OCA at 14:30, 18 October 2023

OCA — Wed, 18 Oct 2023 14:30:55 GMT

←Older revision		Revision as of 14:30, 18 October 2023
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-	====	+	==Crystal structure of human SFPQ/NONO complex==
-	<StructureSection load='6wmz' size='340' side='right'caption='[[6wmz]]' scene=''>	+	<StructureSection load='6wmz' size='340' side='right'caption='[[6wmz]], [[Resolution\|resolution]] 2.85Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wmz]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WMZ OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WMZ FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wmz OCA], [https://pdbe.org/6wmz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wmz RCSB], [https://www.ebi.ac.uk/pdbsum/6wmz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wmz ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.85Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wmz OCA], [https://pdbe.org/6wmz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wmz RCSB], [https://www.ebi.ac.uk/pdbsum/6wmz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wmz ProSAT]</span></td></tr>
	</table>		</table>
		+	== Disease ==
		+	[https://www.uniprot.org/uniprot/SFPQ_HUMAN SFPQ_HUMAN] Translocation renal cell carcinoma. A chromosomal aberration involving SFPQ may be a cause of papillary renal cell carcinoma (PRCC). Translocation t(X;1)(p11.2;p34) with TFE3.
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SFPQ_HUMAN SFPQ_HUMAN] DNA- and RNA binding protein, involved in several nuclear processes. Essential pre-mRNA splicing factor required early in spliceosome formation and for splicing catalytic step II, probably as a heteromer with NONO. Binds to pre-mRNA in spliceosome C complex, and specifically binds to intronic polypyrimidine tracts. Involved in regulation of signal-induced alternative splicing. During splicing of PTPRC/CD45, a phosphorylated form is sequestered by THRAP3 from the pre-mRNA in resting T-cells; T-cell activation and subsequent reduced phosphorylation is proposed to lead to release from THRAP3 allowing binding to pre-mRNA splicing regulatotry elements which represses exon inclusion. Interacts with U5 snRNA, probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. May be involved in a pre-mRNA coupled splicing and polyadenylation process as component of a snRNP-free complex with SNRPA/U1A. The SFPQ-NONO heteromer associated with MATR3 may play a role in nuclear retention of defective RNAs. SFPQ may be involved in homologous DNA pairing; in vitro, promotes the invasion of ssDNA between a duplex DNA and produces a D-loop formation. The SFPQ-NONO heteromer may be involved in DNA unwinding by modulating the function of topoisomerase I/TOP1; in vitro, stimulates dissociation of TOP1 from DNA after cleavage and enhances its jumping between separate DNA helices. The SFPQ-NONO heteromer may be involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination and may stabilize paired DNA ends; in vitro, the complex strongly stimulates DNA end joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation complex. SFPQ is involved in transcriptional regulation. Transcriptional repression is mediated by an interaction of SFPQ with SIN3A and subsequent recruitment of histone deacetylases (HDACs). The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter and regulates basal and cAMP-dependent transcriptional avtivity. SFPQ isoform Long binds to the DNA binding domains (DBD) of nuclear hormone receptors, like RXRA and probably THRA, and acts as transcriptional corepressor in absence of hormone ligands. Binds the DNA sequence 5'-CTGAGTC-3' in the insulin-like growth factor response element (IGFRE) and inhibits IGF-I-stimulated transcriptional activity. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Required for the transcriptional repression of circadian target genes, such as PER1, mediated by the large PER complex through histone deacetylation.<ref>PMID:10847580</ref> <ref>PMID:10858305</ref> <ref>PMID:10931916</ref> <ref>PMID:11259580</ref> <ref>PMID:11525732</ref> <ref>PMID:11897684</ref> <ref>PMID:15590677</ref> <ref>PMID:20932480</ref> <ref>PMID:8045264</ref> <ref>PMID:8449401</ref>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Bond CS]]
		+	[[Category: Lee M]]

OCA at 05:17, 28 April 2021

OCA — Wed, 28 Apr 2021 05:17:42 GMT

←Older revision		Revision as of 05:17, 28 April 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6wmz ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6wmz' size='340' side='right'caption='[[6wmz]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wmz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wmz OCA], [https://pdbe.org/6wmz PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wmz RCSB], [https://www.ebi.ac.uk/pdbsum/6wmz PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wmz ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 04:24, 6 May 2020

OCA — Wed, 06 May 2020 04:24:28 GMT

←Older revision		Revision as of 04:24, 6 May 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6wmz is ON HOLD	+	The entry 6wmz is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6wmz" [edit=sysop:move=sysop]

OCA — Wed, 29 Apr 2020 05:18:54 GMT

Protected "6wmz" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:18, 29 April 2020

OCA: New page: '''Unreleased structure''' The entry 6wmz is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 29 Apr 2020 05:18:53 GMT

New page: '''Unreleased structure''' The entry 6wmz is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6wmz is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6wmz - Revision history

OCA at 14:30, 18 October 2023

OCA at 05:17, 28 April 2021

OCA at 04:24, 6 May 2020

OCA: Protected "6wmz" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6wmz is ON HOLD Authors: Description: Category: Unreleased Structures