6whi - Revision history

OCA at 14:44, 6 March 2024

OCA — Wed, 06 Mar 2024 14:44:20 GMT

←Older revision		Revision as of 14:44, 6 March 2024
Line 3:		Line 3:
	<StructureSection load='6whi' size='340' side='right'caption='[[6whi]], [[Resolution\|resolution]] 4.20Å' scene=''>		<StructureSection load='6whi' size='340' side='right'caption='[[6whi]], [[Resolution\|resolution]] 4.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6whi]] is a 16 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/ ~~], [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885~~] and [~~http~~://en.wikipedia.org/wiki/~~Atcc_33519 Atcc 33519~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WHI OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6WHI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6whi]] is a 16 chain structure with sequence from [https://en.wikipedia.org/wiki/Proteus_penneri Proteus penneri] and [https://en.wikipedia.org/wiki/Pseudomonas_aeruginosa Pseudomonas aeruginosa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WHI OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WHI FirstGlance]. <br>
-	</td></tr><tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat"~~>csy1, PA14_33330 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&~~id=~~287~~ "~~Bacillus aeruginosus~~" ~~(Schroeter 1872) Trevisan 1885])~~, ~~csy2, ALP65_00953, EQH76_13810, FCG96_17775, PACL_0128 (~~[~~http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885]), EQH76_13805, FCG96_17770 (~~[~~http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885~~]~~), cas6f, csy4, PA14_33300 ([http://www~~.~~ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info~~&~~srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 4.2Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6whi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6whi OCA], [~~http~~://pdbe.org/6whi PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6whi RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6whi PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6whi ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6whi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6whi OCA], [https://pdbe.org/6whi PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6whi RCSB], [https://www.ebi.ac.uk/pdbsum/6whi PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6whi ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/CSY1_PSEAB CSY1_PSEAB]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 and Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). Involved in crRNA production or stability. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:21398535</ref> <ref>PMID:22522703</ref> [[http://www.uniprot.org/uniprot/CAS6_PSEAB CAS6_PSEAB]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Processes pre-crRNA into individual crRNA units. Absolutely required for crRNA production or stability. Upon expression in E.coli endonucleolytically processes pre-crRNA, although disruption and reconstitution experiments indicate that in situ other genes are also required for processing. Yields 5'-hydroxy and 3'-phosphate groups. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:20829488</ref> <ref>PMID:21398535</ref> <ref>PMID:22522703</ref>	+	[https://www.uniprot.org/uniprot/CSY1_PSEAB CSY1_PSEAB] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 and Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). Involved in crRNA production or stability. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:21398535</ref> <ref>PMID:22522703</ref>
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Bacteria have evolved sophisticated adaptive immune systems, called CRISPR-Cas, that provide sequence-specific protection against phage infection. In turn, phages have evolved a broad spectrum of anti-CRISPRs that suppress these immune systems. Here we report structures of anti-CRISPR protein IF9 (AcrIF9) in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy). In addition to sterically blocking the hybridization of complementary dsDNA to the CRISPR RNA, our results show that AcrIF9 binding also promotes non-sequence-specific engagement with dsDNA, potentially sequestering the complex from target DNA. These findings highlight the versatility of anti-CRISPR mechanisms utilized by phages to suppress CRISPR-mediated immune systems.	+

-	AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex.,Hirschi M, Lu WT, Santiago-Frangos A, Wilkinson R, Golden SM, Davidson AR, Lander GC, Wiedenheft B Nat Commun. 2020 Jun 1;11(1):2730. doi: 10.1038/s41467-020-16512-1. PMID:32483187<ref>PMID:32483187</ref>	+	==See Also==
-		+	*[[Endonuclease 3D structures\|Endonuclease 3D structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class~~=~~"pdbe-citations 6whi" style~~=~~"background-color:#fffaf0;"></div>~~	+
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: Atcc 33519]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Golden, S M~~]]	+	[[Category: Proteus penneri]]
-	[[Category: ~~Hirschi, M~~]]	+	[[Category: Pseudomonas aeruginosa]]
-	[[Category: ~~Lander, G~~]]	+	[[Category: Golden SM]]
-	[[Category: ~~Santiago-Frangos, A~~]]	+	[[Category: Hirschi M]]
-	[[Category: ~~Wiedenheft, B~~]]	+	[[Category: Lander G]]
-	[[Category: ~~Wilkinson, R~~]]	+	[[Category: Santiago-Frangos A]]
-	[[Category: ~~Csy complex~~]]	+	[[Category: Wiedenheft B]]
-	[[Category: ~~Immune system-dna-rna complex]]~~	+	[[Category: Wilkinson R]]
-	~~[[Category: Type i-f crispr rna-guided surveillance complex~~]]	+

OCA at 10:49, 17 June 2020

OCA — Wed, 17 Jun 2020 10:49:55 GMT

←Older revision		Revision as of 10:49, 17 June 2020
Line 1:		Line 1:

