OCA at 09:59, 8 December 2021

OCA — Wed, 08 Dec 2021 09:59:30 GMT

←Older revision		Revision as of 09:59, 8 December 2021
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	==Microbiome-derived Acarbose Kinase Mak1 Labeled with selenomethionine==		==Microbiome-derived Acarbose Kinase Mak1 Labeled with selenomethionine==
-	<StructureSection load='6wb4' size='340' side='right'caption='[[6wb4]]' scene=''>	+	<StructureSection load='6wb4' size='340' side='right'caption='[[6wb4]], [[Resolution\|resolution]] 2.59Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WB4 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wb4]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Uncultivated_bacterium Uncultivated bacterium]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WB4 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wb4 OCA], [https://pdbe.org/6wb4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wb4 RCSB], [https://www.ebi.ac.uk/pdbsum/6wb4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wb4 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ATP:ADENOSINE-5-TRIPHOSPHATE'>ATP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=AC1:6-METHYL-5-(4,5,6-TRIHYDROXY-3-HYDROXYMETHYL-CYCLOHEX-2-ENYLAMINO)-TETRAHYDRO-PYRAN-2,3,4-TRIOL'>AC1</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wb4 OCA], [https://pdbe.org/6wb4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wb4 RCSB], [https://www.ebi.ac.uk/pdbsum/6wb4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wb4 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The human microbiome encodes a large repertoire of biochemical enzymes and pathways, most of which remain uncharacterized. Here, using a metagenomics-based search strategy, we discovered that bacterial members of the human gut and oral microbiome encode enzymes that selectively phosphorylate a clinically used antidiabetic drug, acarbose(1,2), resulting in its inactivation. Acarbose is an inhibitor of both human and bacterial alpha-glucosidases(3), limiting the ability of the target organism to metabolize complex carbohydrates. Using biochemical assays, X-ray crystallography and metagenomic analyses, we show that microbiome-derived acarbose kinases are specific for acarbose, provide their harbouring organism with a protective advantage against the activity of acarbose, and are widespread in the microbiomes of western and non-western human populations. These results provide an example of widespread microbiome resistance to a non-antibiotic drug, and suggest that acarbose resistance has disseminated in the human microbiome as a defensive strategy against a potential endogenous producer of a closely related molecule.
		+
		+	The human microbiome encodes resistance to the antidiabetic drug acarbose.,Balaich J, Estrella M, Wu G, Jeffrey PD, Biswas A, Zhao L, Korennykh A, Donia MS Nature. 2021 Dec;600(7887):110-115. doi: 10.1038/s41586-021-04091-0. Epub 2021, Nov 24. PMID:34819672<ref>PMID:34819672</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6wb4" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Balaich JN]]	+	[[Category: Uncultivated bacterium]]
-	[[Category: Donia MS]]	+	[[Category: Balaich, J N]]
-	[[Category: Estrella MA]]	+	[[Category: Donia, M S]]
-	[[Category: Jeffrey PD]]	+	[[Category: Estrella, M A]]
		+	[[Category: Jeffrey, P D]]
		+	[[Category: Acarbose]]
		+	[[Category: Atp binding]]
		+	[[Category: Carbohydrate kinase]]
		+	[[Category: Complex]]
		+	[[Category: Metal ion binding]]
		+	[[Category: Nucleotide binding]]
		+	[[Category: Ribokinase activity]]
		+	[[Category: Transferase]]

OCA at 08:19, 21 April 2021

OCA — Wed, 21 Apr 2021 08:19:25 GMT

←Older revision		Revision as of 08:19, 21 April 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6wb4 is ~~ON HOLD until Paper Publication~~	+	==Microbiome-derived Acarbose Kinase Mak1 Labeled with selenomethionine==
-		+	<StructureSection load='6wb4' size='340' side='right'caption='[[6wb4]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WB4 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WB4 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wb4 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wb4 OCA], [https://pdbe.org/6wb4 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wb4 RCSB], [https://www.ebi.ac.uk/pdbsum/6wb4 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wb4 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Balaich JN]]
		+	[[Category: Donia MS]]
		+	[[Category: Estrella MA]]
		+	[[Category: Jeffrey PD]]

OCA at 03:59, 13 May 2020

OCA — Wed, 13 May 2020 03:59:39 GMT

←Older revision		Revision as of 03:59, 13 May 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6wb4 is ON HOLD	+	The entry 6wb4 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6wb4" [edit=sysop:move=sysop]

OCA — Wed, 01 Apr 2020 07:36:15 GMT

Protected "6wb4" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:36, 1 April 2020

OCA: New page: '''Unreleased structure''' The entry 6wb4 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 01 Apr 2020 07:36:13 GMT

New page: '''Unreleased structure''' The entry 6wb4 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6wb4 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6wb4 - Revision history

OCA at 09:59, 8 December 2021

OCA at 08:19, 21 April 2021

OCA at 03:59, 13 May 2020

OCA: Protected "6wb4" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6wb4 is ON HOLD Authors: Description: Category: Unreleased Structures