OCA at 14:37, 18 October 2023

OCA — Wed, 18 Oct 2023 14:37:51 GMT

←Older revision		Revision as of 14:37, 18 October 2023
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	==E.coli DsbB C104S with ubiquinone==		==E.coli DsbB C104S with ubiquinone==
-	<StructureSection load='6wvf' size='340' side='right'caption='[[6wvf]]' scene=''>	+	<StructureSection load='6wvf' size='340' side='right'caption='[[6wvf]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WVF OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6WVF FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wvf]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Aequorea_victoria Aequorea victoria] and [https://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WVF OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WVF FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6wvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wvf OCA], [~~http~~://pdbe.org/6wvf PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6wvf RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6wvf PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6wvf ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.9Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CRO:{2-[(1R,2R)-1-AMINO-2-HYDROXYPROPYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL}ACETIC+ACID'>CRO</scene>, <scene name='pdbligand=UQ1:UBIQUINONE-1'>UQ1</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wvf OCA], [https://pdbe.org/6wvf PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wvf RCSB], [https://www.ebi.ac.uk/pdbsum/6wvf PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wvf ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/DSBB_ECOLI DSBB_ECOLI] Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by oxidizing the DsbA protein.<ref>PMID:8430071</ref> <ref>PMID:7688471</ref> [https://www.uniprot.org/uniprot/GFP_AEQVI GFP_AEQVI] Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Small membrane proteins are difficult targets for structural characterization. Here, we stabilize their folding by restraining their amino and carboxyl termini with associable protein entities, exemplified by the two halves of a superfolder GFP. The termini-restrained proteins are functional and show improved stability during overexpression and purification. The reassembled GFP provides a versatile scaffold for membrane protein crystallization, enables diffraction to atomic resolution, and facilitates crystal identification, phase determination, and density modification. This strategy gives rise to 14 new structures of five vertebrate proteins from distinct functional families, bringing a substantial expansion to the structural database of small membrane proteins. Moreover, a high-resolution structure of bacterial DsbB reveals that this thiol oxidoreductase is activated through a catalytic triad, similar to cysteine proteases. Overall, termini restraining proves exceptionally effective for stabilization and structure determination of small membrane proteins.
		+
		+	Termini restraining of small membrane proteins enables structure determination at near-atomic resolution.,Liu S, Li S, Yang Y, Li W Sci Adv. 2020 Dec 18;6(51). pii: 6/51/eabe3717. doi: 10.1126/sciadv.abe3717., Print 2020 Dec. PMID:33355146<ref>PMID:33355146</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6wvf" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Green Fluorescent Protein 3D structures\|Green Fluorescent Protein 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Aequorea victoria]]
		+	[[Category: Escherichia coli]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
	[[Category: Li W]]		[[Category: Li W]]
	[[Category: Liu S]]		[[Category: Liu S]]

OCA at 05:49, 20 January 2021

OCA — Wed, 20 Jan 2021 05:49:49 GMT

←Older revision		Revision as of 05:49, 20 January 2021
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-	'''Unreleased structure'''

-	~~The entry~~ 6wvf ~~is ON HOLD until May 05 2022~~	+	==E.coli DsbB C104S with ubiquinone==
-		+	<StructureSection load='6wvf' size='340' side='right'caption='[[6wvf]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WVF OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WVF FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wvf FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wvf OCA], [http://pdbe.org/6wvf PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wvf RCSB], [http://www.ebi.ac.uk/pdbsum/6wvf PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wvf ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Li W]]
		+	[[Category: Liu S]]

OCA at 06:30, 22 July 2020

OCA — Wed, 22 Jul 2020 06:30:10 GMT

←Older revision		Revision as of 06:30, 22 July 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6wvf is ON HOLD	+	The entry 6wvf is ON HOLD until May 05 2022

	Authors:		Authors:

OCA: Protected "6wvf" [edit=sysop:move=sysop]

OCA — Wed, 13 May 2020 04:11:14 GMT

Protected "6wvf" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:11, 13 May 2020

OCA: New page: '''Unreleased structure''' The entry 6wvf is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 13 May 2020 04:11:13 GMT

New page: '''Unreleased structure''' The entry 6wvf is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6wvf is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6wvf - Revision history

OCA at 14:37, 18 October 2023

OCA at 05:49, 20 January 2021

OCA at 06:30, 22 July 2020

OCA: Protected "6wvf" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6wvf is ON HOLD Authors: Description: Category: Unreleased Structures