6wl7 - Revision history

OCA at 14:45, 6 March 2024

OCA — Wed, 06 Mar 2024 14:45:02 GMT

←Older revision		Revision as of 14:45, 6 March 2024
Line 3:		Line 3:
	<StructureSection load='6wl7' size='340' side='right'caption='[[6wl7]], [[Resolution\|resolution]] 3.80Å' scene=''>		<StructureSection load='6wl7' size='340' side='right'caption='[[6wl7]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6wl7]] is a 150 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WL7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WL7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wl7]] is a 150 chain structure with sequence from [https://en.wikipedia.org/wiki/Synthetic_construct Synthetic construct]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WL7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WL7 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wl7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wl7 OCA], [https://pdbe.org/6wl7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wl7 RCSB], [https://www.ebi.ac.uk/pdbsum/6wl7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wl7 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 3.8Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wl7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wl7 OCA], [https://pdbe.org/6wl7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wl7 RCSB], [https://www.ebi.ac.uk/pdbsum/6wl7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wl7 ProSAT]</span></td></tr>
	</table>		</table>
-	<div style="background-color:#fffaf0;">
-	== Publication Abstract from PubMed ==
-	The exquisite structure-function correlations observed in filamentous protein assemblies provide a paradigm for the design of synthetic peptide-based nanomaterials. However, the plasticity of quaternary structure in sequence-space and the lability of helical symmetry present significant challenges to the de novo design and structural analysis of such filaments. Here, we describe a rational approach to design self-assembling peptide nanotubes based on controlling lateral interactions between protofilaments having an unusual cross-alpha supramolecular architecture. Near-atomic resolution cryo-EM structural analysis of seven designed nanotubes provides insight into the designability of interfaces within these synthetic peptide assemblies and identifies a non-native structural interaction based on a pair of arginine residues. This arginine clasp motif can robustly mediate cohesive interactions between protofilaments within the cross-alpha nanotubes. The structure of the resultant assemblies can be controlled through the sequence and length of the peptide subunits, which generates synthetic peptide filaments of similar dimensions to flagella and pili.
-
-	Structural analysis of cross alpha-helical nanotubes provides insight into the designability of filamentous peptide nanomaterials.,Wang F, Gnewou O, Modlin C, Beltran LC, Xu C, Su Z, Juneja P, Grigoryan G, Egelman EH, Conticello VP Nat Commun. 2021 Jan 18;12(1):407. doi: 10.1038/s41467-020-20689-w. PMID:33462223<ref>PMID:33462223</ref>
-
-	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
-	</div>
-	<div class="pdbe-citations 6wl7" style="background-color:#fffaf0;"></div>
-	== References ==
-	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Conticello~~, V P~~]]	+	[[Category: Synthetic construct]]
-	[[Category: Egelman~~, E H~~]]	+	[[Category: Conticello VP]]
-	[[Category: Gnewou~~, O M~~]]	+	[[Category: Egelman EH]]
-	[[Category: Modlin, C]]	+	[[Category: Gnewou OM]]
-	[[Category: Wang, F]]	+	[[Category: Modlin C]]
-	~~[[Category: Cryo-em]]~~	+	[[Category: Wang F]]
-	~~[[Category: Filament]]~~	+
-	~~[[Category: Protein fibril]]~~	+
-	~~[[Category: Self-assembly peptide filament~~]]	+

OCA at 14:52, 17 June 2021

OCA — Thu, 17 Jun 2021 14:52:34 GMT

←Older revision		Revision as of 14:52, 17 June 2021
Line 1:		Line 1:

	==Cryo-EM of Form 2 like peptide filament, 29-20-2==		==Cryo-EM of Form 2 like peptide filament, 29-20-2==
-	<StructureSection load='6wl7' size='340' side='right'caption='[[6wl7]]' scene=''>	+	<StructureSection load='6wl7' size='340' side='right'caption='[[6wl7]], [[Resolution\|resolution]] 3.80Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WL7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6WL7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wl7]] is a 150 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WL7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WL7 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6wl7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wl7 OCA], [~~http~~://pdbe.org/6wl7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6wl7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6wl7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6wl7 ProSAT]</span></td></tr>	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wl7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wl7 OCA], [https://pdbe.org/6wl7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wl7 RCSB], [https://www.ebi.ac.uk/pdbsum/6wl7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wl7 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	The exquisite structure-function correlations observed in filamentous protein assemblies provide a paradigm for the design of synthetic peptide-based nanomaterials. However, the plasticity of quaternary structure in sequence-space and the lability of helical symmetry present significant challenges to the de novo design and structural analysis of such filaments. Here, we describe a rational approach to design self-assembling peptide nanotubes based on controlling lateral interactions between protofilaments having an unusual cross-alpha supramolecular architecture. Near-atomic resolution cryo-EM structural analysis of seven designed nanotubes provides insight into the designability of interfaces within these synthetic peptide assemblies and identifies a non-native structural interaction based on a pair of arginine residues. This arginine clasp motif can robustly mediate cohesive interactions between protofilaments within the cross-alpha nanotubes. The structure of the resultant assemblies can be controlled through the sequence and length of the peptide subunits, which generates synthetic peptide filaments of similar dimensions to flagella and pili.
		+
		+	Structural analysis of cross alpha-helical nanotubes provides insight into the designability of filamentous peptide nanomaterials.,Wang F, Gnewou O, Modlin C, Beltran LC, Xu C, Su Z, Juneja P, Grigoryan G, Egelman EH, Conticello VP Nat Commun. 2021 Jan 18;12(1):407. doi: 10.1038/s41467-020-20689-w. PMID:33462223<ref>PMID:33462223</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6wl7" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Conticello VP]]	+	[[Category: Conticello, V P]]
-	[[Category: Egelman EH]]	+	[[Category: Egelman, E H]]
-	[[Category: Gnewou OM]]	+	[[Category: Gnewou, O M]]
-	[[Category: Modlin C]]	+	[[Category: Modlin, C]]
-	[[Category: Wang F]]	+	[[Category: Wang, F]]
		+	[[Category: Cryo-em]]
		+	[[Category: Filament]]
		+	[[Category: Protein fibril]]
		+	[[Category: Self-assembly peptide filament]]

