6wt7 - Revision history

OCA at 14:46, 6 March 2024

OCA — Wed, 06 Mar 2024 14:46:45 GMT

←Older revision		Revision as of 14:46, 6 March 2024
Line 3:		Line 3:
	<StructureSection load='6wt7' size='340' side='right'caption='[[6wt7]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='6wt7' size='340' side='right'caption='[[6wt7]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6wt7]] is a 1 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/~~Cragi Cragi~~]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WT7 OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6WT7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wt7]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Crassostrea_gigas Crassostrea gigas]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WT7 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WT7 FirstGlance]. <br>
-	</td></tr><tr id='~~ligand~~'><td class="sblockLbl"><b>[[~~Ligand~~\|~~Ligands~~:]]</b></td><td class="sblockDat" id="~~ligandDat~~">~~<scene name='pdbligand=1SY:CGAMP'>1SY</scene>~~</td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
-	<tr id='~~gene~~'><td class="sblockLbl"><b>[[~~Gene~~\|~~Gene~~:]]</b></td><td class="sblockDat">~~XP_011430837.1 ([http~~:/~~/www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29159 CRAGI])~~</td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1SY:CGAMP'>1SY</scene></td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6wt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wt7 OCA], [~~http~~://pdbe.org/6wt7 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6wt7 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6wt7 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6wt7 ProSAT]</span></td></tr>	+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wt7 OCA], [https://pdbe.org/6wt7 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wt7 RCSB], [https://www.ebi.ac.uk/pdbsum/6wt7 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wt7 ProSAT]</span></td></tr>
	</table>		</table>
-	~~<div style~~=~~"background-color:#fffaf0;">~~	+	== Function ==
-	=~~= Publication Abstract from PubMed~~ ==	+	[https://www.uniprot.org/uniprot/STING_CRAGI STING_CRAGI]
-	Stimulator of interferon genes (STING) is a receptor in human cells that senses foreign cyclic dinucleotides released during bacterial infection and endogenous cyclic GMP-AMP signalling during viral infection and antitumour immunity(1-5). STING shares no structural homology with other known signalling proteins(6-9), limiting functional analysis and preventing explanation of the origin of cyclic dinucleotide signalling in mammalian innate immunity. Here we discover functional STING homologues encoded within prokaryotic defence islands and reveal a conserved mechanism of signal activation. Crystal structures of bacterial STING define a minimal homodimeric scaffold that selectively responds to c-di-GMP synthesized by a neighbouring cGAS/DncV-like nucleotidyltransferase (CD-NTase) enzyme. Bacterial STING domains couple cyclic dinucleotide recognition with protein filament formation to drive TIR effector domain oligomerization and rapid NAD(+) cleavage. We reconstruct the evolutionary events following acquisition of STING into metazoan innate immunity and determine the structure of a full-length TIR-STING fusion from the Pacific oyster Crassostrea gigas. Comparative structural analysis demonstrates how metazoan-specific additions to the core STING scaffold enabled a switch from direct effector function to regulation of antiviral transcription. Together, our results explain the mechanism of STING-dependent signalling and reveal conservation of a functional cGAS-STING pathway in prokaryotic bacteriophage defence.	+
-		+
-	~~STING cyclic dinucleotide sensing originated in bacteria.,Morehouse BR, Govande AA, Millman A, Keszei AFA, Lowey B, Ofir G, Shao S, Sorek R, Kranzusch PJ Nature. 2020 Sep 2. pii~~: ~~10.1038~~/~~s41586-020-2719-5. doi:, 10.1038~~/~~s41586-020-2719-5~~. ~~PMID:32877915<ref>PMID:32877915</ref>~~	+
-		+
-	~~From MEDLINE®/PubMed®, a database of the U~~.~~S. National Library of Medicine.<br>~~	+
-	</~~div>~~	+
-	~~<div class="pdbe-citations 6wt7" style="background-color:#fffaf0;"></div>~~	+
-	~~== References ==~~	+
-	~~<references~~/>	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
-	[[Category: ~~Cragi~~]]	+	[[Category: Crassostrea gigas]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Govande~~, A A~~]]	+	[[Category: Govande AA]]
-	[[Category: Keszei~~, A F.A~~]]	+	[[Category: Keszei AFA]]
-	[[Category: Kranzusch~~, P J~~]]	+	[[Category: Kranzusch PJ]]
-	[[Category: Lowey, B]]	+	[[Category: Lowey B]]
-	[[Category: Millman, A]]	+	[[Category: Millman A]]
-	[[Category: Morehouse~~, B R~~]]	+	[[Category: Morehouse BR]]
-	[[Category: Ofir, G]]	+	[[Category: Ofir G]]
-	[[Category: Shao, S]]	+	[[Category: Shao S]]
-	[[Category: Sorek, R]]	+	[[Category: Sorek R]]
-	~~[[Category: Cyclic dinucleotide receptor]]~~	+
-	~~[[Category: Immune effector]]~~	+
-	~~[[Category: Immune system]]~~	+
-	~~[[Category: Tir domain~~]]	+

OCA at 10:17, 16 September 2020

OCA — Wed, 16 Sep 2020 10:17:04 GMT

←Older revision		Revision as of 10:17, 16 September 2020
Line 1:		Line 1:

