OCA at 14:24, 18 October 2023

OCA — Wed, 18 Oct 2023 14:24:07 GMT

←Older revision		Revision as of 14:24, 18 October 2023
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6wf5 is ~~ON HOLD until Paper~~ Publication	+	==Crystal structure of human Naa50 in complex with a truncated cofactor derived inhibitor (compound 2)==
		+	<StructureSection load='6wf5' size='340' side='right'caption='[[6wf5]], [[Resolution\|resolution]] 2.04Å' scene=''>
		+	== Structural highlights ==
		+	<table><tr><td colspan='2'>[[6wf5]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens] and [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WF5 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WF5 FirstGlance]. <br>
		+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.04Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACE:ACETYL+GROUP'>ACE</scene>, <scene name='pdbligand=NH2:AMINO+GROUP'>NH2</scene>, <scene name='pdbligand=U44:(2R)-2-hydroxy-3,3-dimethyl-N-{3-oxo-3-[(2-sulfanylethyl)amino]propyl}butanamide'>U44</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wf5 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wf5 OCA], [https://pdbe.org/6wf5 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wf5 RCSB], [https://www.ebi.ac.uk/pdbsum/6wf5 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wf5 ProSAT]</span></td></tr>
		+	</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/NAA50_HUMAN NAA50_HUMAN] Probable catalytic component of the NAA11-NAA15 complex which displays alpha (N-terminal) acetyltransferase activity.<ref>PMID:16507339</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Two novel compounds were identified as Naa50 binders/inhibitors using DNA-encoded technology screening. Biophysical and biochemical data as well as cocrystal structures were obtained for both compounds (3a and 4a) to understand their mechanism of action. These data were also used to rationalize the binding affinity differences observed between the two compounds and a MLGP peptide-containing substrate. Cellular target engagement experiments further confirm the Naa50 binding of 4a and demonstrate its selectivity toward related enzymes (Naa10 and Naa60). Additional analogs of inhibitor 4a were also evaluated to study the binding mode observed in the cocrystal structures.

-	~~Authors:~~ Greasley, S~~.E.~~, ~~Feng~~, J., ~~Deng~~, Y.-L., ~~Stewart, A~~.E.	+	Characterization of Specific N-alpha-Acetyltransferase 50 (Naa50) Inhibitors Identified Using a DNA Encoded Library.,Kung PP, Bingham P, Burke BJ, Chen Q, Cheng X, Deng YL, Dou D, Feng J, Gallego GM, Gehring MR, Grant SK, Greasley S, Harris AR, Maegley KA, Meier J, Meng X, Montano JL, Morgan BA, Naughton BS, Palde PB, Paul TA, Richardson P, Sakata S, Shaginian A, Sonnenburg WK, Subramanyam C, Timofeevski S, Wan J, Yan W, Stewart AE ACS Med Chem Lett. 2020 Apr 10;11(6):1175-1184. doi:, 10.1021/acsmedchemlett.0c00029. eCollection 2020 Jun 11. PMID:32550998<ref>PMID:32550998</ref>

-	~~Description: Crystal structure of human Naa50 in complex with~~ a ~~truncated cofactor derived inhibitor (compound 2)~~	+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	[[Category: ~~Unreleased Structures~~]]	+	</div>
-	[[Category: ~~Feng, J~~]]	+	<div class="pdbe-citations 6wf5" style="background-color:#fffaf0;"></div>
-	[[Category: ~~Greasley, S.E~~]]	+	== References ==
-	[[Category: Deng, Y.-L]]	+	<references/>
-	[[Category: Stewart~~, A.E~~]]	+	__TOC__
		+	</StructureSection>
		+	[[Category: Homo sapiens]]
		+	[[Category: Large Structures]]
		+	[[Category: Synthetic construct]]
		+	[[Category: Deng Y-L]]
		+	[[Category: Feng J]]
		+	[[Category: Greasley SE]]
		+	[[Category: Stewart AE]]

OCA at 04:11, 20 May 2020

OCA — Wed, 20 May 2020 04:11:40 GMT

←Older revision		Revision as of 04:11, 20 May 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6wf5 is ON HOLD	+	The entry 6wf5 is ON HOLD until Paper Publication

	Authors: Greasley, S.E., Feng, J., Deng, Y.-L., Stewart, A.E.		Authors: Greasley, S.E., Feng, J., Deng, Y.-L., Stewart, A.E.

OCA: Protected "6wf5" [edit=sysop:move=sysop]

OCA — Wed, 15 Apr 2020 05:22:41 GMT

Protected "6wf5" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:22, 15 April 2020

OCA: New page: '''Unreleased structure''' The entry 6wf5 is ON HOLD Authors: Greasley, S.E., Feng, J., Deng, Y.-L., Stewart, A.E. Description: Crystal structure of human Naa50 in complex with a trunc...

OCA — Wed, 15 Apr 2020 05:22:40 GMT

New page: '''Unreleased structure''' The entry 6wf5 is ON HOLD Authors: Greasley, S.E., Feng, J., Deng, Y.-L., Stewart, A.E. Description: Crystal structure of human Naa50 in complex with a trunc...

New page

'''Unreleased structure'''

The entry 6wf5 is ON HOLD

Authors: Greasley, S.E., Feng, J., Deng, Y.-L., Stewart, A.E.

Description: Crystal structure of human Naa50 in complex with a truncated cofactor derived inhibitor (compound 2)
[[Category: Unreleased Structures]]
[[Category: Feng, J]]
[[Category: Greasley, S.E]]
[[Category: Deng, Y.-L]]
[[Category: Stewart, A.E]]

6wf5 - Revision history

OCA at 14:24, 18 October 2023

OCA at 04:11, 20 May 2020

OCA: Protected "6wf5" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6wf5 is ON HOLD Authors: Greasley, S.E., Feng, J., Deng, Y.-L., Stewart, A.E. Description: Crystal structure of human Naa50 in complex with a trunc...