6wvr - Revision history

OCA at 14:47, 6 March 2024

OCA — Wed, 06 Mar 2024 14:47:19 GMT

←Older revision		Revision as of 14:47, 6 March 2024
Line 3:		Line 3:
	<StructureSection load='6wvr' size='340' side='right'caption='[[6wvr]], [[Resolution\|resolution]] 2.90Å' scene=''>		<StructureSection load='6wvr' size='340' side='right'caption='[[6wvr]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>[[6wvr]] is a 4 chain structure with sequence from [~~http~~://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WVR OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol=6WVR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wvr]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WVR OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6WVR FirstGlance]. <br>
-	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">Electron Microscopy, [[Resolution\|Resolution]] 2.9Å</td></tr>
-	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6wvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wvr OCA], [~~http~~://pdbe.org/6wvr PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6wvr RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6wvr PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6wvr ProSAT]</span></td></tr>	+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6wvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wvr OCA], [https://pdbe.org/6wvr PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6wvr RCSB], [https://www.ebi.ac.uk/pdbsum/6wvr PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6wvr ProSAT]</span></td></tr>
	</table>		</table>
	== Function ==		== Function ==
-	[~~[http~~://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/E1BJB1_BOVIN E1BJB1_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.[RuleBase:RU000352][SAAS:SAAS00031082]	+	[https://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.
-	~~<div style="background-color:#fffaf0;">~~	+
-	~~== Publication Abstract from PubMed ==~~	+
-	Microtubules are tubular polymers with essential roles in numerous cellular activities. Structures of microtubules have been captured at increasing resolution by cryo-EM. However, dynamic properties of the microtubule are key to its function, and this behavior has proved difficult to characterize at a structural level due to limitations in existing structure determination methods. We developed a high-resolution cryo-EM refinement method that divides an imaged microtubule into its constituent protofilaments, enabling deviations from helicity and other sources of heterogeneity to be quantified and corrected for at the single-subunit level. We demonstrate that this method improves the resolution of microtubule 3D reconstructions and substantially reduces anisotropic blurring artifacts, compared with methods that utilize helical symmetry averaging. Moreover, we identified an unexpected, discrete behavior of the m-loop, which mediates lateral interactions between neighboring protofilaments and acts as a flexible hinge between them. The hinge angle adopts preferred values corresponding to distinct conformations of the m-loop that are incompatible with helical symmetry. These hinge angles fluctuate in a stochastic manner, and perfectly cylindrical microtubule conformations are thus energetically and entropically penalized. The hinge angle can diverge further from helical symmetry at the microtubule seam, generating a subpopulation of highly distorted microtubules. However, the seam-distorted subpopulation disappears in the presence of Taxol, a microtubule stabilizing agent. These observations provide clues into the structural origins of microtubule flexibility and dynamics and highlight the role of structural polymorphism in defining microtubule behavior.	+

-	Dynamic and asymmetric fluctuations in the microtubule wall captured by high-resolution cryoelectron microscopy.,Debs GE, Cha M, Liu X, Huehn AR, Sindelar CV Proc Natl Acad Sci U S A. 2020 Jul 7. pii: 2001546117. doi:, 10.1073/pnas.2001546117. PMID:32636254<ref>PMID:32636254</ref>	+	==See Also==
-		+	*[[Tubulin 3D Structures\|Tubulin 3D Structures]]
-	~~From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>~~	+
-	~~</div>~~	+
-	~~<div class="pdbe-citations 6wvr" style="background-color:#fffaf0;"></div>~~	+
-	== ~~References~~ ==	+
-	~~<references/>~~	+
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Bos taurus]]		[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cha, M]]	+	[[Category: Cha M]]
-	[[Category: Debs~~, G E~~]]	+	[[Category: Debs GE]]
-	[[Category: Huehn~~, A R~~]]	+	[[Category: Huehn AR]]
-	[[Category: Sindelar~~, C V]]~~	+	[[Category: Sindelar CV]]
-	~~[[Category: Microtubule]]~~	+
-	~~[[Category: Structural protein~~]]	+

OCA at 11:45, 22 July 2020

OCA — Wed, 22 Jul 2020 11:45:39 GMT

←Older revision		Revision as of 11:45, 22 July 2020
Line 9:		Line 9:
	== Function ==		== Function ==
	[[http://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/E1BJB1_BOVIN E1BJB1_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.[RuleBase:RU000352][SAAS:SAAS00031082]		[[http://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/E1BJB1_BOVIN E1BJB1_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.[RuleBase:RU000352][SAAS:SAAS00031082]
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Microtubules are tubular polymers with essential roles in numerous cellular activities. Structures of microtubules have been captured at increasing resolution by cryo-EM. However, dynamic properties of the microtubule are key to its function, and this behavior has proved difficult to characterize at a structural level due to limitations in existing structure determination methods. We developed a high-resolution cryo-EM refinement method that divides an imaged microtubule into its constituent protofilaments, enabling deviations from helicity and other sources of heterogeneity to be quantified and corrected for at the single-subunit level. We demonstrate that this method improves the resolution of microtubule 3D reconstructions and substantially reduces anisotropic blurring artifacts, compared with methods that utilize helical symmetry averaging. Moreover, we identified an unexpected, discrete behavior of the m-loop, which mediates lateral interactions between neighboring protofilaments and acts as a flexible hinge between them. The hinge angle adopts preferred values corresponding to distinct conformations of the m-loop that are incompatible with helical symmetry. These hinge angles fluctuate in a stochastic manner, and perfectly cylindrical microtubule conformations are thus energetically and entropically penalized. The hinge angle can diverge further from helical symmetry at the microtubule seam, generating a subpopulation of highly distorted microtubules. However, the seam-distorted subpopulation disappears in the presence of Taxol, a microtubule stabilizing agent. These observations provide clues into the structural origins of microtubule flexibility and dynamics and highlight the role of structural polymorphism in defining microtubule behavior.
		+
		+	Dynamic and asymmetric fluctuations in the microtubule wall captured by high-resolution cryoelectron microscopy.,Debs GE, Cha M, Liu X, Huehn AR, Sindelar CV Proc Natl Acad Sci U S A. 2020 Jul 7. pii: 2001546117. doi:, 10.1073/pnas.2001546117. PMID:32636254<ref>PMID:32636254</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6wvr" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>

