6wb7 - Revision history

OCA at 14:20, 18 October 2023

OCA — Wed, 18 Oct 2023 14:20:28 GMT

←Older revision		Revision as of 14:20, 18 October 2023
Line 3:		Line 3:
	<StructureSection load='6wb7' size='340' side='right'caption='[[6wb7]], [[Resolution\|resolution]] 2.44Å' scene=''>		<StructureSection load='6wb7' size='340' side='right'caption='[[6wb7]], [[Resolution\|resolution]] 2.44Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6wb7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/~~Acts5 Acts5~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WB7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wb7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Actinoplanes_sp._SE50/110 Actinoplanes sp. SE50/110]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WB7 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID~~-~~ADENYLATE+ESTER'>ANP</scene>~~, ~~<scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.44Å</td></tr>
-	<tr id='~~NonStdRes~~'><td class="sblockLbl"><b>[[~~Non-Standard_Residue~~\|~~NonStd Res~~:]]</b></td><td class="sblockDat"><scene name='pdbligand=AC1:6-METHYL-5-(4,5,6-TRIHYDROXY-3-HYDROXYMETHYL-CYCLOHEX-2-ENYLAMINO)-TETRAHYDRO-PYRAN-2,3,4-TRIOL'>AC1</scene><~~/td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=AC1:6-METHYL-5-(4,5,6-TRIHYDROXY-3-HYDROXYMETHYL-CYCLOHEX-2-ENYLAMINO)-TETRAHYDRO-PYRAN-2,3,4-TRIOL'>AC1</scene>, <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=PRD_900007:alpha-acarbose'>PRD_900007</scene></td></tr>
-	~~<tr id~~='~~related'><td class~~=~~"sblockLbl"><b>[[Related_structure\|Related~~:~~]]</b~~></td><~~td class~~=~~"sblockDat"><div style~~=~~'overflow~~: ~~auto; max~~-~~height: 3em;~~'>~~[[6wb4\|6wb4]], [[6wb5\|6wb5]]~~</~~div~~><~~/td></tr>~~	+
-	~~<tr id~~='~~gene'><td class~~=~~"sblockLbl"><b>[[Gene\|Gene~~:~~]]</b></td><td class="sblockDat">acbK, ACPL_3675~~ (~~[https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=134676 ACTS5]~~)~~</td~~></tr>	+
-	<~~tr id~~='~~activity'><td class~~=~~"sblockLbl"><b>Activity~~:~~</b~~></td><~~td class~~=~~"sblockDat"><span class~~=~~'plainlinks~~'>~~[https://en.wikipedia.org/wiki/Acarbose_7(IV)-phosphotransferase Acarbose 7(IV)-phosphotransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.187 2.7.1.187]~~ </~~span~~></td></tr>	+
	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wb7 OCA], [https://pdbe.org/6wb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wb7 RCSB], [https://www.ebi.ac.uk/pdbsum/6wb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wb7 ProSAT]</span></td></tr>		<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wb7 OCA], [https://pdbe.org/6wb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wb7 RCSB], [https://www.ebi.ac.uk/pdbsum/6wb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wb7 ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[[https://www.uniprot.org/uniprot/ACBK_ACTS5 ACBK_ACTS5]] Catalyzes the phosphorylation of the alpha-glucosidase inhibitor acarbose. Phosphorylation of acarbose could be a resistance-like self-protection mechanism.<ref>PMID:11937512</ref>	+	[https://www.uniprot.org/uniprot/ACBK_ACTS5 ACBK_ACTS5] Catalyzes the phosphorylation of the alpha-glucosidase inhibitor acarbose. Phosphorylation of acarbose could be a resistance-like self-protection mechanism.<ref>PMID:11937512</ref>
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 26:		Line 23:
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Acts5~~]]	+	[[Category: Actinoplanes sp. SE50/110]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Balaich~~, J N~~]]	+	[[Category: Balaich JN]]
-	[[Category: Donia~~, M S~~]]	+	[[Category: Donia MS]]
-	[[Category: Estrella~~, M A~~]]	+	[[Category: Estrella MA]]
-	[[Category: Jeffrey~~, P D]]~~	+	[[Category: Jeffrey PD]]
-	~~[[Category: Acarbose]]~~	+
-	~~[[Category: Atp binding]]~~	+
-	~~[[Category: Carbohydrate kinase]]~~	+
-	~~[[Category: Complex]]~~	+
-	~~[[Category: Metal ion binding]]~~	+
-	~~[[Category: Nucleotide binding]]~~	+
-	~~[[Category: Ribokinase activity]]~~	+
-	~~[[Category: Transferase~~]]	+

OCA at 09:59, 8 December 2021

OCA — Wed, 08 Dec 2021 09:59:37 GMT

←Older revision		Revision as of 09:59, 8 December 2021
Line 1:		Line 1:

