OCA at 14:27, 18 October 2023

OCA — Wed, 18 Oct 2023 14:27:31 GMT

←Older revision		Revision as of 14:27, 18 October 2023
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	==Crystal structure of wild-type Arginine Repressor from the pathogenic bacterium Corynebacterium pseudotuberculosis bound to tyrosine==		==Crystal structure of wild-type Arginine Repressor from the pathogenic bacterium Corynebacterium pseudotuberculosis bound to tyrosine==
-	<StructureSection load='6wjo' size='340' side='right'caption='[[6wjo]]' scene=''>	+	<StructureSection load='6wjo' size='340' side='right'caption='[[6wjo]], [[Resolution\|resolution]] 1.69Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WJO OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6WJO FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wjo]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Corynebacterium_pseudotuberculosis_C231 Corynebacterium pseudotuberculosis C231]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WJO OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WJO FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6wjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wjo OCA], [~~http~~://pdbe.org/6wjo PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6wjo RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6wjo PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6wjo ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.693Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NA:SODIUM+ION'>NA</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TYR:TYROSINE'>TYR</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wjo OCA], [https://pdbe.org/6wjo PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wjo RCSB], [https://www.ebi.ac.uk/pdbsum/6wjo PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wjo ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/D9QA55_CORP2 D9QA55_CORP2] Regulates arginine biosynthesis genes.[ARBA:ARBA00002095][HAMAP-Rule:MF_00173]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The arginine repressor (ArgR) regulates the expression of genes involved in arginine biosynthesis. Upon attaining a threshold concentration of arginine in the cytoplasm, the trimeric C-terminal domain of ArgR binds three arginines in a shallow surface cleft and subsequently hexamerizes forming a dimer of trimers containing six Arg co-repressor molecules which are buried at the subunit interfaces. The N-terminal domains of this complex bind to the DNA promoter thereby interrupting the transcription of the genes related to Arg biosynthesis. The crystal structures of the wild type and mutant Pro115Gln ArgR from Corynebacterium pseudotuberculosis determined at 1.7 A demonstrate that a single amino acid substitution switches co-repressor specificity from Tyr to Arg. Molecular dynamics simulations indicate that the first step, i.e., the binding of the co-repressor, occurs in the trimeric state and that Pro115Gln ArgR preferentially binds Arg. It was also shown that, in Pro115 ArgR hexamers, the concomitant binding of sodium ions shifts selectivity to Tyr. Structural data combined with phylogenetic analyses of ArgR from C. pseudotuberculosis suggest that substitutions in the binding pocket at position 115 may alter its specificity for amino acids and that the length of the protein interdomain linker can provide further functional flexibility. These results support the existence of alternative ArgR regulatory mechanisms in this pathogenic bacterium.
		+
		+	A single P115Q mutation modulates specificity in the Corynebacterium pseudotuberculosis arginine repressor.,Mariutti RB, Hernandez-Gonzalez JE, Nascimento AFZ, de Morais MAB, Murakami MT, Carareto CMA, Arni RK Biochim Biophys Acta Gen Subj. 2020 Mar 7;1864(7):129597. doi:, 10.1016/j.bbagen.2020.129597. PMID:32156582<ref>PMID:32156582</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6wjo" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Arginine repressor 3D structures\|Arginine repressor 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Corynebacterium pseudotuberculosis C231]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Arni RK]]		[[Category: Arni RK]]

OCA: Protected "6wjo" [edit=sysop:move=sysop]

OCA — Wed, 22 Apr 2020 05:48:57 GMT

Protected "6wjo" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:48, 22 April 2020

OCA: New page: ==Crystal structure of wild-type Arginine Repressor from the pathogenic bacterium Corynebacterium pseudotuberculosis bound to tyrosine== == Structural highlights ==
<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WJO OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WJO FirstGlance]. <br>
</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wjo FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wjo OCA], [http://pdbe.org/6wjo PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wjo RCSB], [http://www.ebi.ac.uk/pdbsum/6wjo PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wjo ProSAT]</span></td></tr>
</table>
TOC
</StructureSection>
[[Category: Large Structures]]
[[Category: Arni RK]]
[[Category: Carareto CMA]]
[[Category: Hernandez-Gonzalez JE]]
[[Category: Mariutti RB]]
[[Category: Murakami MT]]
[[Category: Nascimento AFZ]]
[[Category: De Morais MAB]]

6wjo - Revision history

OCA at 14:27, 18 October 2023

OCA: Protected "6wjo" [edit=sysop:move=sysop]