6wjc - Revision history

OCA at 14:26, 18 October 2023

OCA — Wed, 18 Oct 2023 14:26:58 GMT

←Older revision		Revision as of 14:26, 18 October 2023
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	<StructureSection load='6wjc' size='340' side='right'caption='[[6wjc]], [[Resolution\|resolution]] 2.55Å' scene=''>		<StructureSection load='6wjc' size='340' side='right'caption='[[6wjc]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6wjc]] is a 2 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Bmnh_1946~~.1.~~20.48 Bmnh 1946.1.20.48~~] and [~~http~~://en.wikipedia.org/wiki/~~Human Human~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WJC OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6WJC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wjc]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Dendroaspis_angusticeps Dendroaspis angusticeps], [https://en.wikipedia.org/wiki/Escherichia_virus_T4 Escherichia virus T4] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WJC OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WJC FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACM:ACETAMIDE'>ACM</scene>, <scene name='pdbligand=OIN:(1R,5S)-8-METHYL-8-AZABICYCLO[3.2.1]OCT-3-YL+(2R)-3-HYDROXY-2-PHENYLPROPANOATE'>OIN</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene~~></td></tr>~~	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.55Å</td></tr>
-	~~<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CHRM1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>~~	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACM:ACETAMIDE'>ACM</scene>, <scene name='pdbligand=OIN:(1R,5S)-8-METHYL-8-AZABICYCLO[3.2.1]OCT-3-YL+(2R)-3-HYDROXY-2-PHENYLPROPANOATE'>OIN</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
-	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wjc OCA], [https://pdbe.org/6wjc PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wjc RCSB], [https://www.ebi.ac.uk/pdbsum/6wjc PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wjc ProSAT]</span></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6wjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wjc OCA], [~~http~~://pdbe.org/6wjc PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6wjc RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6wjc PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6wjc ProSAT]</span></td></tr>	+
	</table>		</table>
	== Function ==		== Function ==
-	[[~~http~~://www.uniprot.org/uniprot/ACM1_HUMAN ACM1_HUMAN]] The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. [[http://www.uniprot.org/uniprot/3SIM7_DENAN 3SIM7_DENAN]] Binds irreversibly and specifically to an allosteric site of the muscarinic acetylcholine M1 receptor (CHRM1).<ref>PMID:10799315</ref> <ref>PMID:11562434</ref> <ref>PMID:12488533</ref> <ref>PMID:21557730</ref>	+	[https://www.uniprot.org/uniprot/D9IEF7_BPT4 D9IEF7_BPT4] [https://www.uniprot.org/uniprot/ACM1_HUMAN ACM1_HUMAN] The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover.
	<div style="background-color:#fffaf0;">		<div style="background-color:#fffaf0;">
	== Publication Abstract from PubMed ==		== Publication Abstract from PubMed ==
Line 20:		Line 19:
	</div>		</div>
	<div class="pdbe-citations 6wjc" style="background-color:#fffaf0;"></div>		<div class="pdbe-citations 6wjc" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Muscarinic acetylcholine receptor\|Muscarinic acetylcholine receptor]]
	== References ==		== References ==
	<references/>		<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Bmnh 1946 1.20 48~~]]	+	[[Category: Dendroaspis angusticeps]]
-	[[Category: ~~Human~~]]	+	[[Category: Escherichia virus T4]]
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	~~[[Category: Lysozyme]]~~	+	[[Category: Kobilka BK]]
-	[[Category: Kobilka~~, B K~~]]	+	[[Category: Maeda S]]
-	[[Category: Maeda, S]]	+
-	~~[[Category: Gpcr]]~~	+
-	~~[[Category: Membrane protein]]~~	+
-	~~[[Category: Natural toxin~~]]	+

