6vy4 - Revision history

OCA at 08:22, 11 October 2023

OCA — Wed, 11 Oct 2023 08:22:33 GMT

←Older revision		Revision as of 08:22, 11 October 2023
Line 1:		Line 1:

-	====	+	==Crystal structure of Hendra receptor binding protein head domain in complex with human neutralizing antibody HENV-32==
-	<StructureSection load='6vy4' size='340' side='right'caption='[[6vy4]]' scene=''>	+	<StructureSection load='6vy4' size='340' side='right'caption='[[6vy4]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6vy4]] is a 6 chain structure with sequence from [https://en.wikipedia.org/wiki/Hendra_henipavirus Hendra henipavirus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6VY4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6VY4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6vy4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vy4 OCA], [~~http~~://pdbe.org/6vy4 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6vy4 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6vy4 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6vy4 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=IPA:ISOPROPYL+ALCOHOL'>IPA</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6vy4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vy4 OCA], [https://pdbe.org/6vy4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6vy4 RCSB], [https://www.ebi.ac.uk/pdbsum/6vy4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6vy4 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/GLYCP_HENDH GLYCP_HENDH] Attaches the virus to sialic acid-containing cell receptors and thereby initiating infection. Binding of glycoprotein G to the receptor induces a conformational change that allows the F protein to trigger virion/cell membranes fusion (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Hendra (HeV) and Nipah (NiV) viruses are emerging zoonotic pathogens in the Henipavirus genus causing outbreaks of disease with very high case fatality rates. Here, we report the first naturally occurring human monoclonal antibodies (mAbs) against HeV receptor binding protein (RBP). All isolated mAbs neutralized HeV, and some also neutralized NiV. Epitope binning experiments identified five major antigenic sites on HeV-RBP. Animal studies demonstrated that the most potent cross-reactive neutralizing mAbs, HENV-26 and HENV-32, protected ferrets in lethal models of infection with NiV Bangladesh 3 days after exposure. We solved the crystal structures of mAb HENV-26 in complex with both HeV-RBP and NiV-RBP and of mAb HENV-32 in complex with HeV-RBP. The studies reveal diverse sites of vulnerability on RBP recognized by potent human mAbs that inhibit virus by multiple mechanisms. These studies identify promising prophylactic antibodies and define protective epitopes that can be used in rational vaccine design.
		+
		+	Potent Henipavirus Neutralization by Antibodies Recognizing Diverse Sites on Hendra and Nipah Virus Receptor Binding Protein.,Dong J, Cross RW, Doyle MP, Kose N, Mousa JJ, Annand EJ, Borisevich V, Agans KN, Sutton R, Nargi R, Majedi M, Fenton KA, Reichard W, Bombardi RG, Geisbert TW, Crowe JE Jr Cell. 2020 Dec 10;183(6):1536-1550.e17. doi: 10.1016/j.cell.2020.11.023. PMID:33306954<ref>PMID:33306954</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6vy4" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Hendra henipavirus]]
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Crowe JE]]
		+	[[Category: Dong J]]

OCA at 06:44, 30 December 2020

OCA — Wed, 30 Dec 2020 06:44:18 GMT

←Older revision		Revision as of 06:44, 30 December 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6vy4 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6vy4' size='340' side='right'caption='[[6vy4]]' scene=''>
-	~~Authors~~: ~~Dong, J~~.~~, Crowe, J~~.E.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Crystal structure of Hendra receptor binding protein head domain in complex with human neutralizing antibody HENV~~-32	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6vy4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6vy4 OCA], [http://pdbe.org/6vy4 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6vy4 RCSB], [http://www.ebi.ac.uk/pdbsum/6vy4 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6vy4 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Crowe, J.E~~]]	+	__TOC__
-	[[Category: ~~Dong, J~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 07:46, 18 November 2020

OCA — Wed, 18 Nov 2020 07:46:42 GMT

←Older revision		Revision as of 07:46, 18 November 2020
Line 5:		Line 5:
	Authors: Dong, J., Crowe, J.E.		Authors: Dong, J., Crowe, J.E.

-	Description: Crystal structure of Hendra ~~G attachment~~ protein head domain in complex with human neutralizing antibody HENV-32	+	Description: Crystal structure of Hendra receptor binding protein head domain in complex with human neutralizing antibody HENV-32
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Dong, J]]
	[[Category: Crowe, J.E]]		[[Category: Crowe, J.E]]
		+	[[Category: Dong, J]]

OCA: Protected "6vy4" [edit=sysop:move=sysop]

OCA — Wed, 04 Mar 2020 05:39:29 GMT

Protected "6vy4" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:39, 4 March 2020

OCA: New page: '''Unreleased structure''' The entry 6vy4 is ON HOLD until Paper Publication Authors: Dong, J., Crowe, J.E. Description: Crystal structure of Hendra G attachment protein head domain in...

OCA — Wed, 04 Mar 2020 05:39:28 GMT

New page: '''Unreleased structure''' The entry 6vy4 is ON HOLD until Paper Publication Authors: Dong, J., Crowe, J.E. Description: Crystal structure of Hendra G attachment protein head domain in...

New page

'''Unreleased structure'''

The entry 6vy4 is ON HOLD until Paper Publication

Authors: Dong, J., Crowe, J.E.

Description: Crystal structure of Hendra G attachment protein head domain in complex with human neutralizing antibody HENV-32
[[Category: Unreleased Structures]]
[[Category: Dong, J]]
[[Category: Crowe, J.E]]