6wf1 - Revision history

OCA at 14:23, 18 October 2023

OCA — Wed, 18 Oct 2023 14:23:56 GMT

←Older revision		Revision as of 14:23, 18 October 2023
Line 1:		Line 1:

-	====	+	==Crystal Structure of Broadly Neutralizing Antibody 3I14 Bound to the Influenza A H10 Hemagglutinin==
-	<StructureSection load='6wf1' size='340' side='right'caption='[[6wf1]]' scene=''>	+	<StructureSection load='6wf1' size='340' side='right'caption='[[6wf1]], [[Resolution\|resolution]] 4.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wf1]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Influenza_A_virus Influenza A virus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WF1 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WF1 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wf1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wf1 OCA], [https://pdbe.org/6wf1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wf1 RCSB], [https://www.ebi.ac.uk/pdbsum/6wf1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wf1 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 4.19Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wf1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wf1 OCA], [https://pdbe.org/6wf1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wf1 RCSB], [https://www.ebi.ac.uk/pdbsum/6wf1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wf1 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/A0A059T4A1_9INFA A0A059T4A1_9INFA] Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization of about two third of the virus particles through clathrin-dependent endocytosis and about one third through a clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.[RuleBase:RU003324][SAAS:SAAS00046810]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Broadly neutralizing antibodies (bnAbs) targeting conserved influenza A virus (IAV) hemagglutinin (HA) epitopes can provide valuable information for accelerating universal vaccine designs. Here, we report structural details for heterosubtypic recognition of HA from circulating and emerging IAVs by the human antibody 3I14. Somatic hypermutations play a critical role in shaping the HCDR3, which alone and uniquely among V(H)3-30 derived antibodies, forms contacts with five sub-pockets within the HA-stem hydrophobic groove. 3I14 light-chain interactions are also key for binding HA and contribute a large buried surface area spanning two HA protomers. Comparison of 3I14 to bnAbs from several defined classes provide insights to the bias selection of V(H)3-30 antibodies and reveals that 3I14 represents a novel structural solution within the V(H)3-30 repertoire. The structures reported here improve our understanding of cross-group heterosubtypic binding activity, providing the basis for advancing immunogen designs aimed at eliciting a broadly protective response to IAV.
		+
		+	Unique structural solution from a V(H)3-30 antibody targeting the hemagglutinin stem of influenza A viruses.,Harshbarger WD, Deming D, Lockbaum GJ, Attatippaholkun N, Kamkaew M, Hou S, Somasundaran M, Wang JP, Finberg RW, Zhu QK, Schiffer CA, Marasco WA Nat Commun. 2021 Jan 25;12(1):559. doi: 10.1038/s41467-020-20879-6. PMID:33495478<ref>PMID:33495478</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6wf1" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[Hemagglutinin 3D structures\|Hemagglutinin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Influenza A virus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Attatippaholkun N]]
		+	[[Category: Deming DT]]
		+	[[Category: Harshbarger WD]]
		+	[[Category: Lockbaum GJ]]
		+	[[Category: Marasco WA]]
		+	[[Category: Schiffer CA]]

OCA at 12:25, 9 June 2021

OCA — Wed, 09 Jun 2021 12:25:10 GMT

←Older revision		Revision as of 12:25, 9 June 2021
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	<StructureSection load='6wf1' size='340' side='right'caption='[[6wf1]]' scene=''>		<StructureSection load='6wf1' size='340' side='right'caption='[[6wf1]]' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6wf1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wf1 OCA], [~~http~~://pdbe.org/6wf1 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6wf1 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6wf1 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6wf1 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wf1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wf1 OCA], [https://pdbe.org/6wf1 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wf1 RCSB], [https://www.ebi.ac.uk/pdbsum/6wf1 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wf1 ProSAT]</span></td></tr>
	</table>		</table>
	__TOC__		__TOC__

OCA at 06:54, 25 November 2020

OCA — Wed, 25 Nov 2020 06:54:41 GMT

←Older revision		Revision as of 06:54, 25 November 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6wf1 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6wf1' size='340' side='right'caption='[[6wf1]]' scene=''>
-	~~Authors~~: ~~Harshbarger, W~~.D.~~, Lockbaum, G~~.J.~~, Deming, D~~.T.~~, Attatippaholkun, N~~.~~, Schiffer, C~~.A., ~~Marasco, W~~.A.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description: Crystal Structure of Broadly Neutralizing Antibody 3I14 Bound to the Influenza A H10 Hemagglutinin~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wf1 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wf1 OCA], [http://pdbe.org/6wf1 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wf1 RCSB], [http://www.ebi.ac.uk/pdbsum/6wf1 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wf1 ProSAT]</span></td></tr>
-	[~~[Category~~: ~~Unreleased Structures~~]]	+	</table>
-	~~[[Category: Attatippaholkun~~, ~~N]]~~	+	__TOC__
-	[~~[Category~~: ~~Deming, D~~.T]]	+	</StructureSection>
-	[~~[Category~~: ~~Harshbarger, W~~.D]]	+	[[Category: Large Structures]]
-	[~~[Category~~: ~~Lockbaum, G~~.J]]	+	[[Category: Z-disk]]
-	[[Category: ~~Schiffer, C.A~~]]	+
-	[[Category: ~~Marasco, W.A~~]]	+

OCA at 07:46, 18 November 2020

OCA — Wed, 18 Nov 2020 07:46:53 GMT

←Older revision		Revision as of 07:46, 18 November 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6wf1 is ON HOLD	+	The entry 6wf1 is ON HOLD until Paper Publication

	Authors: Harshbarger, W.D., Lockbaum, G.J., Deming, D.T., Attatippaholkun, N., Schiffer, C.A., Marasco, W.A.		Authors: Harshbarger, W.D., Lockbaum, G.J., Deming, D.T., Attatippaholkun, N., Schiffer, C.A., Marasco, W.A.

OCA: Protected "6wf1" [edit=sysop:move=sysop]

OCA — Wed, 15 Apr 2020 05:22:07 GMT

Protected "6wf1" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:22, 15 April 2020

OCA: New page: '''Unreleased structure''' The entry 6wf1 is ON HOLD Authors: Harshbarger, W.D., Lockbaum, G.J., Deming, D.T., Attatippaholkun, N., Schiffer, C.A., Marasco, W.A. Description: Crystal S...

OCA — Wed, 15 Apr 2020 05:22:06 GMT

New page: '''Unreleased structure''' The entry 6wf1 is ON HOLD Authors: Harshbarger, W.D., Lockbaum, G.J., Deming, D.T., Attatippaholkun, N., Schiffer, C.A., Marasco, W.A. Description: Crystal S...

New page

'''Unreleased structure'''

The entry 6wf1 is ON HOLD

Authors: Harshbarger, W.D., Lockbaum, G.J., Deming, D.T., Attatippaholkun, N., Schiffer, C.A., Marasco, W.A.

Description: Crystal Structure of Broadly Neutralizing Antibody 3I14 Bound to the Influenza A H10 Hemagglutinin
[[Category: Unreleased Structures]]
[[Category: Attatippaholkun, N]]
[[Category: Deming, D.T]]
[[Category: Harshbarger, W.D]]
[[Category: Lockbaum, G.J]]
[[Category: Schiffer, C.A]]
[[Category: Marasco, W.A]]