6x08 - Revision history

OCA at 14:40, 18 October 2023

OCA — Wed, 18 Oct 2023 14:40:04 GMT

←Older revision		Revision as of 14:40, 18 October 2023
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-	====	+	==Nup85-Seh1 from S. cerevisiae bound by VHH-SAN2==
-	<StructureSection load='6x08' size='340' side='right'caption='[[6x08]]' scene=''>	+	<StructureSection load='6x08' size='340' side='right'caption='[[6x08]], [[Resolution\|resolution]] 4.19Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6x08]] is a 3 chain structure with sequence from [https://en.wikipedia.org/wiki/Saccharomyces_cerevisiae_S288C Saccharomyces cerevisiae S288C] and [https://en.wikipedia.org/wiki/Vicugna_pacos Vicugna pacos]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X08 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X08 FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6x08 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x08 OCA], [~~http~~://pdbe.org/6x08 PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6x08 RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6x08 PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6x08 ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 4.19Å</td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x08 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x08 OCA], [https://pdbe.org/6x08 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x08 RCSB], [https://www.ebi.ac.uk/pdbsum/6x08 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x08 ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/SEH1_YEAST SEH1_YEAST] Functions as a component of the nuclear pore complex (NPC). NPC components, collectively referred to as nucleoporins (NUPs), can play the role of both NPC structural components and of docking or interaction partners for transiently associated nuclear transport factors. Involved in nuclear poly(A)+ RNA export and NPC biogenesis. It is also required for normal nuclear morphology. Component of the SEA complex which coats the vacuolar membrane and is involved in intracellular trafficking, autophagy, response to nitrogen starvation, and amino acid biogenesis.<ref>PMID:8565072</ref> <ref>PMID:11823431</ref> <ref>PMID:12206772</ref> <ref>PMID:21454883</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Nuclear pore complexes (NPCs) are the main conduits for molecular exchange across the nuclear envelope. The NPC is a modular assembly of ~500 individual proteins, called nucleoporins or nups. Most scaffolding nups are organized in two multimeric subcomplexes, the Nup84 or Y complex and the Nic96 or inner ring complex. Working in S. cerevisiae, and to study the assembly of these two essential subcomplexes, we here develop a set of twelve nanobodies that recognize seven constituent nucleoporins of the Y and Nic96 complexes. These nanobodies all bind specifically and with high affinity. We present structures of several nup-nanobody complexes, revealing their binding sites. Additionally, constitutive expression of the nanobody suite in S. cerevisiae detect accessible and obstructed surfaces of the Y complex and Nic96 within the NPC. Overall, this suite of nanobodies provides a unique and versatile toolkit for the study of the NPC.
		+
		+	A nanobody suite for yeast scaffold nucleoporins provides details of the nuclear pore complex structure.,Nordeen SA, Andersen KR, Knockenhauer KE, Ingram JR, Ploegh HL, Schwartz TU Nat Commun. 2020 Dec 2;11(1):6179. doi: 10.1038/s41467-020-19884-6. PMID:33268786<ref>PMID:33268786</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6x08" style="background-color:#fffaf0;"></div>
		+
		+	==See Also==
		+	*[[Antibody 3D structures\|Antibody 3D structures]]
		+	*[[Nucleoporin 3D structures\|Nucleoporin 3D structures]]
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Saccharomyces cerevisiae S288C]]
		+	[[Category: Vicugna pacos]]
		+	[[Category: Nordeen SA]]
		+	[[Category: Schwartz TU]]

OCA at 06:37, 9 December 2020

OCA — Wed, 09 Dec 2020 06:37:09 GMT

←Older revision		Revision as of 06:37, 9 December 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6x08 ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6x08' size='340' side='right'caption='[[6x08]]' scene=''>
-	~~Authors~~: ~~Nordeen, S~~.A.~~, Schwartz, T~~.U.	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~: ~~Nup85-Seh1 from S~~. ~~cerevisiae bound by VHH~~-~~SAN2~~	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6x08 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x08 OCA], [http://pdbe.org/6x08 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6x08 RCSB], [http://www.ebi.ac.uk/pdbsum/6x08 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6x08 ProSAT]</span></td></tr>
-	[[~~Category~~: ~~Unreleased Structures~~]]	+	</table>
-	[[Category: ~~Schwartz, T.U~~]]	+	__TOC__
-	[[Category: ~~Nordeen, S.A~~]]	+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 18:18, 24 June 2020

OCA — Wed, 24 Jun 2020 18:18:25 GMT

←Older revision		Revision as of 18:18, 24 June 2020
Line 1:		Line 1:
	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6x08 is ON HOLD	+	The entry 6x08 is ON HOLD until Paper Publication

	Authors: Nordeen, S.A., Schwartz, T.U.		Authors: Nordeen, S.A., Schwartz, T.U.

OCA: Protected "6x08" [edit=sysop:move=sysop]

OCA — Wed, 27 May 2020 04:47:48 GMT

Protected "6x08" [edit=sysop:move=sysop]

←Older revision

Revision as of 04:47, 27 May 2020

OCA: New page: '''Unreleased structure''' The entry 6x08 is ON HOLD Authors: Nordeen, S.A., Schwartz, T.U. Description: Nup85-Seh1 from S. cerevisiae bound by VHH-SAN2 [[Category: Unreleased Structur...

OCA — Wed, 27 May 2020 04:47:46 GMT

New page: '''Unreleased structure''' The entry 6x08 is ON HOLD Authors: Nordeen, S.A., Schwartz, T.U. Description: Nup85-Seh1 from S. cerevisiae bound by VHH-SAN2 [[Category: Unreleased Structur...

New page

'''Unreleased structure'''

The entry 6x08 is ON HOLD

Authors: Nordeen, S.A., Schwartz, T.U.

Description: Nup85-Seh1 from S. cerevisiae bound by VHH-SAN2
[[Category: Unreleased Structures]]
[[Category: Schwartz, T.U]]
[[Category: Nordeen, S.A]]