6vvg - Revision history

OCA at 08:21, 11 October 2023

OCA — Wed, 11 Oct 2023 08:21:27 GMT

←Older revision		Revision as of 08:21, 11 October 2023
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	==Structure of the Cydia pomonella Granulovirus kinase, PK-1==		==Structure of the Cydia pomonella Granulovirus kinase, PK-1==
-	<StructureSection load='6vvg' size='340' side='right'caption='[[6vvg]]' scene=''>	+	<StructureSection load='6vvg' size='340' side='right'caption='[[6vvg]], [[Resolution\|resolution]] 2.01Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VVG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VVG FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6vvg]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Cydia_pomonella_granulosis_virus_(isolate_Mexican) Cydia pomonella granulosis virus (isolate Mexican)]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VVG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VVG FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vvg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vvg OCA], [https://pdbe.org/6vvg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vvg RCSB], [https://www.ebi.ac.uk/pdbsum/6vvg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vvg ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.01Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AMP:ADENOSINE+MONOPHOSPHATE'>AMP</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vvg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vvg OCA], [https://pdbe.org/6vvg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vvg RCSB], [https://www.ebi.ac.uk/pdbsum/6vvg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vvg ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A097P1P8_GVCP A0A097P1P8_GVCP]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The life cycle of Baculoviridae family insect viruses depends on the viral protein kinase, PK-1, to phosphorylate the regulatory protein, p6.9, to induce baculoviral genome release. Here, we report the crystal structure of Cydia pomenella granulovirus PK-1, which, owing to its likely ancestral origin among host cell AGC kinases, exhibits a eukaryotic protein kinase fold. PK-1 occurs as a rigid dimer, where an antiparallel arrangement of the alphaC helices at the dimer core stabilizes PK-1 in a closed, active conformation. Dimerization is facilitated by C-lobe:C-lobe and N-lobe:N-lobe interactions between protomers, including the domain-swapping of an N-terminal helix that crowns a contiguous beta-sheet formed by the two N-lobes. PK-1 retains a dimeric conformation in solution, which is crucial for catalytic activity. Our studies raise the prospect that parallel, side-to-side dimeric arrangements that lock kinase domains in a catalytically-active conformation could function more broadly as a regulatory mechanism among eukaryotic protein kinases.
		+
		+	Granulovirus PK-1 kinase activity relies on a side-to-side dimerization mode centered on the regulatory alphaC helix.,Oliver MR, Horne CR, Shrestha S, Keown JR, Liang LY, Young SN, Sandow JJ, Webb AI, Goldstone DC, Lucet IS, Kannan N, Metcalf P, Murphy JM Nat Commun. 2021 Feb 12;12(1):1002. doi: 10.1038/s41467-021-21191-7. PMID:33579933<ref>PMID:33579933</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6vvg" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Arginine kinase 3D structures\|Arginine kinase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 11:07, 3 February 2021

OCA — Wed, 03 Feb 2021 11:07:09 GMT

←Older revision		Revision as of 11:07, 3 February 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6vvg ~~is ON HOLD until Paper Publication~~	+	==Structure of the Cydia pomonella Granulovirus kinase, PK-1==
-		+	<StructureSection load='6vvg' size='340' side='right'caption='[[6vvg]]' scene=''>
-	~~Authors~~: ~~Oliver, M~~.R., ~~Horne, C~~.R., ~~Keown~~, J.R., ~~Murphy, J~~.M., ~~Metcalf, P~~.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VVG OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VVG FirstGlance]. <br>
-	~~Description: Structure of the Cydia pomonella Granulovirus arginine kinase, Pk-1~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vvg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vvg OCA], [https://pdbe.org/6vvg PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vvg RCSB], [https://www.ebi.ac.uk/pdbsum/6vvg PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vvg ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Keown, J.R~~]]	+	__TOC__
-	[[Category: ~~Murphy, J.M~~]]	+	</StructureSection>
-	[[Category: ~~Oliver, M.R~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Metcalf, P~~]]	+	[[Category: Horne CR]]
-	[[Category: ~~Horne, C.R~~]]	+	[[Category: Keown JR]]
		+	[[Category: Metcalf P]]
		+	[[Category: Murphy JM]]
		+	[[Category: Oliver MR]]

OCA at 05:07, 29 April 2020

OCA — Wed, 29 Apr 2020 05:07:49 GMT

←Older revision		Revision as of 05:07, 29 April 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6vvg is ON HOLD	+	The entry 6vvg is ON HOLD until Paper Publication

	Authors: Oliver, M.R., Horne, C.R., Keown, J.R., Murphy, J.M., Metcalf, P.		Authors: Oliver, M.R., Horne, C.R., Keown, J.R., Murphy, J.M., Metcalf, P.

OCA: Protected "6vvg" [edit=sysop:move=sysop]

OCA — Wed, 26 Feb 2020 08:11:58 GMT

Protected "6vvg" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:11, 26 February 2020

OCA: New page: '''Unreleased structure''' The entry 6vvg is ON HOLD Authors: Oliver, M.R., Horne, C.R., Keown, J.R., Murphy, J.M., Metcalf, P. Description: Structure of the Cydia pomonella Granulovir...

OCA — Wed, 26 Feb 2020 08:11:57 GMT

New page: '''Unreleased structure''' The entry 6vvg is ON HOLD Authors: Oliver, M.R., Horne, C.R., Keown, J.R., Murphy, J.M., Metcalf, P. Description: Structure of the Cydia pomonella Granulovir...

New page

'''Unreleased structure'''

The entry 6vvg is ON HOLD

Authors: Oliver, M.R., Horne, C.R., Keown, J.R., Murphy, J.M., Metcalf, P.

Description: Structure of the Cydia pomonella Granulovirus arginine kinase, Pk-1
[[Category: Unreleased Structures]]
[[Category: Keown, J.R]]
[[Category: Murphy, J.M]]
[[Category: Oliver, M.R]]
[[Category: Metcalf, P]]
[[Category: Horne, C.R]]