OCA at 14:43, 18 October 2023

OCA — Wed, 18 Oct 2023 14:43:50 GMT

←Older revision		Revision as of 14:43, 18 October 2023
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-	====	+	==Human Alpha-1,6-fucosyltransferase (FUT8) bound to GDP and A2-Asn==
-	<StructureSection load='6x5r' size='340' side='right'caption='[[6x5r]]' scene=''>	+	<StructureSection load='6x5r' size='340' side='right'caption='[[6x5r]], [[Resolution\|resolution]] 2.40Å' scene=''>
	== Structural highlights ==		== Structural highlights ==
-	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [~~http~~://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>	+	<table><tr><td colspan='2'>[[6x5r]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Gallus_gallus Gallus gallus] and [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6X5R OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6X5R FirstGlance]. <br>
-	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[~~http~~://proteopedia.org/fgij/fg.htm?mol=6x5r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x5r OCA], [~~http~~://pdbe.org/6x5r PDBe], [~~http~~://www.rcsb.org/pdb/explore.do?structureId=6x5r RCSB], [~~http~~://www.ebi.ac.uk/pdbsum/6x5r PDBsum], [~~http~~://prosat.h-its.org/prosat/prosatexe?pdbcode=6x5r ProSAT]</span></td></tr>	+	</td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models\|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution\|Resolution]] 2.4Å</td></tr>
		+	<tr id='ligand'><td class="sblockLbl"><b>[[Ligand\|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=BMA:BETA-D-MANNOSE'>BMA</scene>, <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MAN:ALPHA-D-MANNOSE'>MAN</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=NAG:N-ACETYL-D-GLUCOSAMINE'>NAG</scene></td></tr>
		+	<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6x5r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x5r OCA], [https://pdbe.org/6x5r PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6x5r RCSB], [https://www.ebi.ac.uk/pdbsum/6x5r PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6x5r ProSAT]</span></td></tr>
	</table>		</table>
		+	== Function ==
		+	[https://www.uniprot.org/uniprot/FUT8_HUMAN FUT8_HUMAN] Catalyzes the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans.<ref>PMID:9133635</ref>
		+	<div style="background-color:#fffaf0;">
		+	== Publication Abstract from PubMed ==
		+	Mammalian Asn-linked glycans are extensively processed as they transit the secretory pathway to generate diverse glycans on cell surface and secreted glycoproteins. Additional modification of the glycan core by alpha-1,6-fucose addition to the innermost GlcNAc residue (core fucosylation) is catalyzed by an alpha-1,6-fucosyltransferase (FUT8). The importance of core fucosylation can be seen in the complex pathological phenotypes of FUT8 null mice, which display defects in cellular signaling, development, and subsequent neonatal lethality. Elevated core fucosylation has also been identified in several human cancers. However, the structural basis for FUT8 substrate specificity remains unknown. Here, using various crystal structures of FUT8 in complex with a donor substrate analog, and with four distinct glycan acceptors, we identify the molecular basis for FUT8 specificity and activity. The ordering of three active site loops corresponds to an increased occupancy for bound GDP, suggesting an induced-fit folding of the donor binding subsite. Structures of the various acceptor complexes were compared with kinetic data on FUT8 active site mutants and with specificity data from a library of glycan acceptors to reveal how binding site complementarity and steric hindrance can tune substrate affinity. The FUT8 structure was also compared with other known fucosyltransferases to identify conserved and divergent structural features for donor and acceptor recognition and catalysis. These data provide insights into the evolution of modular templates for donor and acceptor recognition among GT-B fold glycosyltransferases in the synthesis of diverse glycan structures in biological systems.
		+
		+	Characterizing human alpha-1,6-fucosyltransferase (FUT8) substrate specificity and structural similarities with related fucosyltransferases.,Boruah BM, Kadirvelraj R, Liu L, Ramiah A, Li C, Zong G, Bosman GP, Yang JY, Wang LX, Boons GJ, Wood ZA, Moremen KW J Biol Chem. 2020 Oct 1. pii: RA120.014625. doi: 10.1074/jbc.RA120.014625. PMID:33004438<ref>PMID:33004438</ref>
		+
		+	From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
		+	</div>
		+	<div class="pdbe-citations 6x5r" style="background-color:#fffaf0;"></div>
		+	== References ==
		+	<references/>
	__TOC__		__TOC__
	</StructureSection>		</StructureSection>
		+	[[Category: Gallus gallus]]
		+	[[Category: Homo sapiens]]
	[[Category: Large Structures]]		[[Category: Large Structures]]
-	[[Category: ~~Z-disk~~]]	+	[[Category: Kadirvelraj R]]
		+	[[Category: Wood ZA]]

OCA at 06:19, 7 October 2020

OCA — Wed, 07 Oct 2020 06:19:54 GMT

←Older revision		Revision as of 06:19, 7 October 2020
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-	'''Unreleased structure'''

-	~~The entry~~ 6x5r ~~is ON HOLD until Paper Publication~~	+	====
-		+	<StructureSection load='6x5r' size='340' side='right'caption='[[6x5r]]' scene=''>
-	~~Authors~~:	+	== Structural highlights ==
-		+	<table><tr><td colspan='2'>Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id= OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol= FirstGlance]. <br>
-	~~Description~~:	+	</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=6x5r FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6x5r OCA], [http://pdbe.org/6x5r PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6x5r RCSB], [http://www.ebi.ac.uk/pdbsum/6x5r PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6x5r ProSAT]</span></td></tr>
-	[[Category: ~~Unreleased~~ Structures]]	+	</table>
		+	__TOC__
		+	</StructureSection>
		+	[[Category: Large Structures]]
		+	[[Category: Z-disk]]

OCA at 04:55, 10 June 2020

OCA — Wed, 10 Jun 2020 04:55:19 GMT

←Older revision		Revision as of 04:55, 10 June 2020
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	'''Unreleased structure'''		'''Unreleased structure'''

-	The entry 6x5r is ON HOLD	+	The entry 6x5r is ON HOLD until Paper Publication

	Authors:		Authors:

OCA: Protected "6x5r" [edit=sysop:move=sysop]

OCA — Wed, 03 Jun 2020 05:14:38 GMT

Protected "6x5r" [edit=sysop:move=sysop]

←Older revision

Revision as of 05:14, 3 June 2020

OCA: New page: '''Unreleased structure''' The entry 6x5r is ON HOLD Authors: Description: Category: Unreleased Structures

OCA — Wed, 03 Jun 2020 05:14:37 GMT

New page: '''Unreleased structure''' The entry 6x5r is ON HOLD Authors: Description: Category: Unreleased Structures

New page

'''Unreleased structure'''

The entry 6x5r is ON HOLD

Authors:

Description:
[[Category: Unreleased Structures]]

6x5r - Revision history

OCA at 14:43, 18 October 2023

OCA at 06:19, 7 October 2020

OCA at 04:55, 10 June 2020

OCA: Protected "6x5r" [edit=sysop:move=sysop]

OCA: New page: '''Unreleased structure''' The entry 6x5r is ON HOLD Authors: Description: Category: Unreleased Structures