OCA at 14:18, 18 October 2023

OCA — Wed, 18 Oct 2023 14:18:32 GMT

←Older revision		Revision as of 14:18, 18 October 2023
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	==K2P2.1 (TREK-1):ML335 complex, 30 mM K+==		==K2P2.1 (TREK-1):ML335 complex, 30 mM K+==
-	<StructureSection load='6w88' size='340' side='right'caption='[[6w88]]' scene=''>	+	<StructureSection load='6w88' size='340' side='right'caption='[[6w88]], [[Resolution\|resolution]] 3.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W88 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6W88 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6w88]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W88 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6W88 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w88 OCA], [https://pdbe.org/6w88 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w88 RCSB], [https://www.ebi.ac.uk/pdbsum/6w88 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w88 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 3.2Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CD:CADMIUM+ION'>CD</scene>, <scene name='pdbligand=D10:DECANE'>D10</scene>, <scene name='pdbligand=D12:DODECANE'>D12</scene>, <scene name='pdbligand=K:POTASSIUM+ION'>K</scene>, <scene name='pdbligand=LNK:PENTANE'>LNK</scene>, <scene name='pdbligand=OCT:N-OCTANE'>OCT</scene>, <scene name='pdbligand=Q6F:N-[(2,4-dichlorophenyl)methyl]-4-[(methylsulfonyl)amino]benzamide'>Q6F</scene>, <scene name='pdbligand=R16:HEXADECANE'>R16</scene>, <scene name='pdbligand=UND:UNDECANE'>UND</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w88 OCA], [https://pdbe.org/6w88 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w88 RCSB], [https://www.ebi.ac.uk/pdbsum/6w88 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w88 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/KCNK2_MOUSE KCNK2_MOUSE] Ion channel that contributes to passive transmembrane potassium transport. Reversibly converts between a voltage-insensitive potassium leak channel and a voltage-dependent outward rectifying potassium channel in a phosphorylation-dependent manner. In astrocytes, forms mostly heterodimeric potassium channels with KCNK1, with only a minor proportion of functional channels containing homodimeric KCNK2 (PubMed:24496152). In astrocytes, the heterodimer formed by KCNK1 and KCNK2 is required for rapid glutamate release in response to activation of G-protein coupled receptors, such as F2R and CNR1 (PubMed:24496152).<ref>PMID:10321245</ref> <ref>PMID:16636285</ref> <ref>PMID:24496152</ref> <ref>PMID:9003761</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	K2P potassium channels regulate cellular excitability using their selectivity filter (C-type) gate. C-type gating mechanisms, best characterized in homotetrameric potassium channels, remain controversial and are attributed to selectivity filter pinching, dilation, or subtle structural changes. The extent to which such mechanisms control C-type gating of innately heterodimeric K2Ps is unknown. Here, combining K2P2.1 (TREK-1) x-ray crystallography in different potassium concentrations, potassium anomalous scattering, molecular dynamics, and electrophysiology, we uncover unprecedented, asymmetric, potassium-dependent conformational changes that underlie K2P C-type gating. These asymmetric order-disorder transitions, enabled by the K2P heterodimeric architecture, encompass pinching and dilation, disrupt the S1 and S2 ion binding sites, require the uniquely long K2P SF2-M4 loop and conserved "M3 glutamate network," and are suppressed by the K2P C-type gate activator ML335. These findings demonstrate that two distinct C-type gating mechanisms can operate in one channel and underscore the SF2-M4 loop as a target for K2P channel modulator development.
		+
		+	K2P channel C-type gating involves asymmetric selectivity filter order-disorder transitions.,Lolicato M, Natale AM, Abderemane-Ali F, Crottes D, Capponi S, Duman R, Wagner A, Rosenberg JM, Grabe M, Minor DL Jr Sci Adv. 2020 Oct 30;6(44). pii: 6/44/eabc9174. doi: 10.1126/sciadv.abc9174., Print 2020 Oct. PMID:33127683<ref>PMID:33127683</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6w88" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Potassium channel 3D structures\|Potassium channel 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Mus musculus]]
	[[Category: Lolicato M]]		[[Category: Lolicato M]]
	[[Category: Minor DL]]		[[Category: Minor DL]]

OCA at 07:54, 27 January 2021

OCA — Wed, 27 Jan 2021 07:54:40 GMT

←Older revision		Revision as of 07:54, 27 January 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6w88 ~~is ON HOLD until Paper Publication~~	+	==K2P2.1 (TREK-1):ML335 complex, 30 mM K+==
-		+	<StructureSection load='6w88' size='340' side='right'caption='[[6w88]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6W88 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6W88 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6w88 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6w88 OCA], [https://pdbe.org/6w88 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6w88 RCSB], [https://www.ebi.ac.uk/pdbsum/6w88 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6w88 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Lolicato M]]
		+	[[Category: Minor DL]]

OCA at 05:33, 8 April 2020

OCA — Wed, 08 Apr 2020 05:33:58 GMT

←Older revision		Revision as of 05:33, 8 April 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6w88 is ON HOLD	+	The entry 6w88 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6w88" [edit=sysop:move=sysop]

OCA — Wed, 01 Apr 2020 07:27:00 GMT

Protected "6w88" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:27, 1 April 2020

OCA: New page: '''Unreleased structure''' The entry 6w88 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 01 Apr 2020 07:26:58 GMT

New page: '''Unreleased structure''' The entry 6w88 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6w88 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6w88 - Revision history

OCA at 14:18, 18 October 2023

OCA at 07:54, 27 January 2021

OCA at 05:33, 8 April 2020

OCA: Protected "6w88" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6w88 is ON HOLD Authors: Description: Category: Unreleased Structures