OCA at 14:40, 8 July 2020

OCA — Wed, 08 Jul 2020 14:40:46 GMT

←Older revision		Revision as of 14:40, 8 July 2020
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	==Copper resistance protein copG- Form 1==		==Copper resistance protein copG- Form 1==
-	<StructureSection load='6wis' size='340' side='right'caption='[[6wis]]' scene=''>	+	<StructureSection load='6wis' size='340' side='right'caption='[[6wis]], [[Resolution\|resolution]] 2.00Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WIS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WIS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wis]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_aeruginosus"_(schroeter_1872)_trevisan_1885 "bacillus aeruginosus" (schroeter 1872) trevisan 1885]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WIS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WIS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wis OCA], [http://pdbe.org/6wis PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wis RCSB], [http://www.ebi.ac.uk/pdbsum/6wis PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wis ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CU:COPPER+(II)+ION'>CU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene></td></tr>
		+	<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue\|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">FQ758_25220 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=287 "Bacillus aeruginosus" (Schroeter 1872) Trevisan 1885])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wis OCA], [http://pdbe.org/6wis PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wis RCSB], [http://www.ebi.ac.uk/pdbsum/6wis PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wis ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	CopG is an uncharacterized protein ubiquitous in gram-negative bacteria whose gene frequently occurs in clusters of copper-resistance genes and can be recognized by the presence of a conserved CxCC motif. To investigate its contribution to copper resistance, here we undertook a structural and biochemical characterization of the CopG protein from Pseudomonas aeruginosaResults from biochemical analyses of CopG purified under aerobic conditions indicate that it is a green copper-binding protein that displays absorbance maxima near 411, 581, and 721 nm and is monomeric in solution. Determination of the three-dimensional structure by X-ray crystallography revealed that CopG consists of a thioredoxin domain with a C-terminal extension that contributes to metal binding. We noted that adjacent to the CxCC motif is a cluster of four copper ions bridged by cysteine sulfur atoms. Structures of CopG in two oxidation states support the assignment of this protein as an oxidoreductase. On the basis of these structural and spectroscopic findings and also genetic evidence, we propose that CopG has a role in interconverting Cu(I) and Cu(II) to minimize toxic effects and facilitate export by the Cus RND transporter efflux system.
		+
		+	The bacterial copper-resistance protein CopG contains a cysteine-bridged tetranuclear copper cluster.,Hausrath AC, Ramirez NA, Ly AT, McEvoy MM J Biol Chem. 2020 Jun 22. pii: RA120.013907. doi: 10.1074/jbc.RA120.013907. PMID:32571874<ref>PMID:32571874</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6wis" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Hausrath AC]]	+	[[Category: Hausrath, A C]]
-	[[Category: Ly A]]	+	[[Category: Ly, A]]
-	[[Category: McEvoy MM]]	+	[[Category: McEvoy, M M]]
		+	[[Category: Metal binding protein]]
		+	[[Category: Metal resistance copper-binding protein]]

OCA at 06:27, 25 June 2020

OCA — Thu, 25 Jun 2020 06:27:59 GMT

←Older revision		Revision as of 06:27, 25 June 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6wis is ~~ON HOLD until Paper Publication~~	+	==Copper resistance protein copG- Form 1==
-		+	<StructureSection load='6wis' size='340' side='right'caption='[[6wis]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WIS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WIS FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wis FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wis OCA], [http://pdbe.org/6wis PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wis RCSB], [http://www.ebi.ac.uk/pdbsum/6wis PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wis ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Hausrath AC]]
		+	[[Category: Ly A]]
		+	[[Category: McEvoy MM]]

OCA: Protected "6wis" [edit=sysop:move=sysop]

OCA — Wed, 22 Apr 2020 04:15:42 GMT

Protected "6wis" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:15, 22 April 2020

OCA: New page: '''Unreleased structure''' The entry 6wis is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

OCA — Wed, 22 Apr 2020 04:15:41 GMT

New page: '''Unreleased structure''' The entry 6wis is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6wis is ON HOLD until Paper Publication

Authors:

Description:
[[Category: Unreleased Structures]]

6wis - Revision history

OCA at 14:40, 8 July 2020

OCA at 06:27, 25 June 2020

OCA: Protected "6wis" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6wis is ON HOLD until Paper Publication Authors: Description: Category: Unreleased Structures