6w0l - Revision history

OCA at 08:24, 11 October 2023

OCA — Wed, 11 Oct 2023 08:24:10 GMT

←Older revision		Revision as of 08:24, 11 October 2023
Line 3:		Line 3:
	<StructureSection load='6w0l' size='340' side='right'caption='[[6w0l]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='6w0l' size='340' side='right'caption='[[6w0l]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6w0l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Black_flying_fox Black flying fox~~] and [https://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W0L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6W0L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6w0l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Pteropus_alecto Pteropus alecto]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W0L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6W0L FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.3Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=~~SEP~~:~~PHOSPHOSERINE~~'>~~SEP~~</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b~~></td>~~<td class="sblockDat">SFN, HME1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])~~</td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w0l OCA], [https://pdbe.org/6w0l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w0l RCSB], [https://www.ebi.ac.uk/pdbsum/6w0l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w0l ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w0l OCA], [https://pdbe.org/6w0l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w0l RCSB], [https://www.ebi.ac.uk/pdbsum/6w0l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w0l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/1433S_HUMAN 1433S_HUMAN]] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). p53-regulated inhibitor of G2/M progression.	+	[https://www.uniprot.org/uniprot/1433S_HUMAN 1433S_HUMAN] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). p53-regulated inhibitor of G2/M progression.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 27:		Line 26:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Black flying fox]]~~	+	[[Category: Homo sapiens]]
-	~~[[Category: Human~~]]	+
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Basler, C]]	+	[[Category: Pteropus alecto]]
-	[[Category: Edwards, M]]	+	[[Category: Basler C]]
-	[[Category: Forwood, J]]	+	[[Category: Edwards M]]
-	[[Category: Hoad, M]]	+	[[Category: Forwood J]]
-	[[Category: Menicucci, A]]	+	[[Category: Hoad M]]
-	[[Category: Messaoudi, I]]	+	[[Category: Menicucci A]]
-	[[Category: Tsimbalyuk, S]]	+	[[Category: Messaoudi I]]
-	~~[[Category: Complex]]~~	+	[[Category: Tsimbalyuk S]]
-	~~[[Category: Nuclear import]]~~	+
-	~~[[Category: Nuclear localization]]~~	+
-	~~[[Category: Phosphorylation]]~~	+
-	~~[[Category: Protein binding~~]]	+

OCA at 08:58, 5 May 2021

OCA — Wed, 05 May 2021 08:58:59 GMT

←Older revision		Revision as of 08:58, 5 May 2021
Line 3:		Line 3:
	<StructureSection load='6w0l' size='340' side='right'caption='[[6w0l]], [[Resolution\|resolution]] 2.30Å' scene=''>		<StructureSection load='6w0l' size='340' side='right'caption='[[6w0l]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6w0l]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Black_flying_fox Black flying fox] and [~~http~~://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W0L OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6W0L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6w0l]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Black_flying_fox Black flying fox] and [https://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W0L OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6W0L FirstGlance]. <br>
	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>		</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>		<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
-	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SFN, HME1 ([~~http~~://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>	+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SFN, HME1 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6w0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w0l OCA], [~~http~~://pdbe.org/6w0l PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6w0l RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6w0l PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6w0l ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w0l OCA], [https://pdbe.org/6w0l PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w0l RCSB], [https://www.ebi.ac.uk/pdbsum/6w0l PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w0l ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/1433S_HUMAN 1433S_HUMAN]] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). p53-regulated inhibitor of G2/M progression.	+	[[https://www.uniprot.org/uniprot/1433S_HUMAN 1433S_HUMAN]] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). p53-regulated inhibitor of G2/M progression.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 20:
	</div>		</div>
	<div class="pdbe-citations 6w0l" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6w0l" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[14-3-3 protein 3D structures\|14-3-3 protein 3D structures]]
	== References ==		== References ==
	<references/>		<references/>

OCA at 06:42, 6 May 2020

OCA — Wed, 06 May 2020 06:42:55 GMT

←Older revision		Revision as of 06:42, 6 May 2020
Line 1:		Line 1:

