OCA at 08:13, 11 October 2023

OCA — Wed, 11 Oct 2023 08:13:53 GMT

←Older revision		Revision as of 08:13, 11 October 2023
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	==Streptavidin mutant M112 (G26C/A46C)==		==Streptavidin mutant M112 (G26C/A46C)==
-	<StructureSection load='6vjl' size='340' side='right'caption='[[6vjl]]' scene=''>	+	<StructureSection load='6vjl' size='340' side='right'caption='[[6vjl]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VJL OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6VJL FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6vjl]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VJL OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VJL FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6vjl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vjl OCA], [~~http~~://pdbe.org/6vjl PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6vjl RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6vjl PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6vjl ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BTN:BIOTIN'>BTN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vjl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vjl OCA], [https://pdbe.org/6vjl PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vjl RCSB], [https://www.ebi.ac.uk/pdbsum/6vjl PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vjl ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Although high affinity binding between streptavidin and biotin is widely exploited, the accompanying low rate of dissociation prevents its use in many applications where rapid ligand release is also required. To combine extremely tight and reversible binding, we have introduced disulfide bonds into opposite sides of a flexible loop critical for biotin binding, creating streptavidin muteins (M88 and M112) with novel disulfide-switchable binding properties. Crystal structures reveal how each disulfide exerts opposing effects on structure and function. Whereas the disulfide in M112 disrupts the closed conformation to increase koff, the disulfide in M88 stabilizes the closed conformation, decreasing koff 260-fold relative to streptavidin. The simple and efficient reduction of this disulfide increases koff 19,000-fold, thus creating a reversible redox-dependent switch with 70-fold faster dissociation kinetics than streptavidin. The facile control of disulfide formation in M88 will enable the development of many new applications requiring high affinity and reversible binding.
		+
		+	Engineering a disulfide-gated switch in streptavidin enables reversible binding without sacrificing binding affinity.,Marangoni JM, Wu SC, Fogen D, Wong SL, Ng KKS Sci Rep. 2020 Jul 27;10(1):12483. doi: 10.1038/s41598-020-69357-5. PMID:32719366<ref>PMID:32719366</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6vjl" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Avidin 3D structures\|Avidin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Streptomyces avidinii]]
	[[Category: Fogen D]]		[[Category: Fogen D]]
	[[Category: Marangoni JM]]		[[Category: Marangoni JM]]

OCA at 10:29, 24 December 2020

OCA — Thu, 24 Dec 2020 10:29:55 GMT

←Older revision		Revision as of 10:29, 24 December 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6vjl is ~~ON HOLD until Paper Publication~~	+	==Streptavidin mutant M112 (G26C/A46C)==
-		+	<StructureSection load='6vjl' size='340' side='right'caption='[[6vjl]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VJL OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6VJL FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6vjl FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vjl OCA], [http://pdbe.org/6vjl PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vjl RCSB], [http://www.ebi.ac.uk/pdbsum/6vjl PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vjl ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Fogen D]]
		+	[[Category: Marangoni JM]]
		+	[[Category: Ng KKS]]
		+	[[Category: Wong SL]]
		+	[[Category: Wu SC]]

OCA at 13:34, 29 January 2020

OCA — Wed, 29 Jan 2020 13:34:13 GMT

←Older revision		Revision as of 13:34, 29 January 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6vjl is ON HOLD	+	The entry 6vjl is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6vjl" [edit=sysop:move=sysop]

OCA — Wed, 22 Jan 2020 14:35:59 GMT

Protected "6vjl" [edit=sysop:move=sysop]

←Older revision

Revision as of 14:35, 22 January 2020

OCA: New page: '''Unreleased structure''' The entry 6vjl is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 22 Jan 2020 14:35:57 GMT

New page: '''Unreleased structure''' The entry 6vjl is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6vjl is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6vjl - Revision history

OCA at 08:13, 11 October 2023

OCA at 10:29, 24 December 2020

OCA at 13:34, 29 January 2020

OCA: Protected "6vjl" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6vjl is ON HOLD Authors: Description: Category: Unreleased Structures