OCA at 11:44, 22 July 2020

OCA — Wed, 22 Jul 2020 11:44:51 GMT

←Older revision		Revision as of 11:44, 22 July 2020
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	==Muscarinic acetylcholine receptor 1 - muscarinic toxin 7 complex==		==Muscarinic acetylcholine receptor 1 - muscarinic toxin 7 complex==
-	<StructureSection load='6wjc' size='340' side='right'caption='[[6wjc]]' scene=''>	+	<StructureSection load='6wjc' size='340' side='right'caption='[[6wjc]], [[Resolution\|resolution]] 2.55Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WJC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WJC FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wjc]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bmnh_1946.1.20.48 Bmnh 1946.1.20.48] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WJC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WJC FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wjc OCA], [http://pdbe.org/6wjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wjc RCSB], [http://www.ebi.ac.uk/pdbsum/6wjc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wjc ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=ACM:ACETAMIDE'>ACM</scene>, <scene name='pdbligand=OIN:(1R,5S)-8-METHYL-8-AZABICYCLO[3.2.1]OCT-3-YL+(2R)-3-HYDROXY-2-PHENYLPROPANOATE'>OIN</scene>, <scene name='pdbligand=Y01:CHOLESTEROL+HEMISUCCINATE'>Y01</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">CHRM1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
		+	<tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Lysozyme Lysozyme], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.17 3.2.1.17] </span></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wjc OCA], [http://pdbe.org/6wjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wjc RCSB], [http://www.ebi.ac.uk/pdbsum/6wjc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wjc ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/ACM1_HUMAN ACM1_HUMAN]] The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is Pi turnover. [[http://www.uniprot.org/uniprot/3SIM7_DENAN 3SIM7_DENAN]] Binds irreversibly and specifically to an allosteric site of the muscarinic acetylcholine M1 receptor (CHRM1).<ref>PMID:10799315</ref> <ref>PMID:11562434</ref> <ref>PMID:12488533</ref> <ref>PMID:21557730</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Muscarinic toxins (MTs) are natural toxins produced by mamba snakes that primarily bind to muscarinic acetylcholine receptors (MAChRs) and modulate their function. Despite their similar primary and tertiary structures, MTs show distinct binding selectivity toward different MAChRs. The molecular details of how MTs distinguish MAChRs are not well understood. Here, we present the crystal structure of M1AChR in complex with MT7, a subtype-selective anti-M1AChR snake venom toxin. The structure reveals the molecular basis of the extreme subtype specificity of MT7 for M1AChR and the mechanism by which it regulates receptor function. Through in vitro engineering of MT7 finger regions that was guided by the structure, we have converted the selectivity from M1AChR toward M2AChR, suggesting that the three-finger fold is a promising scaffold for developing G protein-coupled receptor modulators.
		+
		+	Structure and selectivity engineering of the M1 muscarinic receptor toxin complex.,Maeda S, Xu J, N Kadji FM, Clark MJ, Zhao J, Tsutsumi N, Aoki J, Sunahara RK, Inoue A, Garcia KC, Kobilka BK Science. 2020 Jul 10;369(6500):161-167. doi: 10.1126/science.aax2517. PMID:32646996<ref>PMID:32646996</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6wjc" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bmnh 1946 1.20 48]]
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Kobilka BK]]	+	[[Category: Lysozyme]]
-	[[Category: Maeda S]]	+	[[Category: Kobilka, B K]]
		+	[[Category: Maeda, S]]
		+	[[Category: Gpcr]]
		+	[[Category: Membrane protein]]
		+	[[Category: Natural toxin]]

OCA at 14:09, 8 July 2020

OCA — Wed, 08 Jul 2020 14:09:10 GMT

←Older revision		Revision as of 14:09, 8 July 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6wjc is ~~ON HOLD until sometime in~~ the ~~future~~	+	==Muscarinic acetylcholine receptor 1 - muscarinic toxin 7 complex==
-		+	<StructureSection load='6wjc' size='340' side='right'caption='[[6wjc]]' scene=''>
-	~~Authors~~: ~~Maeda~~, S., ~~Kobilka~~, B.K.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WJC OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WJC FirstGlance]. <br>
-	~~Description~~: ~~muscarinic acetylcholine receptor 1~~ -~~muscarinic toxin 7 complex~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wjc FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wjc OCA], [http://pdbe.org/6wjc PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wjc RCSB], [http://www.ebi.ac.uk/pdbsum/6wjc PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wjc ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Maeda, S~~]]	+	__TOC__
-	[[Category: ~~Kobilka, B.K~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Kobilka BK]]
		+	[[Category: Maeda S]]

OCA at 08:32, 17 June 2020

OCA — Wed, 17 Jun 2020 08:32:04 GMT

←Older revision		Revision as of 08:32, 17 June 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6wjc is ON HOLD	+	The entry 6wjc is ON HOLD until sometime in the future

	Authors: Maeda, S., Kobilka, B.K.		Authors: Maeda, S., Kobilka, B.K.

OCA at 04:50, 10 June 2020

OCA — Wed, 10 Jun 2020 04:50:25 GMT

←Older revision		Revision as of 04:50, 10 June 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6wjc is ON HOLD ~~until sometime in the future~~	+	The entry 6wjc is ON HOLD

	Authors: Maeda, S., Kobilka, B.K.		Authors: Maeda, S., Kobilka, B.K.

OCA at 04:03, 13 May 2020

OCA — Wed, 13 May 2020 04:03:03 GMT

←Older revision		Revision as of 04:03, 13 May 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6wjc is ON HOLD	+	The entry 6wjc is ON HOLD until sometime in the future

	Authors: Maeda, S., Kobilka, B.K.		Authors: Maeda, S., Kobilka, B.K.

OCA: Protected "6wjc" [edit=sysop:move=sysop]

OCA — Wed, 22 Apr 2020 04:17:33 GMT

Protected "6wjc" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:17, 22 April 2020

OCA: New page: '''Unreleased structure''' The entry 6wjc is ON HOLD Authors: Maeda, S., Kobilka, B.K. Description: muscarinic acetylcholine receptor 1 -muscarinic toxin 7 complex [[Category: Unreleas...

OCA — Wed, 22 Apr 2020 04:17:31 GMT

New page: '''Unreleased structure''' The entry 6wjc is ON HOLD Authors: Maeda, S., Kobilka, B.K. Description: muscarinic acetylcholine receptor 1 -muscarinic toxin 7 complex [[Category: Unreleas...

New page

'''Unreleased structure'''

The entry 6wjc is ON HOLD

Authors: Maeda, S., Kobilka, B.K.

Description: muscarinic acetylcholine receptor 1 -muscarinic toxin 7 complex
[[Category: Unreleased Structures]]
[[Category: Maeda, S]]
[[Category: Kobilka, B.K]]