6w4v - Revision history

OCA at 14:16, 18 October 2023

OCA — Wed, 18 Oct 2023 14:16:54 GMT

←Older revision		Revision as of 14:16, 18 October 2023
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-	====	+	==Structure of anti-ferroportin Fab45D8==
-	<StructureSection load='6w4v' size='340' side='right'caption='[[6w4v]]' scene=''>	+	<StructureSection load='6w4v' size='340' side='right'caption='[[6w4v]], [[Resolution\|resolution]] 2.10Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6w4v]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W4V OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6W4V FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6w4v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w4v OCA], [~~http~~://pdbe.org/6w4v PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6w4v RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6w4v PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6w4v ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.1Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w4v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w4v OCA], [https://pdbe.org/6w4v PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w4v RCSB], [https://www.ebi.ac.uk/pdbsum/6w4v PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w4v ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The serum iron level in humans is tightly controlled by the action of the hormone hepcidin on the iron efflux transporter ferroportin. Hepcidin regulates iron absorption and recycling by inducing ferroportin internalization and degradation(1). Aberrant ferroportin activity can lead to diseases of iron overload, such as hemochromatosis, or iron limitation anemias(2). Here, we determined cryogenic electron microscopy (cryo-EM) structures of ferroportin in lipid nanodiscs, both in the apo state and in complex with cobalt, an iron mimetic, and hepcidin. These structures and accompanying molecular dynamics simulations identify two metal binding sites within the N- and C-domains of ferroportin. Hepcidin binds ferroportin in an outward-open conformation and completely occludes the iron efflux pathway to inhibit transport. The carboxy-terminus of hepcidin directly contacts the divalent metal in the ferroportin C-domain. We further show that hepcidin binding to ferroportin is coupled to iron binding, with an 80-fold increase in hepcidin affinity in the presence of iron. These results suggest a model for hepcidin regulation of ferroportin, where only iron loaded ferroportin molecules are targeted for degradation. More broadly, our structural and functional insights are likely to enable more targeted manipulation of the hepcidin-ferroportin axis in disorders of iron homeostasis.
		+
		+	Structure of hepcidin-bound ferroportin reveals iron homeostatic mechanisms.,Billesbolle CB, Azumaya CM, Kretsch RC, Powers AS, Gonen S, Schneider S, Arvedson T, Dror RO, Cheng Y, Manglik A Nature. 2020 Aug 19. pii: 10.1038/s41586-020-2668-z. doi:, 10.1038/s41586-020-2668-z. PMID:32814342<ref>PMID:32814342</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6w4v" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Mus musculus]]
		+	[[Category: Arvedson T]]
		+	[[Category: Azumaya CM]]
		+	[[Category: Billesboelle CB]]
		+	[[Category: Cheng Y]]
		+	[[Category: Dror RO]]
		+	[[Category: Gonen S]]
		+	[[Category: Kretsch RC]]
		+	[[Category: Manglik A]]
		+	[[Category: Powers A]]
		+	[[Category: Schneider S]]

OCA at 09:33, 9 September 2020

OCA — Wed, 09 Sep 2020 09:33:25 GMT

←Older revision		Revision as of 09:33, 9 September 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6w4v ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6w4v' size='340' side='right'caption='[[6w4v]]' scene=''>
-	~~Authors~~: ~~Billesboelle, C~~.B.~~, Azumaya, C~~.M.~~, Gonen, S~~.~~, Powers, A~~.~~, Kretsch, R~~.C.~~, Schneider, S~~., ~~Arvedson, T~~.~~, Dror, R~~.O.~~, Cheng, Y., Manglik, A.~~	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Structure of anti~~-~~ferroportin Fab45D8~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6w4v FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w4v OCA], [http://pdbe.org/6w4v PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6w4v RCSB], [http://www.ebi.ac.uk/pdbsum/6w4v PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6w4v ProSAT]</span></td></tr>
-	~~[[Category: Unreleased Structures~~]]	+	</table>
-	[~~[Category~~: ~~Kretsch, R~~.C]]	+	__TOC__
-	[[~~Category~~: ~~Cheng, Y~~]]	+	</StructureSection>
-	~~[[Category: Manglik~~, ~~A]]~~	+	[[Category: Large Structures]]
-	[~~[Category~~: ~~Azumaya, C~~.M]]	+	[[Category: Z-disk]]
-	[~~[Category~~: ~~Dror, R~~.O]]	+
-	~~[[Category: Arvedson, T]]~~	+
-	~~[[Category: Powers, A]]~~	+
-	~~[[Category: Gonen, S]]~~	+
-	[[Category: ~~Billesboelle, C.B~~]]	+
-	[[Category: ~~Schneider, S~~]]	+

OCA at 05:33, 8 April 2020

OCA — Wed, 08 Apr 2020 05:33:17 GMT

←Older revision		Revision as of 05:33, 8 April 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6w4v is ON HOLD	+	The entry 6w4v is ON HOLD until Paper Publication

	Authors: Billesboelle, C.B., Azumaya, C.M., Gonen, S., Powers, A., Kretsch, R.C., Schneider, S., Arvedson, T., Dror, R.O., Cheng, Y., Manglik, A.		Authors: Billesboelle, C.B., Azumaya, C.M., Gonen, S., Powers, A., Kretsch, R.C., Schneider, S., Arvedson, T., Dror, R.O., Cheng, Y., Manglik, A.

OCA: Protected "6w4v" [edit=sysop:move=sysop]

OCA — Wed, 18 Mar 2020 08:35:37 GMT

Protected "6w4v" [edit=sysop:move=sysop]

←Older revision

Revision as of 08:35, 18 March 2020

OCA: New page: '''Unreleased structure''' The entry 6w4v is ON HOLD Authors: Billesboelle, C.B., Azumaya, C.M., Gonen, S., Powers, A., Kretsch, R.C., Schneider, S., Arvedson, T., Dror, R.O., Cheng, Y....

OCA — Wed, 18 Mar 2020 08:35:35 GMT

New page: '''Unreleased structure''' The entry 6w4v is ON HOLD Authors: Billesboelle, C.B., Azumaya, C.M., Gonen, S., Powers, A., Kretsch, R.C., Schneider, S., Arvedson, T., Dror, R.O., Cheng, Y....

New page

'''Unreleased structure'''

The entry 6w4v is ON HOLD

Authors: Billesboelle, C.B., Azumaya, C.M., Gonen, S., Powers, A., Kretsch, R.C., Schneider, S., Arvedson, T., Dror, R.O., Cheng, Y., Manglik, A.

Description: Structure of anti-ferroportin Fab45D8
[[Category: Unreleased Structures]]
[[Category: Kretsch, R.C]]
[[Category: Cheng, Y]]
[[Category: Manglik, A]]
[[Category: Azumaya, C.M]]
[[Category: Dror, R.O]]
[[Category: Arvedson, T]]
[[Category: Powers, A]]
[[Category: Gonen, S]]
[[Category: Billesboelle, C.B]]
[[Category: Schneider, S]]