	==Structure of a metazoan TIR-STING receptor from C. gigas in complex with 2',3'-cGAMP==		==Structure of a metazoan TIR-STING receptor from C. gigas in complex with 2',3'-cGAMP==
-	<StructureSection load='6wt7' size='340' side='right'caption='[[6wt7]]' scene=''>	+	<StructureSection load='6wt7' size='340' side='right'caption='[[6wt7]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WT7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WT7 FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wt7]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Cragi Cragi]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WT7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WT7 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wt7 OCA], [http://pdbe.org/6wt7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wt7 RCSB], [http://www.ebi.ac.uk/pdbsum/6wt7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wt7 ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=1SY:CGAMP'>1SY</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">XP_011430837.1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=29159 CRAGI])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wt7 OCA], [http://pdbe.org/6wt7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wt7 RCSB], [http://www.ebi.ac.uk/pdbsum/6wt7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wt7 ProSAT]</span></td></tr>
	</table>		</table>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Stimulator of interferon genes (STING) is a receptor in human cells that senses foreign cyclic dinucleotides released during bacterial infection and endogenous cyclic GMP-AMP signalling during viral infection and antitumour immunity(1-5). STING shares no structural homology with other known signalling proteins(6-9), limiting functional analysis and preventing explanation of the origin of cyclic dinucleotide signalling in mammalian innate immunity. Here we discover functional STING homologues encoded within prokaryotic defence islands and reveal a conserved mechanism of signal activation. Crystal structures of bacterial STING define a minimal homodimeric scaffold that selectively responds to c-di-GMP synthesized by a neighbouring cGAS/DncV-like nucleotidyltransferase (CD-NTase) enzyme. Bacterial STING domains couple cyclic dinucleotide recognition with protein filament formation to drive TIR effector domain oligomerization and rapid NAD(+) cleavage. We reconstruct the evolutionary events following acquisition of STING into metazoan innate immunity and determine the structure of a full-length TIR-STING fusion from the Pacific oyster Crassostrea gigas. Comparative structural analysis demonstrates how metazoan-specific additions to the core STING scaffold enabled a switch from direct effector function to regulation of antiviral transcription. Together, our results explain the mechanism of STING-dependent signalling and reveal conservation of a functional cGAS-STING pathway in prokaryotic bacteriophage defence.
		+
		+	STING cyclic dinucleotide sensing originated in bacteria.,Morehouse BR, Govande AA, Millman A, Keszei AFA, Lowey B, Ofir G, Shao S, Sorek R, Kranzusch PJ Nature. 2020 Sep 2. pii: 10.1038/s41586-020-2719-5. doi:, 10.1038/s41586-020-2719-5. PMID:32877915<ref>PMID:32877915</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6wt7" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Cragi]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Govande AA]]	+	[[Category: Govande, A A]]
-	[[Category: Keszei ~~AFA~~]]	+	[[Category: Keszei, A F.A]]
-	[[Category: Kranzusch PJ]]	+	[[Category: Kranzusch, P J]]
-	[[Category: Lowey B]]	+	[[Category: Lowey, B]]
-	[[Category: Millman A]]	+	[[Category: Millman, A]]
-	[[Category: Morehouse BR]]	+	[[Category: Morehouse, B R]]
-	[[Category: Ofir G]]	+	[[Category: Ofir, G]]
-	[[Category: Shao S]]	+	[[Category: Shao, S]]
-	[[Category: Sorek R]]	+	[[Category: Sorek, R]]
		+	[[Category: Cyclic dinucleotide receptor]]
		+	[[Category: Immune effector]]
		+	[[Category: Immune system]]
		+	[[Category: Tir domain]]

OCA at 09:34, 9 September 2020

OCA — Wed, 09 Sep 2020 09:34:00 GMT

←Older revision		Revision as of 09:34, 9 September 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6wt7 is ~~ON HOLD until Paper Publication~~	+	==Structure of a metazoan TIR-STING receptor from C. gigas in complex with 2',3'-cGAMP==
-		+	<StructureSection load='6wt7' size='340' side='right'caption='[[6wt7]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WT7 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WT7 FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wt7 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wt7 OCA], [http://pdbe.org/6wt7 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wt7 RCSB], [http://www.ebi.ac.uk/pdbsum/6wt7 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wt7 ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Govande AA]]
		+	[[Category: Keszei AFA]]
		+	[[Category: Kranzusch PJ]]
		+	[[Category: Lowey B]]
		+	[[Category: Millman A]]
		+	[[Category: Morehouse BR]]
		+	[[Category: Ofir G]]
		+	[[Category: Shao S]]
		+	[[Category: Sorek R]]

OCA at 04:15, 20 May 2020

OCA — Wed, 20 May 2020 04:15:15 GMT

←Older revision		Revision as of 04:15, 20 May 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6wt7 is ON HOLD	+	The entry 6wt7 is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6wt7" [edit=sysop:move=sysop]

OCA — Wed, 13 May 2020 04:06:28 GMT

Protected "6wt7" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:06, 13 May 2020

OCA: New page: '''Unreleased structure''' The entry 6wt7 is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 13 May 2020 04:06:27 GMT

New page: '''Unreleased structure''' The entry 6wt7 is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6wt7 is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]