OCA at 07:46, 2 December 2020

OCA — Wed, 02 Dec 2020 07:46:43 GMT

←Older revision		Revision as of 07:46, 2 December 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6wl7 ~~is ON HOLD until Paper Publication~~	+	==Cryo-EM of Form 2 like peptide filament, 29-20-2==
-		+	<StructureSection load='6wl7' size='340' side='right'caption='[[6wl7]]' scene=''>
-	~~Authors~~: ~~Wang, F~~., ~~Gnewou, O~~.M., ~~Modlin~~, C., ~~Egelman, E~~.H., ~~Conticello, V~~.P.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WL7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WL7 FirstGlance]. <br>
-	~~Description: Cryo-EM of Form 2 like peptide filament, 29-20-2~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wl7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wl7 OCA], [http://pdbe.org/6wl7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wl7 RCSB], [http://www.ebi.ac.uk/pdbsum/6wl7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wl7 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Egelman, E.H~~]]	+	__TOC__
-	[[Category: ~~Wang, F~~]]	+	</StructureSection>
-	[[Category: ~~Modlin, C~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Conticello, V.P~~]]	+	[[Category: Conticello VP]]
-	[[Category: ~~Gnewou, O.M~~]]	+	[[Category: Egelman EH]]
		+	[[Category: Gnewou OM]]
		+	[[Category: Modlin C]]
		+	[[Category: Wang F]]

OCA at 04:13, 20 May 2020

OCA — Wed, 20 May 2020 04:13:52 GMT

←Older revision		Revision as of 04:13, 20 May 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6wl7 is ON HOLD	+	The entry 6wl7 is ON HOLD until Paper Publication

	Authors: Wang, F., Gnewou, O.M., Modlin, C., Egelman, E.H., Conticello, V.P.		Authors: Wang, F., Gnewou, O.M., Modlin, C., Egelman, E.H., Conticello, V.P.
Line 7:		Line 7:
	Description: Cryo-EM of Form 2 like peptide filament, 29-20-2		Description: Cryo-EM of Form 2 like peptide filament, 29-20-2
	[[Category: Unreleased Structures]]		[[Category: Unreleased Structures]]
-	[[Category: Conticello, V.P]]
	[[Category: Egelman, E.H]]		[[Category: Egelman, E.H]]
		+	[[Category: Wang, F]]
	[[Category: Modlin, C]]		[[Category: Modlin, C]]
		+	[[Category: Conticello, V.P]]
	[[Category: Gnewou, O.M]]		[[Category: Gnewou, O.M]]
-	[[Category: Wang, F]]

OCA: Protected "6wl7" [edit=sysop:move=sysop]

OCA — Wed, 29 Apr 2020 05:14:06 GMT

Protected "6wl7" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:14, 29 April 2020

OCA: New page: '''Unreleased structure''' The entry 6wl7 is ON HOLD Authors: Wang, F., Gnewou, O.M., Modlin, C., Egelman, E.H., Conticello, V.P. Description: Cryo-EM of Form 2 like peptide filament, ...

OCA — Wed, 29 Apr 2020 05:14:05 GMT

New page: '''Unreleased structure''' The entry 6wl7 is ON HOLD Authors: Wang, F., Gnewou, O.M., Modlin, C., Egelman, E.H., Conticello, V.P. Description: Cryo-EM of Form 2 like peptide filament, ...

New page

'''Unreleased structure'''

The entry 6wl7 is ON HOLD

Authors: Wang, F., Gnewou, O.M., Modlin, C., Egelman, E.H., Conticello, V.P.

Description: Cryo-EM of Form 2 like peptide filament, 29-20-2
[[Category: Unreleased Structures]]
[[Category: Conticello, V.P]]
[[Category: Egelman, E.H]]
[[Category: Modlin, C]]
[[Category: Gnewou, O.M]]
[[Category: Wang, F]]