	==Cryo-electron microscopy structure of the type I-F CRISPR RNA-guided surveillance complex bound to the anti-CRISPR AcrIF9==		==Cryo-electron microscopy structure of the type I-F CRISPR RNA-guided surveillance complex bound to the anti-CRISPR AcrIF9==
-	<StructureSection load='6whi' size='340' side='right'caption='[[6whi]]' scene=''>	+	<StructureSection load='6whi' size='340' side='right'caption='[[6whi]], [[Resolution\|resolution]] 4.20Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WHI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WHI FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6whi]] is a 16 chain structure with sequence from [http://en.wikipedia.org/wiki/ ], [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885] and [http://en.wikipedia.org/wiki/Atcc_33519 Atcc 33519]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WHI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WHI FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6whi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6whi OCA], [http://pdbe.org/6whi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6whi RCSB], [http://www.ebi.ac.uk/pdbsum/6whi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6whi ProSAT]</span></td></tr>	+	</td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">csy1, PA14_33330 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885]), csy2, ALP65_00953, EQH76_13810, FCG96_17775, PACL_0128 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885]), EQH76_13805, FCG96_17770 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885]), cas6f, csy4, PA14_33300 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6whi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6whi OCA], [http://pdbe.org/6whi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6whi RCSB], [http://www.ebi.ac.uk/pdbsum/6whi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6whi ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/CSY1_PSEAB CSY1_PSEAB]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Cas3 and Cascade participate in CRISPR interference, the third stage of CRISPR immunity (Potential). Involved in crRNA production or stability. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:21398535</ref> <ref>PMID:22522703</ref> [[http://www.uniprot.org/uniprot/CAS6_PSEAB CAS6_PSEAB]] CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain sequences complementary to antecedent mobile elements and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Processes pre-crRNA into individual crRNA units. Absolutely required for crRNA production or stability. Upon expression in E.coli endonucleolytically processes pre-crRNA, although disruption and reconstitution experiments indicate that in situ other genes are also required for processing. Yields 5'-hydroxy and 3'-phosphate groups. The Csy ribonucleoprotein complex binds target ssDNA with high affinity but target dsDNA with much lower affinity.<ref>PMID:20829488</ref> <ref>PMID:21398535</ref> <ref>PMID:22522703</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Bacteria have evolved sophisticated adaptive immune systems, called CRISPR-Cas, that provide sequence-specific protection against phage infection. In turn, phages have evolved a broad spectrum of anti-CRISPRs that suppress these immune systems. Here we report structures of anti-CRISPR protein IF9 (AcrIF9) in complex with the type I-F CRISPR RNA-guided surveillance complex (Csy). In addition to sterically blocking the hybridization of complementary dsDNA to the CRISPR RNA, our results show that AcrIF9 binding also promotes non-sequence-specific engagement with dsDNA, potentially sequestering the complex from target DNA. These findings highlight the versatility of anti-CRISPR mechanisms utilized by phages to suppress CRISPR-mediated immune systems.
		+
		+	AcrIF9 tethers non-sequence specific dsDNA to the CRISPR RNA-guided surveillance complex.,Hirschi M, Lu WT, Santiago-Frangos A, Wilkinson R, Golden SM, Davidson AR, Lander GC, Wiedenheft B Nat Commun. 2020 Jun 1;11(1):2730. doi: 10.1038/s41467-020-16512-1. PMID:32483187<ref>PMID:32483187</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6whi" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Atcc 33519]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Golden SM]]	+	[[Category: Golden, S M]]
-	[[Category: Hirschi M]]	+	[[Category: Hirschi, M]]
-	[[Category: Lander G]]	+	[[Category: Lander, G]]
-	[[Category: Santiago-Frangos A]]	+	[[Category: Santiago-Frangos, A]]
-	[[Category: Wiedenheft B]]	+	[[Category: Wiedenheft, B]]
-	[[Category: Wilkinson R]]	+	[[Category: Wilkinson, R]]
		+	[[Category: Csy complex]]
		+	[[Category: Immune system-dna-rna complex]]
		+	[[Category: Type i-f crispr rna-guided surveillance complex]]

OCA at 05:36, 13 May 2020

OCA — Wed, 13 May 2020 05:36:51 GMT

←Older revision		Revision as of 05:36, 13 May 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6whi is ~~ON HOLD until sometime in~~ the ~~future~~	+	==Cryo-electron microscopy structure of the type I-F CRISPR RNA-guided surveillance complex bound to the anti-CRISPR AcrIF9==
-		+	<StructureSection load='6whi' size='340' side='right'caption='[[6whi]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WHI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WHI FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6whi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6whi OCA], [http://pdbe.org/6whi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6whi RCSB], [http://www.ebi.ac.uk/pdbsum/6whi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6whi ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Golden SM]]
		+	[[Category: Hirschi M]]
		+	[[Category: Lander G]]
		+	[[Category: Santiago-Frangos A]]
		+	[[Category: Wiedenheft B]]
		+	[[Category: Wilkinson R]]

OCA: Protected "6whi" [edit=sysop:move=sysop]

OCA — Wed, 15 Apr 2020 05:28:10 GMT

Protected "6whi" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:28, 15 April 2020

OCA: New page: '''Unreleased structure''' The entry 6whi is ON HOLD until sometime in the future Authors: Description: Category: Unreleased Structures

OCA — Wed, 15 Apr 2020 05:28:09 GMT

New page: '''Unreleased structure''' The entry 6whi is ON HOLD until sometime in the future Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6whi is ON HOLD until sometime in the future

Authors:

Description:
[[Category: Unreleased Structures]]