6voz - Revision history

OCA at 08:16, 11 October 2023

OCA — Wed, 11 Oct 2023 08:16:18 GMT

←Older revision		Revision as of 08:16, 11 October 2023
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	==Artificial Metalloproteins with Dinuclear Iron Centers==		==Artificial Metalloproteins with Dinuclear Iron Centers==
-	<StructureSection load='6voz' size='340' side='right'caption='[[6voz]]' scene=''>	+	<StructureSection load='6voz' size='340' side='right'caption='[[6voz]], [[Resolution\|resolution]] 1.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VOZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VOZ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6voz]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Streptomyces_avidinii Streptomyces avidinii]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VOZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VOZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6voz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6voz OCA], [https://pdbe.org/6voz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6voz RCSB], [https://www.ebi.ac.uk/pdbsum/6voz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6voz ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.3Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=KM3:{N-(4-{bis[(pyridin-2-yl-kappaN)methyl]amino-kappaN}butyl)-5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanamide}iron(3+)'>KM3</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6voz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6voz OCA], [https://pdbe.org/6voz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6voz RCSB], [https://www.ebi.ac.uk/pdbsum/6voz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6voz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SAV_STRAV SAV_STRAV] The biological function of streptavidin is not known. Forms a strong non-covalent specific complex with biotin (one molecule of biotin per subunit of streptavidin).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Dinuclear iron centers with a bridging hydroxido or oxido ligand form active sites within a variety of metalloproteins. A key feature of these sites is the ability of the protein to control the structures around the Fe centers, which leads to entatic states that are essential for function. To simulate this controlled environment, artificial proteins have been engineered using biotin-streptavidin (Sav) technology in which Fe complexes from adjacent subunits can assemble to form [Fe(III)-(mu-OH)-Fe(III)] cores. The assembly process is promoted by the site-specific localization of the Fe complexes within a subunit through the designed mutation of a tyrosinate side chain to coordinate the Fe centers. An important outcome is that the Sav host can regulate the Fe...Fe separation, which is known to be important for function in natural metalloproteins. Spectroscopic and structural studies from X-ray diffraction methods revealed uncommonly long Fe...Fe separations that change by less than 0.3 A upon the binding of additional bridging ligands. The structural constraints imposed by the protein host on the di-Fe cores are unique and create examples of active sites having entatic states within engineered artificial metalloproteins.
		+
		+	Artificial Metalloproteins with Dinuclear Iron-Hydroxido Centers.,Miller KR, Biswas S, Jasniewski A, Follmer AH, Biswas A, Albert T, Sabuncu S, Bominaar EL, Hendrich MP, Moenne-Loccoz P, Borovik AS J Am Chem Soc. 2021 Feb 10;143(5):2384-2393. doi: 10.1021/jacs.0c12564. Epub 2021 , Feb 2. PMID:33528256<ref>PMID:33528256</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6voz" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Avidin 3D structures\|Avidin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Streptomyces avidinii]]
	[[Category: Albert T]]		[[Category: Albert T]]
	[[Category: Biswas S]]		[[Category: Biswas S]]

OCA at 11:06, 3 February 2021

OCA — Wed, 03 Feb 2021 11:06:45 GMT

←Older revision		Revision as of 11:06, 3 February 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6voz is ~~ON HOLD until Paper Publication~~	+	==Artificial Metalloproteins with Dinuclear Iron Centers==
-		+	<StructureSection load='6voz' size='340' side='right'caption='[[6voz]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VOZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VOZ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6voz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6voz OCA], [https://pdbe.org/6voz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6voz RCSB], [https://www.ebi.ac.uk/pdbsum/6voz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6voz ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Albert T]]
		+	[[Category: Biswas S]]
		+	[[Category: Borovik AS]]
		+	[[Category: Follmer AH]]
		+	[[Category: Hendrich MP]]
		+	[[Category: Jasniewski AJ]]
		+	[[Category: Miller KR]]
		+	[[Category: Moenne-Loccoz P]]
		+	[[Category: Sabuncu S]]

OCA at 06:29, 22 July 2020

OCA — Wed, 22 Jul 2020 06:29:11 GMT

←Older revision		Revision as of 06:29, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6voz is ON HOLD	+	The entry 6voz is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 18:14, 24 June 2020

OCA — Wed, 24 Jun 2020 18:14:09 GMT

←Older revision		Revision as of 18:14, 24 June 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6voz is ON HOLD ~~until Paper Publication~~	+	The entry 6voz is ON HOLD

	Authors:		Authors:

OCA at 03:53, 13 May 2020

OCA — Wed, 13 May 2020 03:53:14 GMT

←Older revision		Revision as of 03:53, 13 May 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6voz is ON HOLD	+	The entry 6voz is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6voz" [edit=sysop:move=sysop]

OCA — Thu, 13 Feb 2020 02:43:20 GMT

Protected "6voz" [edit=sysop:move=sysop]

←Older revision

Revision as of 02:43, 13 February 2020

OCA: New page: '''Unreleased structure''' The entry 6voz is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Thu, 13 Feb 2020 02:43:18 GMT

New page: '''Unreleased structure''' The entry 6voz is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6voz is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]