	==Acarbose Kinase AcbK as a Complex with Acarbose and AMP-PNP==		==Acarbose Kinase AcbK as a Complex with Acarbose and AMP-PNP==
-	<StructureSection load='6wb7' size='340' side='right'caption='[[6wb7]]' scene=''>	+	<StructureSection load='6wb7' size='340' side='right'caption='[[6wb7]], [[Resolution\|resolution]] 2.44Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WB7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wb7]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Acts5 Acts5]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WB7 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wb7 OCA], [https://pdbe.org/6wb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wb7 RCSB], [https://www.ebi.ac.uk/pdbsum/6wb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wb7 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=AC1:6-METHYL-5-(4,5,6-TRIHYDROXY-3-HYDROXYMETHYL-CYCLOHEX-2-ENYLAMINO)-TETRAHYDRO-PYRAN-2,3,4-TRIOL'>AC1</scene></td></tr>
		+	<tr id='related'><td class="sblockLbl"><b>[[Related_structure\|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[6wb4\|6wb4]], [[6wb5\|6wb5]]</div></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">acbK, ACPL_3675 ([https://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=134676 ACTS5])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[https://en.wikipedia.org/wiki/Acarbose_7(IV)-phosphotransferase Acarbose 7(IV)-phosphotransferase], with EC number [https://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.1.187 2.7.1.187] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wb7 OCA], [https://pdbe.org/6wb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wb7 RCSB], [https://www.ebi.ac.uk/pdbsum/6wb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wb7 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[https://www.uniprot.org/uniprot/ACBK_ACTS5 ACBK_ACTS5]] Catalyzes the phosphorylation of the alpha-glucosidase inhibitor acarbose. Phosphorylation of acarbose could be a resistance-like self-protection mechanism.<ref>PMID:11937512</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The human microbiome encodes a large repertoire of biochemical enzymes and pathways, most of which remain uncharacterized. Here, using a metagenomics-based search strategy, we discovered that bacterial members of the human gut and oral microbiome encode enzymes that selectively phosphorylate a clinically used antidiabetic drug, acarbose(1,2), resulting in its inactivation. Acarbose is an inhibitor of both human and bacterial alpha-glucosidases(3), limiting the ability of the target organism to metabolize complex carbohydrates. Using biochemical assays, X-ray crystallography and metagenomic analyses, we show that microbiome-derived acarbose kinases are specific for acarbose, provide their harbouring organism with a protective advantage against the activity of acarbose, and are widespread in the microbiomes of western and non-western human populations. These results provide an example of widespread microbiome resistance to a non-antibiotic drug, and suggest that acarbose resistance has disseminated in the human microbiome as a defensive strategy against a potential endogenous producer of a closely related molecule.
		+
		+	The human microbiome encodes resistance to the antidiabetic drug acarbose.,Balaich J, Estrella M, Wu G, Jeffrey PD, Biswas A, Zhao L, Korennykh A, Donia MS Nature. 2021 Dec;600(7887):110-115. doi: 10.1038/s41586-021-04091-0. Epub 2021, Nov 24. PMID:34819672<ref>PMID:34819672</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6wb7" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Acts5]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Balaich JN]]	+	[[Category: Balaich, J N]]
-	[[Category: Donia MS]]	+	[[Category: Donia, M S]]
-	[[Category: Estrella MA]]	+	[[Category: Estrella, M A]]
-	[[Category: Jeffrey PD]]	+	[[Category: Jeffrey, P D]]
		+	[[Category: Acarbose]]
		+	[[Category: Atp binding]]
		+	[[Category: Carbohydrate kinase]]
		+	[[Category: Complex]]
		+	[[Category: Metal ion binding]]
		+	[[Category: Nucleotide binding]]
		+	[[Category: Ribokinase activity]]
		+	[[Category: Transferase]]

OCA at 08:19, 21 April 2021

OCA — Wed, 21 Apr 2021 08:19:34 GMT

←Older revision		Revision as of 08:19, 21 April 2021
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6wb7 is ~~ON HOLD until Paper Publication~~	+	==Acarbose Kinase AcbK as a Complex with Acarbose and AMP-PNP==
-		+	<StructureSection load='6wb7' size='340' side='right'caption='[[6wb7]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WB7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WB7 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wb7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wb7 OCA], [https://pdbe.org/6wb7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wb7 RCSB], [https://www.ebi.ac.uk/pdbsum/6wb7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wb7 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Balaich JN]]
		+	[[Category: Donia MS]]
		+	[[Category: Estrella MA]]
		+	[[Category: Jeffrey PD]]

OCA at 03:59, 13 May 2020

OCA — Wed, 13 May 2020 03:59:44 GMT

←Older revision		Revision as of 03:59, 13 May 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6wb7 is ON HOLD	+	The entry 6wb7 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6wb7" [edit=sysop:move=sysop]

OCA — Wed, 01 Apr 2020 07:36:34 GMT

Protected "6wb7" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:36, 1 April 2020

OCA: New page: '''Unreleased structure''' The entry 6wb7 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 01 Apr 2020 07:36:33 GMT

New page: '''Unreleased structure''' The entry 6wb7 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6wb7 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]