OCA at 14:33, 18 October 2023

OCA — Wed, 18 Oct 2023 14:33:34 GMT

←Older revision		Revision as of 14:33, 18 October 2023
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	==GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis with bound cellotriose==		==GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis with bound cellotriose==
-	<StructureSection load='6wqv' size='340' side='right'caption='[[6wqv]]' scene=''>	+	<StructureSection load='6wqv' size='340' side='right'caption='[[6wqv]], [[Resolution\|resolution]] 1.45Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WQV OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6WQV FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wqv]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Ruminococcus_champanellensis Ruminococcus champanellensis]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WQV OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WQV FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6wqv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wqv OCA], [~~http~~://pdbe.org/6wqv PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6wqv RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6wqv PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6wqv ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 1.45Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BGC:BETA-D-GLUCOSE'>BGC</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GLC:ALPHA-D-GLUCOSE'>GLC</scene>, <scene name='pdbligand=NO3:NITRATE+ION'>NO3</scene>, <scene name='pdbligand=PRD_900014:alpha-cellotriose'>PRD_900014</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wqv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wqv OCA], [https://pdbe.org/6wqv PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wqv RCSB], [https://www.ebi.ac.uk/pdbsum/6wqv PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wqv ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/D4LAX7_RUMC1 D4LAX7_RUMC1]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Broad-specificity glycoside hydrolases (GHs) contribute to plant biomass hydrolysis by degrading a diverse range of polysaccharides, making them useful catalysts for renewable energy and biocommodity production. Discovery of new GHs with improved kinetic parameters or more tolerant substrate-binding sites could increase the efficiency of renewable bioenergy production even further. GH5 has over 50 subfamilies exhibiting selectivities for reaction with beta-(1,4)-linked oligo- and polysaccharides. Among these, subfamily 4 (GH5_4) contains numerous broad-selectivity endoglucanases that hydrolyze cellulose, xyloglucan, and mixed-linkage glucans. We previously surveyed the whole subfamily and found over 100 new broad-specificity endoglucanases, although the structural origins of broad specificity remained unclear. A mechanistic understanding of GH5_4 substrate specificity would help inform the best protein design strategies and the most appropriate industrial application of broad-specificity endoglucanases. Here we report structures of 10 new GH5_4 enzymes from cellulolytic microbes and characterize their substrate selectivity using normalized reducing sugar assays and MS. We found that GH5_4 enzymes have the highest catalytic efficiency for hydrolysis of xyloglucan, glucomannan, and soluble beta-glucans, with opportunistic secondary reactions on cellulose, mannan, and xylan. The positions of key aromatic residues determine the overall reaction rate and breadth of substrate tolerance, and they contribute to differences in oligosaccharide cleavage patterns. Our new composite model identifies several critical structural features that confer broad specificity and may be readily engineered into existing industrial enzymes. We demonstrate that GH5_4 endoglucanases can have broad specificity without sacrificing high activity, making them a valuable addition to the biomass deconstruction toolset.
		+
		+	A structural and kinetic survey of GH5_4 endoglucanases reveals determinants of broad substrate specificity and opportunities for biomass hydrolysis.,Glasgow EM, Kemna EI, Bingman CA, Ing N, Deng K, Bianchetti CM, Takasuka TE, Northen TR, Fox BG J Biol Chem. 2020 Dec 18;295(51):17752-17769. doi: 10.1074/jbc.RA120.015328. PMID:33454012<ref>PMID:33454012</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6wqv" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Glucanase 3D structures\|Glucanase 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
		+	[[Category: Ruminococcus champanellensis]]
	[[Category: Bianchetti CM]]		[[Category: Bianchetti CM]]
	[[Category: Bingman CA]]		[[Category: Bingman CA]]

OCA at 09:37, 18 November 2020

OCA — Wed, 18 Nov 2020 09:37:08 GMT

←Older revision		Revision as of 09:37, 18 November 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6wqv is ~~ON HOLD until Paper Publication~~	+	==GH5-4 broad specificity endoglucanase from Ruminococcus champanellensis with bound cellotriose==
-		+	<StructureSection load='6wqv' size='340' side='right'caption='[[6wqv]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WQV OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WQV FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wqv FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wqv OCA], [http://pdbe.org/6wqv PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wqv RCSB], [http://www.ebi.ac.uk/pdbsum/6wqv PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wqv ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Bianchetti CM]]
		+	[[Category: Bingman CA]]
		+	[[Category: Fox BG]]
		+	[[Category: Glasgow EM]]
		+	[[Category: Smith RW]]

OCA at 05:09, 3 June 2020

OCA — Wed, 03 Jun 2020 05:09:31 GMT

←Older revision		Revision as of 05:09, 3 June 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6wqv is ON HOLD	+	The entry 6wqv is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6wqv" [edit=sysop:move=sysop]

OCA — Wed, 06 May 2020 04:32:25 GMT

Protected "6wqv" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:32, 6 May 2020

OCA: New page: '''Unreleased structure''' The entry 6wqv is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 06 May 2020 04:32:24 GMT

New page: '''Unreleased structure''' The entry 6wqv is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6wqv is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6wqv - Revision history

OCA at 14:33, 18 October 2023

OCA at 09:37, 18 November 2020

OCA at 05:09, 3 June 2020

OCA: Protected "6wqv" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6wqv is ON HOLD Authors: Description: Category: Unreleased Structures