OCA at 14:42, 8 July 2020

OCA — Wed, 08 Jul 2020 14:42:03 GMT

←Older revision		Revision as of 14:42, 8 July 2020
Line 1:		Line 1:

	==Tubulin dimers from a 13-protofilament, Taxol stabilized microtubule==		==Tubulin dimers from a 13-protofilament, Taxol stabilized microtubule==
-	<StructureSection load='6wvr' size='340' side='right'caption='[[6wvr]]' scene=''>	+	<StructureSection load='6wvr' size='340' side='right'caption='[[6wvr]], [[Resolution\|resolution]] 2.90Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WVR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WVR FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6wvr]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WVR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WVR FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wvr OCA], [http://pdbe.org/6wvr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wvr RCSB], [http://www.ebi.ac.uk/pdbsum/6wvr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wvr ProSAT]</span></td></tr>	+	</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wvr OCA], [http://pdbe.org/6wvr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wvr RCSB], [http://www.ebi.ac.uk/pdbsum/6wvr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wvr ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[[http://www.uniprot.org/uniprot/TBA1B_BOVIN TBA1B_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [[http://www.uniprot.org/uniprot/E1BJB1_BOVIN E1BJB1_BOVIN]] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.[RuleBase:RU000352][SAAS:SAAS00031082]
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Bos taurus]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: Cha M]]	+	[[Category: Cha, M]]
-	[[Category: Debs GE]]	+	[[Category: Debs, G E]]
-	[[Category: Huehn AR]]	+	[[Category: Huehn, A R]]
-	[[Category: Sindelar CV]]	+	[[Category: Sindelar, C V]]
		+	[[Category: Microtubule]]
		+	[[Category: Structural protein]]

OCA at 05:51, 20 May 2020

OCA — Wed, 20 May 2020 05:51:59 GMT

←Older revision		Revision as of 05:51, 20 May 2020
Line 1:		Line 1:
-	'''Unreleased structure'''

-	~~The entry~~ 6wvr ~~is ON HOLD~~	+	==Tubulin dimers from a 13-protofilament, Taxol stabilized microtubule==
-		+	<StructureSection load='6wvr' size='340' side='right'caption='[[6wvr]]' scene=''>
-	~~Authors~~: ~~Debs~~, G.E., ~~Cha~~, M., ~~Huehn, A~~.R., ~~Sindelar, C~~.V.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6WVR OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=6WVR FirstGlance]. <br>
-	~~Description: Tubulin dimers from a 13-protofilament, Taxol stabilized microtubule~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6wvr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6wvr OCA], [http://pdbe.org/6wvr PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6wvr RCSB], [http://www.ebi.ac.uk/pdbsum/6wvr PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6wvr ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
-	[[Category: ~~Debs, G.E~~]]	+	__TOC__
-	[[Category: ~~Sindelar, C.V~~]]	+	</StructureSection>
-	[[Category: ~~Cha, M~~]]	+	[[Category: Large Structures]]
-	[[Category: ~~Huehn, A.R~~]]	+	[[Category: Cha M]]
		+	[[Category: Debs GE]]
		+	[[Category: Huehn AR]]
		+	[[Category: Sindelar CV]]

OCA: Protected "6wvr" [edit=sysop:move=sysop]

OCA — Wed, 13 May 2020 04:11:59 GMT

Protected "6wvr" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:11, 13 May 2020

OCA: New page: '''Unreleased structure''' The entry 6wvr is ON HOLD Authors: Debs, G.E., Cha, M., Huehn, A.R., Sindelar, C.V. Description: Tubulin dimers from a 13-protofilament, Taxol stabilized mic...

OCA — Wed, 13 May 2020 04:11:58 GMT

New page: '''Unreleased structure''' The entry 6wvr is ON HOLD Authors: Debs, G.E., Cha, M., Huehn, A.R., Sindelar, C.V. Description: Tubulin dimers from a 13-protofilament, Taxol stabilized mic...

New page

'''Unreleased structure'''

The entry 6wvr is ON HOLD

Authors: Debs, G.E., Cha, M., Huehn, A.R., Sindelar, C.V.

Description: Tubulin dimers from a 13-protofilament, Taxol stabilized microtubule
[[Category: Unreleased Structures]]
[[Category: Debs, G.E]]
[[Category: Sindelar, C.V]]
[[Category: Cha, M]]
[[Category: Huehn, A.R]]