

New page: ==Crystal structure of RbBP5 interacting domain of Cfp1== <StructureSection load='6vhf' size='340' side='right'caption='6vhf, resolution 2.31Å' scene=''> == Struc...

New page

==Crystal structure of RbBP5 interacting domain of Cfp1==
<StructureSection load='6vhf' size='340' side='right'caption='[[6vhf]], [[Resolution|resolution]] 2.31Å' scene=''>
== Structural highlights ==
<table><tr><td colspan='2'>[[6vhf]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VHF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6VHF FirstGlance]. <br>
</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6vhf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vhf OCA], [http://pdbe.org/6vhf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vhf RCSB], [http://www.ebi.ac.uk/pdbsum/6vhf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vhf ProSAT]</span></td></tr>
</table>
<div style="background-color:#fffaf0;">
== Publication Abstract from PubMed ==
COMPlex ASsociating with SET1 (COMPASS) is a histone H3 Lys-4 methyltransferase that typically marks the promoter region of actively transcribed genes. COMPASS is a multi-subunit complex in which the catalytic unit, SET1, is required for H3K4 methylation. An important subunit known to regulate SET1 methyltransferase activity is the CxxC zinc finger protein 1 (Cfp1). Cfp1 binds to COMPASS and is critical to maintain high level of H3K4me3 in cells but the mechanisms underlying its stimulatory activity is poorly understood. In this study, we show that Cfp1 only modestly activates COMPASS methyltransferase activity in vitro. Binding of Cfp1 to COMPASS is in part mediated by a new type of monovalent zinc finger (ZnF). This ZnF interacts with the COMPASS's subunits RbBP5 and disruption of this interaction blunts its methyltransferase activity in cells and in vivo. Collectively, our studies reveal that a novel form of ZnF on Cfp1 enables its integration into COMPASS and contributes to epigenetic signaling.

A non-canonical monovalent zinc finger stabilizes the integration of Cfp1 into the H3K4 methyltransferase complex COMPASS.,Yang Y, Joshi M, Takahashi YH, Ning Z, Qu Q, Brunzelle JS, Skiniotis G, Figeys D, Shilatifard A, Couture JF Nucleic Acids Res. 2020 Jan 10;48(1):421-431. doi: 10.1093/nar/gkz1037. PMID:31724694<ref>PMID:31724694</ref>

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
</div>
<div class="pdbe-citations 6vhf" style="background-color:#fffaf0;"></div>
== References ==
<references/>
__TOC__
</StructureSection>
[[Category: Large Structures]]
[[Category: Couture, J F]]
[[Category: Joshi, M]]
[[Category: Cfp1]]
[[Category: Compass]]
[[Category: Epigenetic]]
[[Category: Histone]]
[[Category: Mll]]
[[Category: Peptide binding protein]]
[[Category: Rbbp5]]