OCA at 14:20, 18 October 2023

OCA — Wed, 18 Oct 2023 14:20:43 GMT

←Older revision		Revision as of 14:20, 18 October 2023
Line 1:		Line 1:

	==High resolution crystal structure of mRECK(CC4) in fusion with engineered MBP==		==High resolution crystal structure of mRECK(CC4) in fusion with engineered MBP==
-	<StructureSection load='6wbj' size='340' side='right'caption='[[6wbj]]' scene=''>	+	<StructureSection load='6wbj' size='340' side='right'caption='[[6wbj]], [[Resolution\|resolution]] 1.65Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WBJ OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6WBJ FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wbj]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli] and [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WBJ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WBJ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6wbj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wbj OCA], [~~http~~://pdbe.org/6wbj PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6wbj RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6wbj PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6wbj ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.651Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=PRD_900001:alpha-maltose'>PRD_900001</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wbj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wbj OCA], [https://pdbe.org/6wbj PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wbj RCSB], [https://www.ebi.ac.uk/pdbsum/6wbj PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wbj ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/MALE_ECOLI MALE_ECOLI] Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.[https://www.uniprot.org/uniprot/RECK_MOUSE RECK_MOUSE] Functions together with ADGRA2 to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed:28803732). Plays a key role in Wnt7-specific responses: required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed:26658478, PubMed:28803732). Acts as a Wnt7-specific coactivator of canonical Wnt signaling by decoding Wnt ligands: acts by interacting specifically with the disordered linker region of Wnt7, thereby conferring ligand selectivity for Wnt7 (By similarity). ADGRA2 is then required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (By similarity). Also acts as a serine protease inhibitor: negatively regulates matrix metalloproteinase-9 (MMP9) by suppressing MMP9 secretion and by direct inhibition of its enzymatic activity (PubMed:11747814). Also inhibits metalloproteinase activity of MMP2 and MMP14 (MT1-MMP) (PubMed:11747814).[UniProtKB:O95980]<ref>PMID:11747814</ref> <ref>PMID:26658478</ref> <ref>PMID:28803732</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Five small protein domains, the CC-domains, at the N terminus of the RECK protein, play essential roles in signaling by WNT7A and WNT7B in the context of central nervous system angiogenesis and blood-brain barrier formation and maintenance. We have determined the structure of CC domain 4 (CC4) at 1.65-A resolution and find that it folds into a compact four-helix bundle with three disulfide bonds. The CC4 structure, together with homology modeling of CC1, reveals the surface locations of critical residues that were shown in previous mutagenesis studies to mediate GPR124 binding and WNT7A/WNT7B recognition and signaling. Surprisingly, sequence and structural homology searches reveal no other cell-surface or secreted domains in vertebrates that resemble the CC domain, a pattern that is in striking contrast to other ancient and similarly sized domains, such as Epidermal Growth Factor, Fibronectin Type 3, Immunoglobulin, and Thrombospondin type 1 domains, which are collectively present in hundreds of proteins.
		+
		+	Structure of the RECK CC domain, an evolutionary anomaly.,Chang TH, Hsieh FL, Smallwood PM, Gabelli SB, Nathans J Proc Natl Acad Sci U S A. 2020 Jun 15. pii: 2006332117. doi:, 10.1073/pnas.2006332117. PMID:32541044<ref>PMID:32541044</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6wbj" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Maltose-binding protein 3D structures\|Maltose-binding protein 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Mus musculus]]
	[[Category: Chang TH]]		[[Category: Chang TH]]
	[[Category: Gabelli SB]]		[[Category: Gabelli SB]]
	[[Category: Hsieh FL]]		[[Category: Hsieh FL]]
	[[Category: Nathans J]]		[[Category: Nathans J]]

OCA at 10:15, 17 June 2020

OCA — Wed, 17 Jun 2020 10:15:19 GMT

←Older revision		Revision as of 10:15, 17 June 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6wbj is ~~ON HOLD until Paper Publication~~	+	==High resolution crystal structure of mRECK(CC4) in fusion with engineered MBP==
-		+	<StructureSection load='6wbj' size='340' side='right'caption='[[6wbj]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WBJ OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WBJ FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wbj FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wbj OCA], [http://pdbe.org/6wbj PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wbj RCSB], [http://www.ebi.ac.uk/pdbsum/6wbj PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wbj ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Chang TH]]
		+	[[Category: Gabelli SB]]
		+	[[Category: Hsieh FL]]
		+	[[Category: Nathans J]]

OCA at 05:10, 29 April 2020

OCA — Wed, 29 Apr 2020 05:10:34 GMT

←Older revision		Revision as of 05:10, 29 April 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6wbj is ON HOLD	+	The entry 6wbj is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6wbj" [edit=sysop:move=sysop]

OCA — Wed, 01 Apr 2020 07:37:42 GMT

Protected "6wbj" [edit=sysop:move=sysop]

←Older revision

Revision as of 07:37, 1 April 2020

OCA: New page: '''Unreleased structure''' The entry 6wbj is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 01 Apr 2020 07:37:39 GMT

New page: '''Unreleased structure''' The entry 6wbj is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6wbj is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6wbj - Revision history

OCA at 14:20, 18 October 2023

OCA at 10:15, 17 June 2020

OCA at 05:10, 29 April 2020

OCA: Protected "6wbj" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6wbj is ON HOLD Authors: Description: Category: Unreleased Structures