6wts - Revision history

OCA at 05:59, 5 August 2020

OCA — Wed, 05 Aug 2020 05:59:11 GMT

←Older revision		Revision as of 05:59, 5 August 2020
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	==CryoEM structure of the C. sordellii lethal toxin TcsL in complex with SEMA6A==		==CryoEM structure of the C. sordellii lethal toxin TcsL in complex with SEMA6A==
-	<StructureSection load='6wts' size='340' side='right'caption='[[6wts]]' scene=''>	+	<StructureSection load='6wts' size='340' side='right'caption='[[6wts]], [[Resolution\|resolution]] 3.30Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WTS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WTS FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wts]] is a 3 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_oedematis_sporogenes"_sordelli_1923 "bacillus oedematis sporogenes" sordelli 1923] and [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WTS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WTS FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wts OCA], [http://pdbe.org/6wts PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wts RCSB], [http://www.ebi.ac.uk/pdbsum/6wts PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wts ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='gene'><td class="sblockLbl"><b>[[Gene\|Gene:]]</b></td><td class="sblockDat">SEMA6A, KIAA1368, SEMAQ ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), tcsL, JGS6382_PCS1300041 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1505 "Bacillus oedematis sporogenes" Sordelli 1923])</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wts OCA], [http://pdbe.org/6wts PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wts RCSB], [http://www.ebi.ac.uk/pdbsum/6wts PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wts ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/SEM6A_HUMAN SEM6A_HUMAN]] Cell surface receptor for PLXNA2 that plays an important role in cell-cell signaling. Required for normal granule cell migration in the developing cerebellum. Promotes reorganization of the actin cytoskeleton and plays an important role in axon guidance in the developing central nervous system. Can act as repulsive axon guidance cue. Has repulsive action towards migrating granular neurons. May play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation (By similarity).
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Pathogenic clostridial species secrete potent toxins that induce severe host tissue damage. Paeniclostridium sordellii lethal toxin (TcsL) causes an almost invariably lethal toxic shock syndrome associated with gynecological infections. TcsL is 87% similar to C. difficile TcdB, which enters host cells via Frizzled receptors in colon epithelium. However, P. sordellii infections target vascular endothelium, suggesting that TcsL exploits another receptor. Here, using CRISPR/Cas9 screening, we establish semaphorins SEMA6A and SEMA6B as TcsL receptors. We demonstrate that recombinant SEMA6A can protect mice from TcsL-induced edema. A 3.3 A cryo-EM structure shows that TcsL binds SEMA6A with the same region that in TcdB binds structurally unrelated Frizzled. Remarkably, 15 mutations in this evolutionarily divergent surface are sufficient to switch binding specificity of TcsL to that of TcdB. Our findings establish semaphorins as physiologically relevant receptors for TcsL and reveal the molecular basis for the difference in tissue targeting and disease pathogenesis between highly related toxins.
		+
		+	Recognition of Semaphorin Proteins by P. sordellii Lethal Toxin Reveals Principles of Receptor Specificity in Clostridial Toxins.,Lee H, Beilhartz GL, Kucharska I, Raman S, Cui H, Lam MHY, Liang H, Rubinstein JL, Schramek D, Julien JP, Melnyk RA, Taipale M Cell. 2020 Jun 23. pii: S0092-8674(20)30692-9. doi: 10.1016/j.cell.2020.06.005. PMID:32589945<ref>PMID:32589945</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6wts" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bacillus oedematis sporogenes sordelli 1923]]
		+	[[Category: Human]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Julien JP]]	+	[[Category: Julien, J P]]
-	[[Category: Kucharska I]]	+	[[Category: Kucharska, I]]
-	[[Category: Rubinstein JL]]	+	[[Category: Rubinstein, J L]]
		+	[[Category: Cryoem]]
		+	[[Category: Signaling protein-toxin complex]]

OCA at 14:10, 8 July 2020

OCA — Wed, 08 Jul 2020 14:10:12 GMT

←Older revision		Revision as of 14:10, 8 July 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6wts is ~~ON HOLD until Paper Publication~~	+	==CryoEM structure of the C. sordellii lethal toxin TcsL in complex with SEMA6A==
-		+	<StructureSection load='6wts' size='340' side='right'caption='[[6wts]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WTS OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WTS FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wts FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wts OCA], [http://pdbe.org/6wts PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wts RCSB], [http://www.ebi.ac.uk/pdbsum/6wts PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wts ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased Structures~~]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Julien JP]]
		+	[[Category: Kucharska I]]
		+	[[Category: Rubinstein JL]]

OCA at 18:16, 24 June 2020

OCA — Wed, 24 Jun 2020 18:16:55 GMT

←Older revision		Revision as of 18:16, 24 June 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6wts is ON HOLD	+	The entry 6wts is ON HOLD until Paper Publication

	Authors:		Authors:

OCA at 08:33, 17 June 2020

OCA — Wed, 17 Jun 2020 08:33:08 GMT

←Older revision		Revision as of 08:33, 17 June 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6wts is ON HOLD ~~until Paper Publication~~	+	The entry 6wts is ON HOLD

	Authors:		Authors:

OCA at 04:51, 10 June 2020

OCA — Wed, 10 Jun 2020 04:51:45 GMT

←Older revision		Revision as of 04:51, 10 June 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6wts is ON HOLD	+	The entry 6wts is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6wts" [edit=sysop:move=sysop]

OCA — Wed, 13 May 2020 04:07:52 GMT

Protected "6wts" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:07, 13 May 2020

OCA: New page: '''Unreleased structure''' The entry 6wts is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 13 May 2020 04:07:50 GMT

New page: '''Unreleased structure''' The entry 6wts is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6wts is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]