	==Henipavirus W protein interacts with 14-3-3 to modulate host gene expression==		==Henipavirus W protein interacts with 14-3-3 to modulate host gene expression==
-	<StructureSection load='6w0l' size='340' side='right'caption='[[6w0l]]' scene=''>	+	<StructureSection load='6w0l' size='340' side='right'caption='[[6w0l]], [[Resolution\|resolution]] 2.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W0L OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6W0L FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6w0l]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Black_flying_fox Black flying fox] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W0L OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6W0L FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6w0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w0l OCA], [http://pdbe.org/6w0l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6w0l RCSB], [http://www.ebi.ac.uk/pdbsum/6w0l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6w0l ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=SEP:PHOSPHOSERINE'>SEP</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SFN, HME1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6w0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w0l OCA], [http://pdbe.org/6w0l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6w0l RCSB], [http://www.ebi.ac.uk/pdbsum/6w0l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6w0l ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/1433S_HUMAN 1433S_HUMAN]] Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway (By similarity). p53-regulated inhibitor of G2/M progression.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nipah (NiV) and Hendra virus (HeV), members of the henipavirus genus in the Paramyxoviridae family, are recently emerged, highly lethal zoonotic pathogens. The NiV and HeV non-segmented, negative-sense RNA genomes encode for nine proteins, including the W protein. Expressed from the P gene through mRNA editing, W shares a common N-terminus with P and V, but has a unique C-terminus. Expressed alone, W modulates innate immune responses by several mechanisms and elimination of W from NiV alters the course of infection in experimentally-infected-ferrets. However, the specific host interactions that allow W to modulate innate immunity are incompletely understood. This study demonstrates that the NiV and HeV W proteins interact with all seven isoforms of the 14-3-3 family, regulatory molecules that preferentially bind phosphorylated target proteins to regulate a wide range of cellular functions. The interaction is dependent on the penultimate amino acid residue in the W sequence, a conserved, phosphorylated serine. The co-crystal structure of the W C-terminal binding motif with 14-3-3 provides only the second structure of a complex containing a mode III interactor, which is defined as a 14-3-3 interaction with a phosphoserine/phosphothreonine at the C-termini of the target protein. Transcriptomic analysis of inducible cell lines infected with an RNA virus and expressing either wildtype W or W lacking 14-3-3 binding, identifies new functions for W. These include the regulation of cellular metabolic processes, cell junction organization and apoptosis.IMPORTANCE Nipah (NiV) and Hendra virus (HeV), members of the Henipavirus genus, are recently emerged, highly lethal zoonotic pathogens that cause yearly outbreaks. NiV and HeV each encode a W protein that has roles in regulating host signaling pathways, including antagonism of the innate immune response. However, the mechanisms used by W to regulate these host responses are not clear. Here, characterization of the interaction of NiV and HeV W with 14-3-3 identifies modulation of 14-3-3 regulated host signaling pathways not previously associated with W, suggesting new avenues of research. The co-crystal structure of the NiV W:14-3-3 complex, as only the second structure of a 14-3-3 mode III interactor, provides further insight into this less well understood 14-3-3 binding motif.
		+
		+	Henipavirus W proteins interact with 14-3-3 to modulate host gene expression.,Edwards MR, Hoad M, Tsimbalyuk S, Menicucci AR, Messaoudi I, Forwood JK, Basler CF J Virol. 2020 Apr 22. pii: JVI.00373-20. doi: 10.1128/JVI.00373-20. PMID:32321809<ref>PMID:32321809</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6w0l" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Black flying fox]]
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Basler C]]	+	[[Category: Basler, C]]
-	[[Category: Edwards M]]	+	[[Category: Edwards, M]]
-	[[Category: Forwood J]]	+	[[Category: Forwood, J]]
-	[[Category: Hoad M]]	+	[[Category: Hoad, M]]
-	[[Category: Menicucci A]]	+	[[Category: Menicucci, A]]
-	[[Category: Messaoudi I]]	+	[[Category: Messaoudi, I]]
-	[[Category: Tsimbalyuk S]]	+	[[Category: Tsimbalyuk, S]]
		+	[[Category: Complex]]
		+	[[Category: Nuclear import]]
		+	[[Category: Nuclear localization]]
		+	[[Category: Phosphorylation]]
		+	[[Category: Protein binding]]

OCA at 05:47, 22 April 2020

OCA — Wed, 22 Apr 2020 05:47:49 GMT

←Older revision		Revision as of 05:47, 22 April 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry 6w0l is ON HOLD~~	+	==Henipavirus W protein interacts with 14-3-3 to modulate host gene expression==
-		+	<StructureSection load='6w0l' size='340' side='right'caption='[[6w0l]]' scene=''>
-	~~Authors: Edwards, M., Hoad, M., Tsimbalyuk, S., Menicucci, A., Messaoudi, I., Forwood, J., Basler, C.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W0L OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6W0L FirstGlance]. <br>
-	~~Description:~~ Henipavirus W protein interacts with 14-3-3 to modulate host gene expression	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6w0l FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w0l OCA], [http://pdbe.org/6w0l PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6w0l RCSB], [http://www.ebi.ac.uk/pdbsum/6w0l PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6w0l ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[~~Category~~: ~~Edwards~~, M]]	+	__TOC__
-	[[Category: ~~Forwood, J~~]]	+	</StructureSection>
-	[[Category: ~~Tsimbalyuk, S~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Basler, C~~]]	+	[[Category: Basler C]]
-	[[Category: Hoad, M]]	+	[[Category: Edwards M]]
-	[[Category: Menicucci, A]]	+	[[Category: Forwood J]]
-	[[Category: Messaoudi, I]]	+	[[Category: Hoad M]]
		+	[[Category: Menicucci A]]
		+	[[Category: Messaoudi I]]
		+	[[Category: Tsimbalyuk S]]

OCA: Protected "6w0l" [edit=sysop:move=sysop]

OCA — Wed, 11 Mar 2020 06:07:14 GMT

Protected "6w0l" [edit=sysop:move=sysop]

←Older revision

Revision as of 06:07, 11 March 2020

OCA: New page: '''Unreleased structure''' The entry 6w0l is ON HOLD Authors: Edwards, M., Hoad, M., Tsimbalyuk, S., Menicucci, A., Messaoudi, I., Forwood, J., Basler, C. Description: Henipavirus W pr...

OCA — Wed, 11 Mar 2020 06:07:12 GMT

New page: '''Unreleased structure''' The entry 6w0l is ON HOLD Authors: Edwards, M., Hoad, M., Tsimbalyuk, S., Menicucci, A., Messaoudi, I., Forwood, J., Basler, C. Description: Henipavirus W pr...

New page

'''Unreleased structure'''

The entry 6w0l is ON HOLD

Authors: Edwards, M., Hoad, M., Tsimbalyuk, S., Menicucci, A., Messaoudi, I., Forwood, J., Basler, C.

Description: Henipavirus W protein interacts with 14-3-3 to modulate host gene expression
[[Category: Unreleased Structures]]
[[Category: Edwards, M]]
[[Category: Forwood, J]]
[[Category: Tsimbalyuk, S]]
[[Category: Basler, C]]
[[Category: Hoad, M]]
[[Category: Menicucci, A]]
[[Category: Messaoudi, I]]