6vjf - Revision history

OCA at 08:13, 11 October 2023

OCA — Wed, 11 Oct 2023 08:13:47 GMT

←Older revision		Revision as of 08:13, 11 October 2023
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	<StructureSection load='6vjf' size='340' side='right'caption='[[6vjf]], [[Resolution\|resolution]] 2.47Å' scene=''>		<StructureSection load='6vjf' size='340' side='right'caption='[[6vjf]], [[Resolution\|resolution]] 2.47Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6vjf]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Chatd Chatd~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VJF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6VJF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6vjf]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Chaetomium_thermophilum_var._thermophilum_DSM_1495 Chaetomium thermophilum var. thermophilum DSM 1495]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VJF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VJF FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>~~, ~~<scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.472Å</td></tr>
-	<tr id='~~related~~'><td class="sblockLbl"><b>[[~~Related_structure~~\|~~Related~~:]]</b></td><td class="sblockDat">~~[[6def\|6def]]~~<~~/td~~></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
-	<~~tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">CTHT_0061810 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])~~</td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vjf OCA], [https://pdbe.org/6vjf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vjf RCSB], [https://www.ebi.ac.uk/pdbsum/6vjf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vjf ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6vjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vjf OCA], [~~http~~://pdbe.org/6vjf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6vjf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6vjf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6vjf ProSAT]</span></td></tr>	+
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/G0SFF0_CHATD G0SFF0_CHATD]
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Chatd~~]]	+	[[Category: Chaetomium thermophilum var. thermophilum DSM 1495]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Chappie~~, J S~~]]	+	[[Category: Chappie JS]]
-	[[Category: Ford~~, M G.J~~]]	+	[[Category: Ford MGJ]]
-	[[Category: Tornabene~~, B A~~]]	+	[[Category: Tornabene BA]]
-	[[Category: Varlakhanova~~, N V]]~~	+	[[Category: Varlakhanova NV]]
-	~~[[Category: Bse]]~~	+
-	~~[[Category: Dynamin]]~~	+
-	~~[[Category: Dynamin superfamily]]~~	+
-	~~[[Category: Gcp]]~~	+
-	~~[[Category: Ged]]~~	+
-	~~[[Category: Gtpase]]~~	+
-	~~[[Category: Hydrolase]]~~	+
-	~~[[Category: P-loop~~]]	+

OCA at 06:47, 10 June 2020

OCA — Wed, 10 Jun 2020 06:47:12 GMT

←Older revision		Revision as of 06:47, 10 June 2020
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	<StructureSection load='6vjf' size='340' side='right'caption='[[6vjf]], [[Resolution\|resolution]] 2.47Å' scene=''>		<StructureSection load='6vjf' size='340' side='right'caption='[[6vjf]], [[Resolution\|resolution]] 2.47Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6vjf]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VJF OCA]. For a <b>guided tour on the structure components</b> use [http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6VJF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6vjf]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Chatd Chatd]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VJF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6VJF FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6def\|6def]]</td></tr>		<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6def\|6def]]</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://~~oca~~.~~weizmann.ac.il/oca-docs~~/fgij/fg.htm?mol=6vjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vjf OCA], [http://pdbe.org/6vjf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vjf RCSB], [http://www.ebi.ac.uk/pdbsum/6vjf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vjf ProSAT]</span></td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CTHT_0061810 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=759272 CHATD])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6vjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vjf OCA], [http://pdbe.org/6vjf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vjf RCSB], [http://www.ebi.ac.uk/pdbsum/6vjf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vjf ProSAT]</span></td></tr>
	</table>		</table>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
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	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Chatd]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Chappie, J S]]		[[Category: Chappie, J S]]

OCA at 06:45, 19 February 2020

OCA — Wed, 19 Feb 2020 06:45:05 GMT

←Older revision		Revision as of 06:45, 19 February 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	The ~~entry~~ 6vjf is ~~ON HOLD until Paper~~ Publication	+	==The P-Loop K to A mutation of C. therm Vps1 GTPase-BSE==
		+	<StructureSection load='6vjf' size='340' side='right'caption='[[6vjf]], [[Resolution\|resolution]] 2.47Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6vjf]] is a 4 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VJF OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6VJF FirstGlance]. <br>
		+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GCP:PHOSPHOMETHYLPHOSPHONIC+ACID+GUANYLATE+ESTER'>GCP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat">[[6def\|6def]]</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6vjf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vjf OCA], [http://pdbe.org/6vjf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vjf RCSB], [http://www.ebi.ac.uk/pdbsum/6vjf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vjf ProSAT]</span></td></tr>
		+	</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Dynamin-superfamily proteins (DSPs) are large self-assembling mechanochemical GTPases that harness GTP hydrolysis to drive membrane remodeling events needed for many cellular processes. Mutation to alanine of a fully conserved lysine within the P-loop of the DSP GTPase domain results in abrogation of GTPase activity. This mutant has been widely used in the context of several DSPs as a dominant-negative to impair DSP-dependent processes. However, the precise deficit of the P-loop K to A mutation remains an open question. Here, we use biophysical, biochemical and structural approaches to characterize this mutant in the context of the endosomal DSP Vps1. We show that the Vps1 P-loop K to A mutant binds nucleotide with an affinity similar to wild type but exhibits defects in the organization of the GTPase active site that explain the lack of hydrolysis. In cells, Vps1 and Dnm1 bearing the P-loop K to A mutation are defective in disassembly. These mutants become trapped in assemblies at the typical site of action of the DSP. This work provides mechanistic insight into the widely-used DSP P-loop K to A mutation and the basis of its dominant-negative effects in the cell.

-	~~Authors~~:	+	Structural and functional characterization of the dominant negative P-loop lysine mutation in the dynamin superfamily protein Vps1.,Tornabene BA, Varlakhanova NV, Hosford CJ, Chappie JS, Ford MGJ Protein Sci. 2020 Jan 24. doi: 10.1002/pro.3830. PMID:31981262<ref>PMID:31981262</ref>

-	~~Description~~:	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased~~ Structures]]	+	</div>
		+	<div class="pdbe-citations 6vjf" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Chappie, J S]]
		+	[[Category: Ford, M G.J]]
		+	[[Category: Tornabene, B A]]
		+	[[Category: Varlakhanova, N V]]
		+	[[Category: Bse]]
		+	[[Category: Dynamin]]
		+	[[Category: Dynamin superfamily]]
		+	[[Category: Gcp]]
		+	[[Category: Ged]]
		+	[[Category: Gtpase]]
		+	[[Category: Hydrolase]]
		+	[[Category: P-loop]]

OCA at 02:40, 13 February 2020

OCA — Thu, 13 Feb 2020 02:40:15 GMT

←Older revision		Revision as of 02:40, 13 February 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6vjf is ON HOLD	+	The entry 6vjf is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6vjf" [edit=sysop:move=sysop]

OCA — Wed, 22 Jan 2020 14:35:03 GMT

Protected "6vjf" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:35, 22 January 2020

OCA: New page: '''Unreleased structure''' The entry 6vjf is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 22 Jan 2020 14:35:01 GMT

New page: '''Unreleased structure''' The entry 6vjf is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6vjf is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]